FastQCFastQC Report
Thu 26 May 2016
SRR1512354_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512354_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164858
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58133.526064855815308No Hit
GGTATCAACGCAGAGTACTTTTTTT42452.5749432845236506No Hit
TATCAACGCAGAGTACTTTTTTTTT36692.2255516869063072No Hit
GTACTGGTTCACTATCGGTCAGTCA25851.568016110834779No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18831.1421951012386418No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16521.002074512610853No Hit
GGATACCACGTGTCCCGCCCTACTC15210.9226121874582974No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12940.7849179293695181No Hit
ATCATTAACTGAATCCATAGGTTAA12650.7673270329617005No Hit
ATACAGGGTGACAGCCCCGTACACA12280.7448834754758641No Hit
GTATTTAGCCTTGGAGGATGGTCCC10770.6532894976282619No Hit
GTACATGGGGGTTAAGCGACTAAGC10010.6071892173870846No Hit
GTTCACTATCGGTCAGTCAGGAGTA8740.5301532227735385No Hit
GGTTAATGAGGCGAACCGGGGGAAC8510.5162018221742347No Hit
GTGGATGCCCTGGCAGTCAGAGGCG8370.5077096652877021No Hit
GCAGAGTACTTTTTTTTTTTTTTTT8030.48708585570612284No Hit
GGTATCAACGCAGAGTACATGGGGG7850.47616736828058087No Hit
CCCATATTCAGACAGGATACCACGT7770.47131470720256224No Hit
GCCTTGGAGGATGGTCCCCCCATAT7760.47070812456780986No Hit
TATTCAGACAGGATACCACGTGTCC7690.46646204612454356No Hit
GTCAGGAGTATTTAGCCTTGGAGGA7580.4597896371422679No Hit
CTATCGGTCAGTCAGGAGTATTTAG7390.4482645670819736No Hit
GTGGTATCAACGCAGAGTACATGGG7360.44644481917771656No Hit
GTATCAACGCAGAGTACATGGGGGT7110.43128025330890823No Hit
GTGGTATCAACGCAGAGTACTTTTT6980.4233946790571279No Hit
GCCCAGAGCCTGAATCAGTGTGTGT6830.414295939535843No Hit
TCTAAGTACCCCGAGGAAAAGAAAT6710.40701694791881493No Hit
AGCGTACACGGTGGATGCCCTGGCA6610.40095112157129165No Hit
ACCCTGTATCGCGCGCCTTTCCAGA6500.394278712589016No Hit
CGGTACTGGTTCACTATCGGTCAGT6420.38942605151099735No Hit
GGTTCACTATCGGTCAGTCAGGAGT6050.36698249402516103No Hit
ACTTAGATGTTTCAGTTCCCCCGGT5560.3372599449222968No Hit
TCACGGTACTGGTTCACTATCGGTC5530.3354401970180398No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG5380.3263414574967548No Hit
GTTAATGATAGTGTGTCGAAACACA5240.31784930061022215No Hit
ATCAACGCAGAGTACTTTTTTTTTT4940.29965182156765213No Hit
GGAGTATTTAGCCTTGGAGGATGGT4910.2978320736633952No Hit
GCCTGAATCAGTGTGTGTGTTAGTG4740.2875201688726055No Hit
CATCTAAGTACCCCGAGGAAAAGAA4740.2875201688726055No Hit
GTTATAACCGGCGATTTCCGAATGG4690.28448725569884387No Hit
ACGCAGAGTACATGGGGGTTAAGCG4590.2784214293513205No Hit
GAATCAGTGTGTGTGTTAGTGGAAG4500.2729621856385495No Hit
GAGTACATGGGGGTTAAGCGACTAA4410.26750294192577856No Hit
TATCAACGCAGAGTACATGGGGGTT4280.25961736767399823No Hit
GTGCTAATCTGCGATAAGCGTCGGT4250.25779761976974125No Hit
GTCCCGCCCTACTCATCGAGCTCAC4150.2517317934222179No Hit
GTACTTAGATGTTTCAGTTCCCCCG3960.24020672336192359No Hit
GGTGATATGAACCGTTATAACCGGC3960.24020672336192359No Hit
GATATGAACCGTTATAACCGGCGAT3860.23414089701440025No Hit
GCCCTACTCATCGAGCTCACAGCAT3610.21897633114559195No Hit
CACTATCATTAACTGAATCCATAGG3600.21836974851083962No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG3570.21655000060658267No Hit
GGGTGACAGCCCCGTACACAAAAAT3470.21048417425905933No Hit
ATCCATAGGTTAATGAGGCGAACCG3460.209877591624307No Hit
CACACACACTGATTCAGGCTCTGGG3440.2086644263548023No Hit
CTATCATTAACTGAATCCATAGGTT3360.20381176527678366No Hit
GGTATCAACGCAGAGTACATGGGGA3290.19956568683351736No Hit
CCCCAGTAGCGGCGAGCGAACGGGG3270.19835252156401267No Hit
AGCTCACAGCATGTGCATTTTTGTG3240.19653277365975566No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA3240.19653277365975566No Hit
ATTTAGCCTTGGAGGATGGTCCCCC3200.19410644312074635No Hit
GCGTACACGGTGGATGCCCTGGCAG3190.193499860485994No Hit
TTTGTGTACGGGGCTGTCACCCTGT3110.18864719940797534No Hit
TCATTAACCTATGGATTCAGTTAAT3090.18743403413847068No Hit
TCGGTAAGGTGATATGAACCGTTAT2950.17894187725193803No Hit
