##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512354_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 164858 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.594535903626152 31.0 31.0 34.0 27.0 34.0 2 30.65535187858642 31.0 31.0 34.0 27.0 34.0 3 30.636820779094737 31.0 31.0 34.0 27.0 34.0 4 34.342506884712904 37.0 35.0 37.0 31.0 37.0 5 34.09580972715913 37.0 35.0 37.0 30.0 37.0 6 34.184467845054535 37.0 35.0 37.0 30.0 37.0 7 34.08847614310498 37.0 35.0 37.0 30.0 37.0 8 34.049697315265256 37.0 35.0 37.0 30.0 37.0 9 35.36004925450994 39.0 35.0 39.0 30.0 39.0 10 35.279907556806464 39.0 35.0 39.0 30.0 39.0 11 35.37950235960645 39.0 35.0 39.0 30.0 39.0 12 35.184467845054535 39.0 35.0 39.0 29.0 39.0 13 35.1462228099334 39.0 35.0 39.0 29.0 39.0 14 35.87890790862439 39.0 36.0 41.0 27.0 41.0 15 35.734577636511425 39.0 36.0 41.0 27.0 41.0 16 35.58674131676958 39.0 35.0 41.0 27.0 41.0 17 35.849925390335926 39.0 36.0 41.0 27.0 41.0 18 35.882972012277236 39.0 36.0 41.0 27.0 41.0 19 35.92300646617089 40.0 36.0 41.0 27.0 41.0 20 35.99300003639496 40.0 36.0 41.0 28.0 41.0 21 35.907186791056546 40.0 36.0 41.0 27.0 41.0 22 35.78122990695022 40.0 36.0 41.0 27.0 41.0 23 35.60156013053658 40.0 35.0 41.0 27.0 41.0 24 35.37008213128875 39.0 35.0 41.0 26.0 41.0 25 35.17973043467712 39.0 35.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.038522890347881145 1101 2 -0.08100276585355459 1101 3 -0.08786776341504066 1101 4 0.11199737982857982 1101 5 -0.36831850213432205 1101 6 0.17944224504628892 1101 7 -0.04154141035940029 1101 8 -0.1628564933369887 1101 9 -0.16403032098702397 1101 10 0.13351238852147418 1101 11 0.07306424274647583 1101 12 0.11834102770633592 1101 13 0.2498204375771209 1101 14 -0.021563654770119456 1101 15 0.48354900083093355 1101 16 0.4436572417110227 1101 17 0.23839900373238976 1101 18 -0.7523139960547667 1101 19 -0.3990956523923188 1101 20 -0.11019815063709615 1101 21 -0.46066691466733545 1101 22 -0.20384474991448087 1101 23 0.10096668355578942 1101 24 -0.019464205999049966 1101 25 0.18116249953247632 1102 1 1.6118636600076712 1102 2 1.5236077565483797 1102 3 1.3468970627608314 1102 4 1.645144805000541 1102 5 1.7247471234466403 1102 6 1.579999969530391 1102 7 1.6716022966493327 1102 8 1.6013004131512005 1102 9 2.1115599374253193 1102 10 2.0059793898228264 1102 11 1.8784183977938866 1102 12 1.422226508278932 1102 13 1.8978962881069563 1102 14 1.9237421641553354 1102 15 2.3467949573284272 1102 16 2.460277431337353 1102 17 2.3022398663462553 1102 18 2.129188141889088 1102 19 2.289991538535361 1102 20 2.192757789128663 1102 21 2.0234007559034026 1102 22 2.2055622030507536 1102 23 2.157445582979477 1102 24 2.2893664316659397 1102 25 2.775213438368695 1103 1 0.4258415895925367 1103 2 0.3976312282597121 1103 3 0.5230049625681517 1103 4 0.5504171819492214 1103 5 0.4974568799714234 1103 6 0.5751654976439013 1103 7 0.16275661439325262 1103 8 0.6141923381818586 1103 9 0.5779463448521867 1103 10 0.6582827704499081 1103 11 0.6881924387817975 1103 12 0.27270820384732986 1103 13 0.8663671236298143 1103 14 1.0759929961749535 1103 15 0.7157912789258987 1103 16 0.5518171055359815 1103 17 0.4098944468332064 1103 18 0.18548521668327567 1103 19 1.0546438419159898 1103 20 0.9819327759915097 1103 21 0.7205806473029455 1103 22 1.1704947656711795 1103 23 1.0639440941022613 1103 