##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512356_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1721556 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.534525742990642 33.0 31.0 34.0 29.0 34.0 2 31.717102435238818 34.0 31.0 34.0 29.0 34.0 3 31.82917953293416 34.0 31.0 34.0 28.0 34.0 4 35.32215275018646 37.0 35.0 37.0 33.0 37.0 5 35.09844408198165 37.0 35.0 37.0 32.0 37.0 6 35.117306669083085 37.0 35.0 37.0 32.0 37.0 7 35.00739098815258 37.0 35.0 37.0 32.0 37.0 8 34.966723708087336 37.0 35.0 37.0 32.0 37.0 9 36.545401369458794 39.0 37.0 39.0 32.0 39.0 10 36.45877101877604 39.0 37.0 39.0 32.0 39.0 11 36.50401555337149 39.0 37.0 39.0 32.0 39.0 12 36.412969429980784 39.0 37.0 39.0 32.0 39.0 13 36.388588579169074 39.0 37.0 39.0 32.0 39.0 14 37.63817035286682 40.0 38.0 41.0 32.0 41.0 15 37.615499002065576 40.0 38.0 41.0 32.0 41.0 16 37.562498693042805 40.0 38.0 41.0 32.0 41.0 17 37.549770091707735 40.0 38.0 41.0 32.0 41.0 18 37.49420001440557 40.0 38.0 41.0 32.0 41.0 19 37.51578746204015 40.0 38.0 41.0 32.0 41.0 20 37.48788479724156 40.0 38.0 41.0 32.0 41.0 21 37.43867640669255 40.0 38.0 41.0 32.0 41.0 22 37.371825255757 40.0 38.0 41.0 32.0 41.0 23 37.282806368192496 40.0 38.0 41.0 32.0 41.0 24 37.21839719416621 40.0 38.0 41.0 32.0 41.0 25 37.144471048284224 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.45942282079849406 1101 2 0.4752921017269429 1101 3 0.4154845766508686 1101 4 0.3789302212724408 1101 5 0.39400841303988443 1101 6 0.4909963248210474 1101 7 0.5303581785307188 1101 8 0.5604971844871613 1101 9 0.5965299267949931 1101 10 0.6095310311121409 1101 11 0.6917531154953025 1101 12 0.6316059222123087 1101 13 0.7637278874141984 1101 14 0.8205814570918335 1101 15 0.6753988407833731 1101 16 0.839604368855909 1101 17 0.8134053912785433 1101 18 0.7409551832676016 1101 19 0.6918278572830161 1101 20 0.7505115140920822 1101 21 0.7510335861363018 1101 22 0.7990281225626887 1101 23 0.8857634571762887 1101 24 0.7876163006548822 1101 25 0.7795716116925675 1102 1 0.5774288487709924 1102 2 0.5380197834732172 1102 3 0.5549532829589552 1102 4 0.44473284629481213 1102 5 0.44062602381476523 1102 6 0.528215910410033 1102 7 0.5242740868629596 1102 8 0.5235388432923074 1102 9 0.6964617129809696 1102 10 0.645152188037784 1102 11 0.6741394378999104 1102 12 0.7502921531813769 1102 13 0.7682822480185436 1102 14 0.8785273988536844 1102 15 0.721758109879616 1102 16 0.8459235804783845 1102 17 0.9337693918010359 1102 18 0.9502071051071539 1102 19 0.9256493306046423 1102 20 0.8792953246802213 1102 21 0.8731972702627999 1102 22 0.8707842170557498 1102 23 0.7825099968975309 1102 24 0.7573883891102966 1102 25 0.8011978096674355 1103 1 0.31237821313212066 1103 2 0.37345395714267937 1103 3 0.2985430265486997 1103 4 0.2576503209769925 1103 5 0.32705136318127614 1103 6 0.33998061629238663 1103 7 0.3542891698192179 1103 8 0.40575172844636853 1103 9 0.4350417603845429 1103 10 0.3653741229655978 1103 11 0.3440704872427247 1103 12 0.4408545319790491 1103 13 0.5063990331941497 1103 14 0.6653729062874234 1103 15 0.6242467976624226 