FastQCFastQC Report
Thu 26 May 2016
SRR1512354_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512354_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164858
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69304.203617658833663No Hit
GGTATCAACGCAGAGTACTTTTTTT57663.497555471981948No Hit
TATCAACGCAGAGTACTTTTTTTTT51413.1184413252617404No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25771.5631634497567604No Hit
GTACTGGTTCACTATCGGTCAGTCA18001.091848742554198No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17521.0627327760860863No Hit
ATCATTAACTGAATCCATAGGTTAA17131.0390760533307453No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15950.9674993024299701No Hit
GGATACCACGTGTCCCGCCCTACTC14150.8583144281745503No Hit
ATACAGGGTGACAGCCCCGTACACA11960.7254728311637895No Hit
GGTTAATGAGGCGAACCGGGGGAAC11580.7024226910432008No Hit
GCAGAGTACTTTTTTTTTTTTTTTT10540.6393380970289583No Hit
GTACATGGGGGTTAAGCGACTAAGC10030.6084023826565893No Hit
GTGGATGCCCTGGCAGTCAGAGGCG9840.5968773125962951No Hit
GTGGTATCAACGCAGAGTACTTTTT9070.5501704497203653No Hit
GTGGTATCAACGCAGAGTACATGGG8940.5422848754685852No Hit
GGTATCAACGCAGAGTACATGGGGG8930.5416782928338327No Hit
TATTCAGACAGGATACCACGTGTCC8930.5416782928338327No Hit
TCTAAGTACCCCGAGGAAAAGAAAT8920.5410717101990804No Hit
AGCGTACACGGTGGATGCCCTGGCA8770.5319729706777955No Hit
CGGTACTGGTTCACTATCGGTCAGT7480.4537238107947446No Hit
GTATCAACGCAGAGTACATGGGGGT7480.4537238107947446No Hit
ATCAACGCAGAGTACTTTTTTTTTT7200.43673949702167925No Hit
CTATCGGTCAGTCAGGAGTATTTAG7080.4294605054046513No Hit
CCCATATTCAGACAGGATACCACGT7070.4288539227698989No Hit
GTATTTAGCCTTGGAGGATGGTCCC7050.4276407575003943No Hit
TCACGGTACTGGTTCACTATCGGTC6000.3639495808513994No Hit
ACTTAGATGTTTCAGTTCCCCCGGT5930.35970350240813304No Hit
GTTATAACCGGCGATTTCCGAATGG5920.35909691977338076No Hit
GTCAGGAGTATTTAGCCTTGGAGGA5870.35606400659961907No Hit
ACCCTGTATCGCGCGCCTTTCCAGA5740.34817843234783874No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG5740.34817843234783874No Hit
CATCTAAGTACCCCGAGGAAAAGAA5700.3457521018088294No Hit
GGTTCACTATCGGTCAGTCAGGAGT5690.3451455191740771No Hit
GTTCACTATCGGTCAGTCAGGAGTA5680.34453893653932477No Hit
ACGCAGAGTACATGGGGGTTAAGCG5640.34211260600031546No Hit
GTTAATGATAGTGTGTCGAAACACA5640.34211260600031546No Hit
TATCAACGCAGAGTACATGGGGGTT5530.3354401970180398No Hit
GTGCTAATCTGCGATAAGCGTCGGT4920.29843865629814753No Hit
GATATGAACCGTTATAACCGGCGAT4920.29843865629814753No Hit
GAGTACATGGGGGTTAAGCGACTAA4910.2978320736633952No Hit
GGAGTATTTAGCCTTGGAGGATGGT4790.29055308204636715No Hit
GGTGATATGAACCGTTATAACCGGC4780.2899464994116148No Hit
GAATCAGTGTGTGTGTTAGTGGAAG4570.27720826408181587No Hit
GCCCAGAGCCTGAATCAGTGTGTGT4540.2753885161775589No Hit
GCCTGAATCAGTGTGTGTGTTAGTG4510.27356876827330184No Hit