24 1.175826407143255 1103 25 1.0198447223063383 1104 1 0.9868636600076712 1104 2 0.6175471504877699 1104 3 0.9052303960941614 1104 4 0.4887054110611473 1104 5 0.5595956082951261 1104 6 0.6288636058940327 1104 7 0.7814507814978242 1104 8 1.158497382848175 1104 9 1.3392114525768335 1104 10 0.9415854504288887 1104 11 1.4409183977938866 1104 12 1.3025295385819646 1104 13 0.9717599244705966 1104 14 1.2786664065795748 1104 15 1.7979313209647856 1104 16 1.8034592495191717 1104 17 1.5564065330129253 1104 18 1.5738851115860584 1104 19 1.2687794173232447 1104 20 1.7245759709468444 1104 21 1.6453704528731024 1104 22 1.3960925060810538 1104 23 1.209339522373412 1104 24 1.3851997649992782 1104 25 1.4176376807929358 1105 1 0.5632743183148818 1105 2 0.6556871713864147 1105 3 0.7514947639102552 1105 4 0.4874645542168423 1105 5 -0.21914002388878373 1105 6 -0.010177668923105898 1105 7 -0.11523155915003969 1105 8 0.11572047584712664 1105 9 0.026188986537128756 1105 10 -0.048879544346448256 1105 11 0.12397743645637149 1105 12 0.32107708299157167 1105 13 0.30228500284049886 1105 14 0.4719135330163553 1105 15 0.7185817911842207 1105 16 0.6194205870322307 1105 17 0.40182189351448727 1105 18 0.25896870615240886 1105 19 0.668988403739128 1105 20 0.5963105581986738 1105 21 0.4404331487978652 1105 22 0.5262518582231692 1105 23 0.5420850814120755 1105 24 0.936074895615782 1105 25 0.7995603558190609 1106 1 0.37738775340061537 1106 2 0.4523707967868269 1106 3 0.384055532706185 1106 4 0.104856677827172 1106 5 0.19210926949731544 1106 6 0.43037254777182454 1106 7 0.7600275326155526 1106 8 0.9183396580593026 1106 9 0.6705266537690875 1106 10 0.9399088483424549 1106 11 0.8918312144853857 1106 12 0.617854923579479 1106 13 0.7980453194035277 1106 14 0.7803740568527999 1106 15 0.20134041187387908 1106 16 0.8448775306915479 1106 17 0.977947765004977 1106 18 0.8131424389581383 1106 19 1.070816178376397 1106 20 0.9710985740268185 1106 21 0.849232847309267 1106 22 1.1596605637064883 1106 23 0.621131623714696 1106 24 1.2896644942590854 1106 25 1.6029332595311416 1107 1 1.129834061699004 1107 2 1.2761095183524667 1107 3 1.0593001635360224 1107 4 0.8989855379110452 1107 5 0.6584328175974505 1107 6 0.6963847475430782 1107 7 0.8916516271637818 1107 8 1.2347394547297768 1107 9 1.827979951519751 1107 10 1.7457609261159917 1107 11 1.5031541271807782 1107 12 1.8807536400618758 1107 13 1.3497990365213326 1107 14 1.9952361740214357 1107 15 1.6955264583855083 1107 16 1.7392414905339706 1107 17 1.8889910996091146 1107 18 1.1809147098947292 1107 19 1.6308301572809611 1107 20 2.017387810270307 1107 21 2.0853810236976287 1107 22 1.4733916604150963 1107 23 1.7430340255446595 1107 24 1.7265052618280308 1107 25 1.4636207674736994 1108 1 0.6009164919924395 1108 2 0.7391559082221839 1108 3 0.6938429615391861 1108 4 0.9683877052067942 1108 5 0.9928416815935535 1108 6 0.4689100123671395 1108 7 0.3320814335682627 1108 8 0.21516682597049197 1108 9 0.6873492441046807 1108 10 0.2664902576635413 1108 11 0.4153055943669415 1108 12 0.5580031225895752 1108 13 0.9621514047180924 1108 14 0.845747558412036 1108 15 0.625424181507384 1108 16 1.0739851895477273 1108 17 1.136160221732581 1108 18 0.8354867298630708 1108 19 1.2385557143246686 1108 20 0.9569653093571233 1108 21 0.46706250427719453 1108 22 0.22929693254464922 1108 23 1.1452926399364785 