FastQCFastQC Report
Thu 26 May 2016
SRR1512355_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512355_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1841364
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT85550.4646012412537663No Hit
GGTATCAACGCAGAGTACTTTTTTT57640.31302881994000104No Hit
TATCAACGCAGAGTACTTTTTTTTT55580.30184146100390796No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27470.149182888337124No Hit
CTGTAGGACGTGGAATATGGCAAGA23090.12539617370601358No Hit
GTCCTACAGTGGACATTTCTAAATT22180.12045418504977833No Hit
GTCCTAAAGTGTGTATTTCTCATTT22100.12001972450857083No Hit
CTTTAGGACGTGAAATATGGCGAGG20320.11035297746670404No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20270.11008143962844934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13700.013.9604331
GGACCGT802.0022526E-613.0611746
TAGGACC5850.012.350024
TGGACCG1209.915311E-911.8831575
TGCGCCA1052.7291026E-711.75911312
CCCCGGT1157.125709E-811.55933319
GGTCTAT1406.6211214E-1011.5543171
GTCCTAC13350.010.7626471
GCGCCAC1158.8542583E-710.73570713
GTATCAA29300.010.6194871
CGCCCTA3400.010.61224516
TTAGGAC8400.010.4116253
TGTAGGA14900.010.3994722
GTAGGAC15200.010.3193033
GGCGAGG5800.010.31371619
TTGCACT1303.8069265E-710.2375174
GGACTGT2800.010.1775386
TGGCGAG12000.010.04905118
TAGGACA4850.09.9962814
GGACGTG13600.09.9879576
Produced by FastQC (version 0.11.5)