##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512357_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1889602 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.270516225109837 33.0 31.0 34.0 28.0 34.0 2 31.452936120939754 33.0 31.0 34.0 28.0 34.0 3 31.558663676266217 34.0 31.0 34.0 28.0 34.0 4 35.090831296749265 37.0 35.0 37.0 32.0 37.0 5 34.83720645934964 37.0 35.0 37.0 32.0 37.0 6 34.85298226822368 37.0 35.0 37.0 32.0 37.0 7 34.73768550202635 37.0 35.0 37.0 32.0 37.0 8 34.69296920727222 37.0 35.0 37.0 32.0 37.0 9 36.23634818337406 39.0 37.0 39.0 32.0 39.0 10 36.12990989636971 39.0 37.0 39.0 32.0 39.0 11 36.186133376234785 39.0 37.0 39.0 32.0 39.0 12 36.0796993229262 39.0 37.0 39.0 32.0 39.0 13 36.04845358969772 39.0 37.0 39.0 32.0 39.0 14 37.25466473892386 40.0 38.0 41.0 32.0 41.0 15 37.21490345586002 40.0 38.0 41.0 31.0 41.0 16 37.14513003267355 40.0 38.0 41.0 31.0 41.0 17 37.12317038191111 40.0 38.0 41.0 31.0 41.0 18 37.05199190093999 40.0 37.0 41.0 31.0 41.0 19 37.07222790831085 40.0 38.0 41.0 31.0 41.0 20 37.03447128019551 40.0 38.0 41.0 31.0 41.0 21 36.98411199818798 40.0 38.0 41.0 31.0 41.0 22 36.905521903554295 40.0 37.0 41.0 31.0 41.0 23 36.81608560956222 40.0 37.0 41.0 31.0 41.0 24 36.732494991008686 40.0 37.0 41.0 31.0 41.0 25 36.64636574262728 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5709432524938833 1101 2 0.6191321712914437 1101 3 0.6118302943890761 1101 4 0.546648203852655 1101 5 0.5094109237153788 1101 6 0.6572666023509015 1101 7 0.6187186183714246 1101 8 0.6706026247373771 1101 9 0.6975195265882022 1101 10 0.6446791474626181 1101 11 0.6885941864355729 1101 12 0.5658012750007586 1101 13 0.6061099447823253 1101 14 0.8080275382147803 1101 15 0.839714409187863 1101 16 0.8964950339496269 1101 17 0.9272715760754267 1101 18 0.9479757138689422 1101 19 0.9506477451322581 1101 20 0.9969893583989631 1101 21 0.9813378904036583 1101 22 0.9014958567959326 1101 23 0.9634812182702959 1101 24 0.9746575705551734 1101 25 0.9681602910044447 1102 1 0.5824343211230882 1102 2 0.6064605116209698 1102 3 0.5558055250848604 1102 4 0.4811235629534707 1102 5 0.42064313410426735 1102 6 0.5062239482477935 1102 7 0.5303360438531755 1102 8 0.5836600771920644 1102 9 0.635130699348899 1102 10 0.6078392451659838 1102 11 0.6405675538141793 1102 12 0.5990011966447994 1102 13 0.6963736923921218 1102 14 0.8014452705031587 1102 15 0.9046240713907281 1102 16 0.9599546908744898 1102 17 0.8502771360920462 1102 18 0.8853035552832154 1102 19 0.9219921536839806 1102 20 0.8363281433880232 1102 21 0.8937323107455342 1102 22 0.8929334207030379 1102 23 0.8888406079341351 1102 24 1.003614349264197 1102 25 0.9608391035441315 1103 1 0.45098415274609494 1103 2 0.535116871003499 1103 3 0.5587520910562453 1103 4 0.503667155114961 1103 5 0.4205051551961887 1103 6 0.5016822053555288 1103 7 0.5642534711421874 1103 8 0.6531969229640708 1103 9 0.8093980602496984 1103 10 0.7536997899589863 1103 11 0.8016216121408064 1103 12 0.7777059994417783 1103 13 0.8099082169014764 1103 14 0.8835643349910853 1103 15 0.9950422279070636 1103 16 0.8722845514454249 1103 17 1.0622670256812725 1103 18 0.8991233116008956 1103 19 1.0228678045284738 1103 20 1.0990897472508294 1103 21 0.8418311297818022 1103 22 0.8826166698876676 1103 23 0.8750733791060412 1103 24 0.871605228079261 1103 25 0.9979378368776466 1104 1 0.3487011586002353 1104 2 0.33595616466010725 1104 3 0.3011146630309085 1104 4 0.09102990740192496 1104 5 0.09516310276137574 1104 6 0.11680391615864494 1104 7 0.24135356851083145 1104 8 0.18299317006404436 1104 9 0.17836150493589287 1104 10 0.23704758834579565 1104 11 0.14778636950055812 1104 12 0.13443657680176813 1104 13 0.15008372087435617 1104 14 0.0817619332312347 1104 15 0.2496011239149496 1104 16 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1107 16 0.9476891979734461 1107 17 0.8430976626762288 1107 18 1.000403262736448 1107 19 1.0402588811860554 1107 20 1.0848721193401403 1107 