1108 24 1.4642990034571497 1108 25 1.700836157708693 1109 1 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2315 24 -2.4774445351450254 2315 25 -2.505402242246987 2316 1 -1.2054645516867382 2316 2 -1.4658928836564868 2316 3 -1.2315066931077148 2316 4 -1.173293095127498 2316 5 -1.3875874902964256 2316 6 -1.6454375037944047 2316 7 -1.822209056316943 2316 8 -1.8757802526618548 2316 9 -2.10858517567754 2316 10 -2.4255186895625727 2316 11 -2.325593552696944 2316 12 -2.550543444772714 2316 13 -2.3684289359647437 2316 14 -3.141814814077705 2316 15 -3.202415456670721 2316 16 -3.065458804258043 2316 17 -2.877358938604665 2316 18 -3.03308245136742 2316 19 -2.7630174243327517 2316 20 -3.111638284281497 2316 21 -2.9358822146471724 2316 22 -3.1906819284046435 2316 23 -2.828725991924493 2316 24 -3.123096223050233 2316 25 -2.750949600470399 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3276.0 3 0.0 4 56.0 5 479.0 6 564.0 7 455.0 8 351.0 9 351.0 10 284.0 11 294.0 12 268.0 13 268.0 14 284.0 15 232.0 16 253.0 17 275.0 18 299.0 19 316.0 20 430.0 21 535.0 22 655.0 23 800.0 24 1016.0 25 1102.0 26 1335.0 27 1491.0 28 1772.0 29 2101.0 30 2726.0 31 3227.0 32 4371.0 33 5478.0 34 7327.0 35 10621.0 36 17151.0 37 38571.0 38 55844.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.31970508569277 18.576686222351785 14.067946895990477 16.03566179596497 2 20.912824308011476 22.966800322715663 36.011913788815356 20.108461580457504 3 23.72647975833748 29.365878513629912 28.586419827973163 18.321221900059445 4 14.716301301726334 19.687852576156452 33.62651494013029 31.96933118198692 5 13.148247062251142 39.53275225793921 29.484315820141788 17.834684859667867 6 26.760178854192578 35.97044173588065 18.606781655918024 18.662597754008747 7 24.61930473821513 36.53885821755748 19.162773766911364 19.679063277316022 8 29.228267925283763 29.042629992052753 20.386199700310005 21.342902382353476 9 29.94011612738823 11.91367500106177 19.19317554408169 38.95303332746831 10 23.42848475655999 27.343546185348096 23.676020021234642 25.551949036857273 11 34.83882319392789 23.807934764802603 18.317062959974276 23.036179081295238 12 26.46050611868489 29.086959950977715 25.466712776736255 18.985821153601133 13 35.72351617150103 20.95409125932038 25.031093206816845 18.291299362361748 14 24.70730967546254 26.069760388231728 29.269639065817408 19.95329087048832 15 29.853498741241776 24.723831478055143 27.72968546210076 17.692984318602324 16 20.93875297985527 27.811982506050697 26.47203333798383 24.777231176110202 17 20.54313409115724 27.169442793191717 26.695095172815392 25.592327942835652 18 20.002790297045316 19.958509496108746 34.99821057037311 25.040489636472824 19 21.756299360661906 24.159579759550642 35.30068301204673 18.78343786774072 20 25.020927100898955 17.353116014994725 37.16228511810164 20.463671766004683 21 26.834609178808428 20.937412803746255 35.37347292821701 16.854505089228304 22 24.954504646592095 21.13350156504016 35.040521194768644 18.871472593599105 23 25.41717973759076 21.247505413782854 35.43252637134001 17.902788477286375 24 23.52994698467772 21.10543619357265 34.68196872459936 20.682648097150274 25 22.214875832534666 22.623105945722983 36.453190018076164 18.708828203666187 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 544.0 1 544.0 2 572.5 3 601.0 4 601.0 5 601.0 6 1234.0 