FastQCFastQC Report
Thu 26 May 2016
SRR1512356_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512356_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1721556
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT70750.41096542894916No Hit
GGTATCAACGCAGAGTACTTTTTTT45950.2669097026178643No Hit
TATCAACGCAGAGTACTTTTTTTTT42010.24402342996684398No Hit
GTCCTACAGTGGACATTTCTAAATT32070.18628496546147788No Hit
GTCCTAAAGTGTGTATTTCTCATTT31030.18024391887339128No Hit
CTGTAGGACGTGGAATATGGCAAGA29220.16973017433066365No Hit
CTTTAGGACGTGAAATATGGCGAGG27000.15683486334455574No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20490.11902023518259063No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18940.11001675228688465No Hit
GTCCTACAGTGTGCATTTCTCATTT17850.10368527076667852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11850.014.8399231
CGTGCGC802.0514453E-613.03283110
GTCGTAG550.002287757812.5737651
TAGGACC5150.012.1531344
GTCTTAG2100.011.7611751
CCAATCG658.110858E-411.67733615
GCGCCAC855.4077384E-511.15922613
GGCGAGG9550.010.91628219
CCTATAG2400.010.6685183
GCTCTAA1302.2416134E-710.6393391
TTAGGAC12750.010.6359783
ATTTAGA5800.010.5607241
ATTAGAC1451.4835678E-810.464143
AATCCCG1451.4977559E-810.45772919
TAGACAG2450.010.4507935
GGGTAAG1251.55628E-610.2745631
GTCCCGC2400.010.26919112
AGGACCT11200.010.2451645
GACGTGA11550.010.1816157
GTATCAA25550.010.0920541
Produced by FastQC (version 0.11.5)