1103 16 0.6178692818358513 1103 17 0.5820240782910133 1103 18 0.6056154222801737 1103 19 0.5666426510097224 1103 20 0.6689634996425085 1103 21 0.5523289229238628 1103 22 0.626569608135263 1103 23 0.5891572711918585 1103 24 0.5655762796511254 1103 25 0.6459295485168255 1104 1 0.48996146638077676 1104 2 0.37005906306337977 1104 3 0.3839230457690235 1104 4 0.35511616311964644 1104 5 0.27249489303545715 1104 6 0.303478715151698 1104 7 0.3553462876063733 1104 8 0.30768002828341423 1104 9 0.4668827331000429 1104 10 0.37610616658737683 1104 11 0.2616675289842547 1104 12 0.35079060351208113 1104 13 0.27745991147936877 1104 14 0.3908001406500219 1104 15 0.43357074367838067 1104 16 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1107 16 0.9521723536250164 1107 17 1.0089911601524264 1107 18 1.0478864438059219 1107 19 0.9509775041432462 1107 20 0.9819101192075976 1107 21 0.9077794234800365 1107 22 1.0682875120333648 1107 23 0.9941622217474233 1107 24 0.9952038267425252 1107 25 1.1322061195542403 1108 1 0.39277933769515627 1108 2 0.3778834923194907 1108 3 0.3642695159389149 1108 4 0.3313277333414888 1108 5 0.3218146175708938 1108 6 0.10854759679501313 1108 7 0.23024336611592133 1108 8 0.19165505708451747 1108 9 0.2575948948473794 1108 10 0.22632435591894762 1108 11 0.2131412970763762 1108 12 0.22558225884481686 1108 13 0.33294830125873176 1108 14 0.4265791883292067 1108 15 0.42250948463813387 1108 16 0.5132028014558259 1108 17 0.5395065283785527 1108 18 0.48024973970120755 1108 19 0.4318442112553953 1108 20 0.40287517696363295 1108 21 0.2729568419690125 1108 22 0.3733889538129347 1108 23 0.3606984700034275 1108 24 0.5350454197481866 1108 25 0.4849915762659336 1109 1 -0.15830979451240879 1109 2 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-1.5624879276052184 2315 17 -1.5492035162182063 2315 18 -1.6219085079824822 2315 19 -1.4394226118902438 2315 20 -1.378380600755392 2315 21 -1.3406182413999375 2315 22 -1.330944520973631 2315 23 -1.3866575828828118 2315 24 -1.5905134861332542 2315 25 -1.5956656957929098 2316 1 -1.5239753258020627 2316 2 -1.4205706204302828 2316 3 -1.2806841978398182 2316 4 -1.200916381138093 2316 5 -1.2823991862692665 2316 6 -1.3130454973788872 2316 7 -1.242990754557475 2316 8 -1.295584677083589 2316 9 -1.5529825808656028 2316 10 -1.5691136473286207 2316 11 -1.7465888534166183 2316 12 -1.6108788958834097 2316 13 -1.5518261824504194 2316 14 -1.8538011454672727 2316 15 -1.9783846569045167 2316 16 -2.0064483360301537 2316 17 -2.0496443607967763 2316 18 -2.191601876550024 2316 19 -2.2323447057195693 2316 20 -2.0761469812744195 2316 21 -2.340681829764307 2316 22 -2.3118163070248485 2316 23 -2.32983481199377 2316 24 -2.2563497513071624 2316 25 -2.139295548966338 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18126.0 3 1.0 4 366.0 5 3054.0 6 3821.0 7 2954.0 8 2403.0 9 2155.0 10 2010.0 11 1872.0 12 1708.0 13 1596.0 14 1695.0 15 1743.0 16 1610.0 17 1804.0 18 1970.0 19 2317.0 20 2919.0 21 3689.0 22 4793.0 23 5897.0 24 7120.0 25 8340.0 26 9494.0 27 10891.0 28 12723.0 29 15254.0 30 19575.0 31 25329.0 32 33116.0 33 43739.0 34 58655.0 35 80499.0 36 121026.0 37 243441.0 38 963851.