GCCTTGGAGGATGGTCCCCCCATAT4500.2729621856385495No Hit
CACTATCATTAACTGAATCCATAGG4490.2723556030037972No Hit
GGTATCAACGCAGAGTACATGGGGA4340.2632568634825122No Hit
TCGGTAAGGTGATATGAACCGTTAT4180.2535515413264749No Hit
ATCCATAGGTTAATGAGGCGAACCG4010.24323963653568528No Hit
CAGTAGCGGCGAGCGAACGGGGAGC3940.2389935580924189No Hit
CACACACACTGATTCAGGCTCTGGG3890.23596064491865726No Hit
CCGTTATAACCGGCGATTTCCGAAT3830.2323211491101433No Hit
CTATCATTAACTGAATCCATAGGTT3800.23050140120588627No Hit
TCATTAACCTATGGATTCAGTTAAT3750.22746848803212463No Hit
TAAGTACCCCGAGGAAAAGAAATCA3620.2195829137803443No Hit
GGGAAACCCAGTGTGTTTCGACACA3570.21655000060658267No Hit
GGGTGACAGCCCCGTACACAAAAAT3470.21048417425905933No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG3320.20138543473777432No Hit
GCGTACACGGTGGATGCCCTGGCAG3220.19531960839025098No Hit
AGCTCACAGCATGTGCATTTTTGTG3130.18986036467748002No Hit
TTTGTGTACGGGGCTGTCACCCTGT3110.18864719940797534No Hit
CCCCAGTAGCGGCGAGCGAACGGGG3100.188040616773223No Hit
GGGTACTTAGATGTTTCAGTTCCCC3060.18561428623421367No Hit
AGTCAGGAGTATTTAGCCTTGGAGG3030.18379453832995668No Hit
GTACTTAGATGTTTCAGTTCCCCCG2960.17954845988669038No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA2940.1783352946171857No Hit
AAGTACCCCGAGGAAAAGAAATCAA2940.1783352946171857No Hit
GGTATCAACGCAGAGTACATGGGCA2870.17408921617391937No Hit
GTGTTTCGACACACTATCATTAACT2850.1728760509044147No Hit
TCCCCCAGTAGCGGCGAGCGAACGG2830.17166288563491003No Hit
GATTCAGGCTCTGGGCTGCTCCCCG2810.17044972036540537No Hit
GGGGAAACCCAGTGTGTTTCGACAC2800.16984313773065304No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA2760.16741680719164373No Hit
CATTAACTGAATCCATAGGTTAATG2750.16681022455689137No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT2740.16620364192213904No Hit
AGGTTAATGAGGCGAACCGGGGGAA2670.16195756347887272No Hit
GGAGGATGGTCCCCCCATATTCAGA2650.16074439820936806No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA2640.16013781557461573No Hit
TTCCAGACGCTTCCACTAACACACA2600.15771148503560642No Hit
GTGGTATCCTGTCTGAATATGGGGG2590.15710490240085406No Hit
ATTTAGCCTTGGAGGATGGTCCCCC2550.15467857186184475No Hit
CCTATGGATTCAGTTAATGATAGTG2540.15407198922709242No Hit
CAACGCAGAGTACTTTTTTTTTTTT2530.15346540659234006No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT2520.15285882395758774No Hit
TCAACGCAGAGTACTTTTTTTTTTT2490.15103907605333075No Hit
AACGCAGAGTACTTTTTTTTTTTTT2470.14982591078382607No Hit
GAATATGGGGGGACCATCCTCCAAG2410.1461864149753121No Hit
GGGGTTAAGCGACTAAGCGTACACG2360.14315350180155043No Hit
GTACATGGGCATCTAAGTACCCCGA2360.14315350180155043No Hit
GGGTTTCCCCATTCGGAAATCGCCG2300.13951400599303643No Hit
CGGTGGATGCCCTGGCAGTCAGAGG2300.13951400599303643No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT2280.13830084072353177No Hit