21 1.113303421081298 1107 22 1.0475537808844706 1107 23 1.2483644813606887 1107 24 1.1390715542348104 1107 25 1.1211762033052608 1108 1 0.16853024555281593 1108 2 0.0464380759847991 1108 3 0.040866680439556546 1108 4 0.04939467301984024 1108 5 0.12270911781260452 1108 6 -0.03542747381325029 1108 7 -0.018310045597431213 1108 8 0.01082932928065361 1108 9 0.033135283156944695 1108 10 -0.16316276617411063 1108 11 -0.08272863639299999 1108 12 0.021413183928430612 1108 13 0.1522974301861737 1108 14 0.09237395608008825 1108 15 0.13761227633130346 1108 16 0.29045710884271614 1108 17 0.27673644768673 1108 18 0.1984643259999217 1108 19 0.22100435063227764 1108 20 0.2871014951223074 1108 21 0.17059014634644853 1108 22 0.1414905543254079 1108 23 0.23862918974032254 1108 24 0.43649701098181737 1108 25 0.40748385273324317 1109 1 -0.19258174066943567 1109 2 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-1.714825415704233 2315 25 -1.5934345588079353 2316 1 -1.7296133399355647 2316 2 -1.703411772290817 2316 3 -1.4872169342254367 2316 4 -1.3466183306437145 2316 5 -1.3967293521825184 2316 6 -1.4679318398209986 2316 7 -1.6185866842357726 2316 8 -1.6162615615646558 2316 9 -1.983531932693083 2316 10 -1.7909467513880983 2316 11 -2.034322865773909 2316 12 -2.047207651888705 2316 13 -2.0128541144669327 2316 14 -2.208178122978353 2316 15 -2.125499601306643 2316 16 -2.4053632063485963 2316 17 -2.3940205117146434 2316 18 -2.6046358390546445 2316 19 -2.612174088029434 2316 20 -2.4961737745516928 2316 21 -2.6867365089171784 2316 22 -2.6113143997079433 2316 23 -2.82694517617756 2316 24 -2.6075449985500043 2316 25 -2.529500726197888 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25008.0 3 1.0 4 561.0 5 4097.0 6 4933.0 7 3746.0 8 2960.0 9 2727.0 10 2577.0 11 2291.0 12 2273.0 13 2160.0 14 2079.0 15 2136.0 16 2175.0 17 2304.0 18 2527.0 19 2811.0 20 3548.0 21 4637.0 22 5934.0 23 7177.0 24 8621.0 25 10424.0 26 11671.0 27 13072.0 28 14864.0 29 18400.0 30 23362.0 31 30344.0 32 39589.0 33 51500.0 34 69352.0 35 95044.0 36 146434.0 37 305411.0 38 962852.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.86877956590912 19.460900128571627 13.554183983558096 24.116136321961157 2 16.467764641129587 21.208230574637213 38.461900780084044 23.862104004149156 3 19.255642193435442 25.136457306882615 30.299025932445034 25.30887456723691 4 13.617194003563737 17.626213954463115 37.28900835049315 31.46758369148 5 13.59519368848495 37.716482921586504 35.21120046020581 13.477122929722738 6 31.927991144994365 37.2571927070072 17.642942330770033 13.171873817228407 7 27.86774782461866 31.934423348607805 22.130320149861664 18.067508676911874 8 25.842901808469588 35.632202159873856 20.359057988849703 18.16583804280685 9 26.689245881401952 14.827799961144553 19.772393892601215 38.71056026485228 10 15.42611308385687 28.61875329526181 34.01006629696211 21.945067323919208 11 34.662370297670186 22.31316803217724 24.04272653209016 18.981735138062408 12 23.279179170330202 24.860503315577205 31.09416959176079 20.766147922331804 13 29.172314902396902 21.54657472106405 25.724513598904952 23.5565967776341 14 21.62487291456842 21.555118162327457 27.61335544174393 29.206653481360185 15 23.696545008044712 29.393471081378607 23.675851045812514 23.234132864764163 16 22.943741042831405 27.775690270900768 26.415206176291896 22.86536250997593 17 22.2793128868901 27.812373815018837 27.211343007757783 22.696970290333283 18 22.18814663498431 26.92610566313327 28.677437962732654 22.20830973914976 19 23.52839380991341 26.67561740514669 27.84104800905164 21.954940775888257 20 23.77648838220959 26.37184973343593 28.00812022849256 21.843541655861923 21 23.65077265029636 26.02646062658764 27.575571549534295 22.747195173581712 22 23.668623082174744 26.576308143381087 27.615783986324498 22.13928478811967 23 23.149763730939576 26.569671721221034 27.58396958482374 22.69659496301565 24 23.281105376214345 26.24479982811186 27.97003176861147 