GGGTACTTAGATGTTTCAGTTCCCC2900.17590896407817638No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA2770.16802338982639606No Hit
GTGGTATCCTGTCTGAATATGGGGG2770.16802338982639606No Hit
AGTCAGGAGTATTTAGCCTTGGAGG2680.16256414611362507No Hit
NTATCAACGCAGAGTACTTTTTTTT2620.15892465030511108No Hit
GATTCAGGCTCTGGGCTGCTCCCCG2570.1558917371313494No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA2540.15407198922709242No Hit
CCGTTATAACCGGCGATTTCCGAAT2530.15346540659234006No Hit
GGGAAACCCAGTGTGTTTCGACACA2500.15164565868808308No Hit
TTCCAGACGCTTCCACTAACACACA2490.15103907605333075No Hit
TAAGTACCCCGAGGAAAAGAAATCA2480.15043249341857842No Hit
CCTATGGATTCAGTTAATGATAGTG2460.14921932814907377No Hit
CAGTAGCGGCGAGCGAACGGGGAGC2430.14739958024481675No Hit
GGGCTGTCACCCTGTATCGCGCGCC2420.1467929976100644No Hit
GTCCCCCCATATTCAGACAGGATAC2410.1461864149753121No Hit
GTGTTTCGACACACTATCATTAACT2400.14557983234055977No Hit
CCACGTGTCCCGCCCTACTCATCGA2380.14436666707105508No Hit
GAATATGGGGGGACCATCCTCCAAG2340.14194033653204574No Hit
TCCCCCAGTAGCGGCGAGCGAACGG2330.1413337538972934No Hit
GTGCATTTTTGTGTACGGGGCTGTC2300.13951400599303643No Hit
CCCTGGCAGTCAGAGGCGATGAAGG2290.1389074233582841No Hit
AAGTACCCCGAGGAAAAGAAATCAA2250.13648109281927476No Hit
GTACATGGGCATCTAAGTACCCCGA2210.13405476228026544No Hit
CATTAACTGAATCCATAGGTTAATG2200.1334481796455131No Hit
GGAGGATGGTCCCCCCATATTCAGA2200.1334481796455131No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT2140.12980868383699912No Hit
AACACACACACTGATTCAGGCTCTG2100.1273823532979898No Hit
GGTATCAACGCAGAGTACATGGGCA2100.1273823532979898No Hit
GAGTAGGGCGGGACACGTGGTATCC2090.12677577066323745No Hit
GTACGGGGCTGTCACCCTGTATCGC2060.12495602275898045No Hit
GTATCGCGCGCCTTTCCAGACGCTT2050.12434944012422813No Hit
GTCAGAGGCGATGAAGGACGTGCTA2020.12252969221997112No Hit
GATCAACGCAGAGTACTTTTTTTTT2000.12131652695046646No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT2000.12131652695046646No Hit
GGGTTTCCCCATTCGGAAATCGCCG1990.12070994431571413No Hit
GCATGTGCATTTTTGTGTACGGGGC1960.11889019641145714No Hit
GTATCAACGCAGAGTACATGGGGAG1960.11889019641145714No Hit
GGGGTTAAGCGACTAAGCGTACACG1930.11707044850720012No Hit
GGATGGTCCCCCCATATTCAGACAG1920.11646386587244781No Hit
AACGCAGAGTACTTTTTTTTTTTTT1920.11646386587244781No Hit
CGGTGGATGCCCTGGCAGTCAGAGG1910.11585728323769548No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT1900.11525070060294314No Hit
ATTCAGACAGGATACCACGTGTCCC1860.11282437006393381No Hit
NGTATCAACGCAGAGTACTTTTTTT1850.11221778742918148No Hit
TATTTAGCCTTGGAGGATGGTCCCC1850.11221778742918148No Hit
GTCTGAATATGGGGGGACCATCCTC1840.11161120479442914No Hit
CACTGATTCAGGCTCTGGGCTGCTC1820.11039803952492448No Hit
ATGATAGTGTGTCGAAACACACTGG1820.11039803952492448No Hit
NATCAACGCAGAGTACTTTTTTTTT1810.10979145689017215No Hit
CACGTGTCCCGCCCTACTCATCGAG1790.10857829162066747No Hit
GGGGAAACCCAGTGTGTTTCGACAC1780.10797170898591515No Hit
GTATCAACGCAGAGTACATGGGGAA1760.10675854371641048No Hit
TAATGATAGTGTGTCGAAACACACT1750.10615196108165814No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC1720.10433221317740116No Hit
GGTATCAACGCAGAGTACATGGGAT1690.10251246527314417No Hit
GTACATGGGGAGCGAACGGGGAGCA1680.10190588263839183No Hit
GAGCAGCCCAGAGCCTGAATCAGTG1670.1012993000036395No Hit
CGTGGTATCCTGTCTGAATATGGGG1660.10069271736888716No Hit
CAACGCAGAGTACTTTTTTTTTTTT1650.10008613473413484No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCAA402.0435409E-417.2771571
CGAATGG551.1399405E-515.51082419
CCGAATG551.1432383E-515.506103518
GGTATCA9900.015.2577491
GATCAAC551.4010954E-414.3602341
AGGGTGA2250.013.9065295
CAGGGTG2400.013.4324424
TACAGGG2600.013.1285412
GTGGTAT3100.012.7389171
ACAGGGT2550.012.64233
CAGCCCC2750.011.7192912
TGGTATC3250.011.6698142
GGGTGAC2700.011.5887756
GACAGCC2700.011.58524510
ATACAGG2400.011.5181041
GGTGACA2650.011.4496297
TGACAGC2650.011.4252689
ACAGCCC2750.011.37460411
GTGACAG2700.011.2034748
AGCCCCG2900.011.1198913
Produced by FastQC (version 0.11.5)