AACACACACACTGATTCAGGCTCTG2260.1370876754540271No Hit
GTGCATTTTTGTGTACGGGGCTGTC2130.1292021012022468No Hit
CCACGTGTCCCGCCCTACTCATCGA2120.12859551856749446No Hit
GTATCGCGCGCCTTTCCAGACGCTT2110.1279889359327421No Hit
TAATGATAGTGTGTCGAAACACACT2080.12616918802848512No Hit
CCCTGGCAGTCAGAGGCGATGAAGG2060.12495602275898045No Hit
AATCTGCGATAAGCGTCGGTAAGGT2010.1219231095852188No Hit
GAGGAAAAGAAATCAACCGAGATTC2000.12131652695046646No Hit
ACCCAGTGTGTTTCGACACACTATC1980.12010336168096179No Hit
AAAATGCACATGCTGTGAGCTCGAT1970.11949677904620945No Hit
ATGATAGTGTGTCGAAACACACTGG1970.11949677904620945No Hit
TATTTAGCCTTGGAGGATGGTCCCC1950.11828361377670481No Hit
GAGCAGCCCAGAGCCTGAATCAGTG1940.11767703114195247No Hit
GGGCTGTCACCCTGTATCGCGCGCC1940.11767703114195247No Hit
CAGGGTGACAGCCCCGTACACAAAA1930.11707044850720012No Hit
GGTATCAACGCAGAGTACATGGGCG1920.11646386587244781No Hit
CATTAACCTATGGATTCAGTTAATG1910.11585728323769548No Hit
GAGTAGGGCGGGACACGTGGTATCC1900.11525070060294314No Hit
CACTGATTCAGGCTCTGGGCTGCTC1880.11403753533343847No Hit
GCGATAAGCGTCGGTAAGGTGATAT1860.11282437006393381No Hit
GTACGGGGCTGTCACCCTGTATCGC1860.11282437006393381No Hit
GTATCAACGCAGAGTACATGGGGAG1820.11039803952492448No Hit
ATTCAGACAGGATACCACGTGTCCC1810.10979145689017215No Hit
GGTATCAACGCAGAGTACATGGGAT1790.10857829162066747No Hit
CACGTGTCCCGCCCTACTCATCGAG1790.10857829162066747No Hit
AGATTCCCCCAGTAGCGGCGAGCGA1790.10857829162066747No Hit
CGTGGTATCCTGTCTGAATATGGGG1770.10736512635116283No Hit
GCCCTACTCATCGAGCTCACAGCAT1740.10554537844690583No Hit
CCCAGTGTGTTTCGACACACTATCA1730.1049387958121535No Hit
ACCGAGATTCCCCCAGTAGCGGCGA1700.10311904790789649No Hit
CTAACACACACACTGATTCAGGCTC1690.10251246527314417No Hit
AACGGGGAGCAGCCCAGAGCCTGAA1680.10190588263839183No Hit
GACTAAGCGTACACGGTGGATGCCC1680.10190588263839183No Hit
CGTCTGGAAAGGCGCGCGATACAGG1670.1012993000036395No Hit
CCATAGGTTAATGAGGCGAACCGGG1670.1012993000036395No Hit
GTAGCGGCGAGCGAACGGGGAGCAG1670.1012993000036395No Hit
GGTATCAACGCAGAGTACATGGGAA1650.10008613473413484No Hit
ATGAAGGACGTGCTAATCTGCGATA1650.10008613473413484No Hit
GTATCAACGCAGAGTACATGGGCGA1650.10008613473413484No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCAC250.00600473819.002733
GAAATCG350.002161456716.28311316
ATCGCCG350.002161456716.28311319
AATCGCC350.002161456716.28311318
GGTATCA12350.015.0974111
CGAATGG1058.54925E-1114.47387919
CCGAATG1058.54925E-1114.47387918
AAATCGC400.00525822914.24772517
GTGGTAT3300.012.972151
TGGTATC3400.012.858692
TTTCCGA1209.760697E-911.87310415
TTCCGAA1209.760697E-911.87310416
TCCGAAT1251.7858838E-811.39818117
ATTTCCG1251.7858838E-811.39818114
TTAACTG3100.011.340345
ACTGAAT3150.011.1569488
TAACTGA3100.011.0304986
TGAATCC3100.011.03049810
GATTTCC1303.1846866E-810.95978813
ACCGGGG2100.010.8554115
Produced by FastQC (version 0.11.5)