FastQCFastQC Report
Thu 26 May 2016
SRR1512357_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512357_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1889602
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68380.36187514619480715No Hit
GGTATCAACGCAGAGTACTTTTTTT45020.23825122962401607No Hit
TATCAACGCAGAGTACTTTTTTTTT41300.21856454427969488No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19090.10102656538255146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12800.013.8889051
ATCGCGC803.0075878E-511.81961258
GTACCGT855.4184944E-511.1571816
TATCGCG855.4184944E-511.1571817
TAGGACC3100.010.707014
TGCCCCG803.8330827E-410.6687715
ATAGGAC2050.010.6398583
TTATACT2400.010.2736314
TCTATAC2053.6379788E-1210.1772573
GGACCGA750.002686225810.1158446
TCGCGCG750.002740019210.091699
ACTGCGC750.00275270510.0860698
CTACAGT10950.010.0462914
GTCCTAC8850.09.9323541
CGCGCGC1054.208621E-59.92992210
GTATTAT1905.2023097E-109.8765541
TCCTACA11350.09.775792
AGGACGT8850.09.5369315
TATGCTA1304.3342116E-69.4833535
TAGGACG8800.09.4833534
Produced by FastQC (version 0.11.5)