FastQCFastQC Report
Thu 26 May 2016
SRR1512356_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512356_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1721556
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT88830.5159867004035884No Hit
GGTATCAACGCAGAGTACTTTTTTT64140.37256993092295576No Hit
TATCAACGCAGAGTACTTTTTTTTT59610.34625652607292473No Hit
CTGTAGGACGTGGAATATGGCAAGA33340.19366201273731437No Hit
GTCCTACAGTGGACATTTCTAAATT33110.19232601204956448No Hit
GTCCTAAAGTGTGTATTTCTCATTT32480.18866653190485816No Hit
CTTTAGGACGTGAAATATGGCGAGG30020.1743771332445764No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29010.16851034761576156No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22010.12784945711902487No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19390.11263066667596057No Hit
CTGAAGGACCTGGAATATGGCGAGA18120.10525361940012408No Hit
GTCCTACAGTGTGCATTTCTCATTT17690.10275587898389597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14800.014.53303051
ACCGTGC551.964111E-413.8150568
TAGGACC6300.012.6755854
CTTATAC1603.6379788E-1211.8833613
TGGTATC3650.011.4604512
TTAGACT1751.8189894E-1211.4080274
CCCCGTA1751.8189894E-1211.39477215
GGCGAGG8350.011.37202719
AATGTCC5650.011.0948338
CGTGCGC700.00149436210.85468610
GTCCTAT1855.456968E-1210.8033141
AGGACCT13450.010.7436185
GTGGTAT4000.010.7068571
GTAGTAA803.7085795E-410.7068561
TCCAACG1252.2541644E-710.6351218
AAATGTC6200.010.4169967
TGTCCAC6850.010.26045110
GAAATGT6500.010.0823336
CCAACGA856.621577E-410.05421119
TTAGGAC13250.010.0448043
Produced by FastQC (version 0.11.5)