FastQCFastQC Report
Thu 26 May 2016
SRR1512357_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512357_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1889602
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT83670.4427916566557402No Hit
GGTATCAACGCAGAGTACTTTTTTT59820.31657460142400357No Hit
TATCAACGCAGAGTACTTTTTTTTT58520.30969484579292356No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26560.14055869966268028No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20380.10785339981646928No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAA250.005989949719.0233251
GGTATCA16350.014.1947751
GTGGTAT4800.011.6914191
TGGTATC4550.011.48969652
GGACCGT855.327891E-511.1756766
TTGTACG600.00585296611.0892984
TTAGAAC2100.010.8629853
TAGGACC4000.010.4556244
ACCGTGC750.002649584310.1326138
GTCTAAC1054.0505027E-59.9645991
GTATCAA32450.09.90737151
GTAATAC1252.5524805E-69.8853173
TGGCGAG8750.09.76737318
GGCGAGA5700.09.66265519
GTAGGAC9350.09.6576013
CTATACC1701.6500053E-89.5051124
CGCCCTA2002.6739144E-109.496057516
GTCCTAC8250.09.4540171
AGGACGT7900.09.3847955
TAGACAG4200.09.27885
Produced by FastQC (version 0.11.5)