FastQCFastQC Report
Thu 26 May 2016
SRR1512355_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512355_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1841364
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69610.37803497841817263No Hit
GGTATCAACGCAGAGTACTTTTTTT44370.24096267766720758No Hit
TATCAACGCAGAGTACTTTTTTTTT41770.22684271007796394No Hit
GTCCTAAAGTGTGTATTTCTCATTT22030.11963957153501427No Hit
GTCCTACAGTGGACATTTCTAAATT21990.11942234126441052No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20460.11111328341381714No Hit
CTGTAGGACGTGGAATATGGCAAGA19030.10334730123973315No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18630.10117499853369567No Hit
GTACTGGTTCACTATCGGTCAGTCA18520.10057761528953536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12800.014.74642851
GGACCGT400.005341775214.2248646
TAGGACC5550.011.960524
GCGCAGA958.778035E-611.4428051
CTTATAC2050.011.1023333
ACGGCAG600.00595406311.0637835
GTATTAA2600.011.0227031
GTCCTAC13550.010.79408551
GTCCTAA7350.010.6219241
TCCTACA15100.010.4252852
GTATAGA2400.010.294191
GTCTAAT1552.2037057E-810.2012111
GTATATA3500.010.1647781
GATCAAC1853.1650416E-1010.1495161
TGTAGGA16950.010.1266482
GTATAAG2155.456968E-1210.11224651
TAAAGGT2950.09.9651694
GGACCTG10500.09.9348256
CTAAGAC2201.8189894E-129.9142993
GTATTAG1708.72933E-99.8824231
Produced by FastQC (version 0.11.5)