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.261933850410614 19.79792796755119 13.57397672454116 24.366161457497036 2 15.33130252027618 21.131202373774162 40.50798209445604 23.029513011493613 3 18.772610359465506 25.69576592338559 30.544170506216467 24.987453210932436 4 12.858668900326975 17.85919459274521 38.199773574691015 31.082362932236794 5 12.854669107300271 38.31153199377592 35.77189301837686 13.061905880546956 6 31.014030282447536 37.885481240372954 18.144077235987293 12.956411241192217 7 27.144419228628436 32.146969860708765 22.761573420438292 17.947037490224513 8 24.78728812328429 37.024444799488734 20.82940928699037 17.358857790236605 9 26.428272186206613 14.87333332171227 20.557718041641753 38.140676450439365 10 15.304267289690305 28.82121518660786 34.62475937919979 21.249758144502053 11 33.878752980100515 22.283743337445287 25.237202345846804 18.6003013366074 12 23.35655729846659 24.909373080769083 32.03994963589448 19.69411998486985 13 29.18852905240175 22.077315427819123 26.05870497210349 22.67545054767564 14 20.83562067981971 22.58992103644244 28.090648049554233 28.483810234183615 15 22.83229455733271 31.354459727073685 23.782616298084662 22.030629417508948 16 21.783674137997135 28.07180191389672 28.276912066936426 21.867611881169715 17 21.24880266877796 28.771673219919176 28.174706672002987 21.80481743929988 18 21.05787472423043 27.00839826528016 30.845007917302237 21.08871909318717 19 23.21498690719326 26.622950400684033 28.552542002699884 21.609520689422823 20 23.497057313267764 26.890905669057524 28.965366215214612 20.6466708024601 21 22.817613833067295 26.46692875514941 28.12060717165169 22.594850240131603 22 23.06466163491762 27.846624017045908 28.28258888193746 20.80612546609901 23 21.943969901349895 27.610992257509125 28.796907883004685 21.648129958136295 24 22.902885529137595 26.53878235735579 29.2721816775057 21.28615043600092 25 22.36523238279789 27.2652182095732 29.028622943430243 21.340926464198667 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 427.0 1 427.0 2 635.0 3 843.0 4 843.0 5 843.0 6 2040.0 7 3237.0 8 3237.0 9 3237.0 10 4264.0 11 5291.0 12 5291.0 13 5291.0 14 7322.5 15 9354.0 16 9354.0 17 9354.0 18 16377.0 19 23400.0 20 23400.0 21 23400.0 22 35894.0 23 48388.0 24 48388.0 25 48388.0 26 69770.5 27 91153.0 28 91153.0 29 91153.0 30 114722.5 31 138292.0 32 138292.0 33 138292.0 34 154166.5 35 170041.0 36 170041.0 37 170041.0 38 179775.5 39 189510.0 40 189510.0 41 189510.0 42 198553.5 43 207597.0 44 207597.0 45 207597.0 46 213700.0 47 219803.0 48 219803.0 49 219803.0 50 211067.0 51 202331.0 52 202331.0 53 202331.0 54 179444.5 55 156558.0 56 156558.0 57 156558.0 58 134740.5 59 112923.0 60 112923.0 61 112923.0 62 93085.0 63 73247.0 64 73247.0 65 73247.0 66 56525.0 67 39803.0 68 39803.0 69 39803.0 70 29089.0 71 18375.0 72 18375.0 73 18375.0 74 12815.5 75 7256.0 76 7256.0 77 7256.0 78 4900.0 79 2544.0 80 2544.0 81 2544.0 82 1694.0 83 844.0 84 844.0 85 844.0 86 552.0 87 260.0 88 260.0 89 260.0 90 164.5 91 69.0 92 69.0 93 69.0 94 38.5 95 8.0 96 8.0 97 8.0 98 5.