22.504063027062326 25 23.18070154455806 26.461498241428615 27.777542572457058 22.580257641556265 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 431.0 1 431.0 2 620.5 3 810.0 4 810.0 5 810.0 6 1912.5 7 3015.0 8 3015.0 9 3015.0 10 4054.0 11 5093.0 12 5093.0 13 5093.0 14 7411.5 15 9730.0 16 9730.0 17 9730.0 18 16895.5 19 24061.0 20 24061.0 21 24061.0 22 35720.0 23 47379.0 24 47379.0 25 47379.0 26 66370.5 27 85362.0 28 85362.0 29 85362.0 30 105474.0 31 125586.0 32 125586.0 33 125586.0 34 143638.0 35 161690.0 36 161690.0 37 161690.0 38 176353.0 39 191016.0 40 191016.0 41 191016.0 42 205788.5 43 220561.0 44 220561.0 45 220561.0 46 231650.5 47 242740.0 48 242740.0 49 242740.0 50 240478.0 51 238216.0 52 238216.0 53 238216.0 54 217909.5 55 197603.0 56 197603.0 57 197603.0 58 171820.0 59 146037.0 60 146037.0 61 146037.0 62 121563.0 63 97089.0 64 97089.0 65 97089.0 66 75051.5 67 53014.0 68 53014.0 69 53014.0 70 38941.0 71 24868.0 72 24868.0 73 24868.0 74 17360.5 75 9853.0 76 9853.0 77 9853.0 78 6809.0 79 3765.0 80 3765.0 81 3765.0 82 2491.0 83 1217.0 84 1217.0 85 1217.0 86 788.5 87 360.0 88 360.0 89 360.0 90 226.0 91 92.0 92 92.0 93 92.0 94 52.5 95 13.0 96 13.0 97 13.0 98 7.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14373397149240952 2 0.003281114224053531 3 0.0 4 6.879755631079983E-4 5 0.09266501623093117 6 0.026513519778239014 7 0.020798030484726414 8 0.01719938907769996 9 0.029053737242022395 10 0.02783654970729286 11 0.029900476396616856 12 0.02587846541229317 13 0.019527921752834725 14 0.0066151496452692155 15 0.008573233940268902 16 0.002857744646756301 17 7.938179574323059E-4 18 6.350543659458447E-4 19 0.0 20 0.0 21 1.0584239432430745E-4 22 0.004868750138918142 23 0.001005502746080921 24 5.292119716215373E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1889602.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.94003157582301 #Duplication Level Percentage of deduplicated Percentage of total 1 82.09311760561158 58.23688355099838 2 12.09108380298666 17.154837335395918 3 3.07869265594182 6.552076626743015 4 1.1508107017591909 3.265541900823682 5 0.5287852891985992 1.8756022556289669 6 0.293288665559583 1.248354431737669 7 0.1790337339382061 0.8890461117099688 8 0.11397885442195436 0.6468530825335663 9 0.08505187904609043 0.5430224686601461 >10 0.34976392176135657 4.283428128015551 >50 0.022004695219226413 1.078261286135493 >100 0.012152512932864297 1.7383566774308195 >500 0.00111784081140587 0.5142318778185064 >1k 8.94272649124692E-4 0.9055492788273019 >5k 2.23568162281173E-4 1.0679549875410312 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8367 0.4427916566557402 No Hit GGTATCAACGCAGAGTACTTTTTTT 5982 0.31657460142400357 No Hit TATCAACGCAGAGTACTTTTTTTTT 5852 0.30969484579292356 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2656 0.14055869966268028 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2038 0.10785339981646928 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.292119716215373E-5 7 0.0 0.0 0.0 0.0 5.292119716215373E-5 8 0.0 0.0 0.0 0.0 5.292119716215373E-5 9 0.0 0.0 0.0 0.0 5.292119716215373E-5 10 0.0 0.0 0.0 0.0 5.292119716215373E-5 11 0.0 0.0 0.0 0.0 5.292119716215373E-5 12 0.0 0.0 0.0 0.0 1.5876359148646118E-4 13 0.0 0.0 0.0 0.0 1.5876359148646118E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGTAA 25 0.0059899497 19.023325 1 GGTATCA 1635 0.0 14.194775 1 GTGGTAT 480 0.0 11.691419 1 TGGTATC 455 0.0 11.4896965 2 GGACCGT 85 5.327891E-5 11.175676 6 TTGTACG 60 0.005852966 11.089298 4 TTAGAAC 210 0.0 10.862985 3 TAGGACC 400 0.0 10.455624 4 ACCGTGC 75 0.0026495843 10.132613 8 GTCTAAC 105 4.0505027E-5 9.964599 1 GTATCAA 3245 0.0 9.9073715 1 GTAATAC 125 2.5524805E-6 9.885317 3 TGGCGAG 875 0.0 9.767373 18 GGCGAGA 570 0.0 9.662655 19 GTAGGAC 935 0.0 9.657601 3 CTATACC 170 1.6500053E-8 9.505112 4 CGCCCTA 200 2.6739144E-10 9.4960575 16 GTCCTAC 825 0.0 9.454017 1 AGGACGT 790 0.0 9.384795 5 TAGACAG 420 0.0 9.2788 5 >>END_MODULE