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15474373183329498 2 0.004530784941064943 3 0.0 4 9.874787692064622E-4 5 0.10362718378025462 6 0.028636884306987402 7 0.024048012379498548 8 0.019226792506314053 9 0.031541233628182876 10 0.028288362388443945 11 0.03235445143811761 12 0.02840453636129176 13 0.021666445936118255 14 0.00749322124868433 15 0.008074091112923424 16 0.0024396534298042004 17 0.0011036527420542812 18 5.808698642390954E-4 19 0.0 20 0.0 21 0.0 22 0.005053567818880129 23 9.874787692064622E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1721556.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.39709059149514 #Duplication Level Percentage of deduplicated Percentage of total 1 82.9327914481939 56.72361649687693 2 11.692524666557198 15.994693377236086 3 2.8399353370180287 5.827299435600313 4 0.9870089657363696 2.7003416657635366 5 0.4707082986859589 1.6097539073696048 6 0.2493361755864951 1.023232139359587 7 0.16780498870798113 0.8034161110055225 8 0.11263906523694936 0.6163347479322367 9 0.08067845186609823 0.49663542429603913 >10 0.4098685554254103 5.03731461845343 >50 0.03006085110315383 1.4266064310663173 >100 0.021892051710780756 3.081694069276636 >500 0.002884623247107789 1.3448885397556523 >1k 0.0016119953439719997 2.0805848163789764 >5k 2.545255806271578E-4 1.2335882196291705 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8883 0.5159867004035884 No Hit GGTATCAACGCAGAGTACTTTTTTT 6414 0.37256993092295576 No Hit TATCAACGCAGAGTACTTTTTTTTT 5961 0.34625652607292473 No Hit CTGTAGGACGTGGAATATGGCAAGA 3334 0.19366201273731437 No Hit GTCCTACAGTGGACATTTCTAAATT 3311 0.19232601204956448 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3248 0.18866653190485816 No Hit CTTTAGGACGTGAAATATGGCGAGG 3002 0.1743771332445764 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2901 0.16851034761576156 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2201 0.12784945711902487 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1939 0.11263066667596057 No Hit CTGAAGGACCTGGAATATGGCGAGA 1812 0.10525361940012408 No Hit GTCCTACAGTGTGCATTTCTCATTT 1769 0.10275587898389597 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 5.8086986423909534E-5 0.0 11 0.0 0.0 0.0 5.8086986423909534E-5 0.0 12 0.0 0.0 0.0 5.8086986423909534E-5 0.0 13 0.0 0.0 0.0 5.8086986423909534E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1480 0.0 14.5330305 1 ACCGTGC 55 1.964111E-4 13.815056 8 TAGGACC 630 0.0 12.675585 4 CTTATAC 160 3.6379788E-12 11.883361 3 TGGTATC 365 0.0 11.460451 2 TTAGACT 175 1.8189894E-12 11.408027 4 CCCCGTA 175 1.8189894E-12 11.394772 15 GGCGAGG 835 0.0 11.372027 19 AATGTCC 565 0.0 11.094833 8 CGTGCGC 70 0.001494362 10.854686 10 GTCCTAT 185 5.456968E-12 10.803314 1 AGGACCT 1345 0.0 10.743618 5 GTGGTAT 400 0.0 10.706857 1 GTAGTAA 80 3.7085795E-4 10.706856 1 TCCAACG 125 2.2541644E-7 10.63512 18 AAATGTC 620 0.0 10.416996 7 TGTCCAC 685 0.0 10.260451 10 GAAATGT 650 0.0 10.082333 6 CCAACGA 85 6.621577E-4 10.054211 19 TTAGGAC 1325 0.0 10.044804 3 >>END_MODULE