7 1867.0 8 1867.0 9 1867.0 10 1816.0 11 1765.0 12 1765.0 13 1765.0 14 1027.5 15 290.0 16 290.0 17 290.0 18 910.0 19 1530.0 20 1530.0 21 1530.0 22 2699.5 23 3869.0 24 3869.0 25 3869.0 26 6889.5 27 9910.0 28 9910.0 29 9910.0 30 10196.0 31 10482.0 32 10482.0 33 10482.0 34 11038.5 35 11595.0 36 11595.0 37 11595.0 38 9746.5 39 7898.0 40 7898.0 41 7898.0 42 10077.5 43 12257.0 44 12257.0 45 12257.0 46 19113.0 47 25969.0 48 25969.0 49 25969.0 50 25687.0 51 25405.0 52 25405.0 53 25405.0 54 21809.0 55 18213.0 56 18213.0 57 18213.0 58 14986.0 59 11759.0 60 11759.0 61 11759.0 62 11687.0 63 11615.0 64 11615.0 65 11615.0 66 8272.5 67 4930.0 68 4930.0 69 4930.0 70 3947.5 71 2965.0 72 2965.0 73 2965.0 74 2003.0 75 1041.0 76 1041.0 77 1041.0 78 667.5 79 294.0 80 294.0 81 294.0 82 165.5 83 37.0 84 37.0 85 37.0 86 26.5 87 16.0 88 16.0 89 16.0 90 8.0 91 0.0 92 0.0 93 0.0 94 2.5 95 5.0 96 5.0 97 5.0 98 3.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12131652695046646 2 0.004246078443266326 3 0.0 4 0.0 5 0.06429775928374723 6 0.018804061677322303 7 0.016984313773065304 8 0.012738235329798979 9 0.02365672275534096 10 0.020017226946826967 11 0.02365672275534096 12 0.02123039221633163 13 0.018804061677322303 14 0.004852661078018658 15 0.00788557425178032 16 6.065826347523322E-4 17 0.0 18 6.065826347523322E-4 19 0.0 20 0.0 21 0.0 22 0.003639495808513994 23 6.065826347523322E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 164858.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.17812905652137 #Duplication Level Percentage of deduplicated Percentage of total 1 80.5291669937976 18.665154253964015 2 9.544372039465076 4.424413737883512 3 3.1875637905315224 2.2164529473850223 4 1.4498442856768994 1.3441871186111685 5 0.7903483290152051 0.9159397784760218 6 0.60715500772029 0.8443630275752466 7 0.41611054408416426 0.6751264724793459 8 0.31142864620135563 0.5774666682842203 9 0.2721729344953024 0.567761346128183 >10 1.8738059721022742 9.201251986558129 >50 0.42919578131951536 7.05880212061289 >100 0.4893878726021303 22.76504628225503 >500 0.06542618617675539 10.666149049485012 >1k 0.026170474470702157 9.258270754224847 >5k 0.007851142341210647 10.819614456077352 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6930 4.203617658833663 No Hit GGTATCAACGCAGAGTACTTTTTTT 5766 3.497555471981948 No Hit TATCAACGCAGAGTACTTTTTTTTT 5141 3.1184413252617404 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2577 1.5631634497567604 No Hit GTACTGGTTCACTATCGGTCAGTCA 1800 1.091848742554198 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1752 1.0627327760860863 No Hit ATCATTAACTGAATCCATAGGTTAA 1713 1.0390760533307453 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1595 0.9674993024299701 No Hit GGATACCACGTGTCCCGCCCTACTC 1415 0.8583144281745503 No Hit ATACAGGGTGACAGCCCCGTACACA 1196 0.7254728311637895 No Hit GGTTAATGAGGCGAACCGGGGGAAC 1158 0.7024226910432008 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 1054 0.6393380970289583 No Hit GTACATGGGGGTTAAGCGACTAAGC 1003 0.6084023826565893 No Hit GTGGATGCCCTGGCAGTCAGAGGCG 984 0.5968773125962951 No Hit GTGGTATCAACGCAGAGTACTTTTT 907 0.5501704497203653 No Hit GTGGTATCAACGCAGAGTACATGGG 894 0.5422848754685852 No Hit GGTATCAACGCAGAGTACATGGGGG 893 0.5416782928338327 No Hit TATTCAGACAGGATACCACGTGTCC 893 0.5416782928338327 No Hit TCTAAGTACCCCGAGGAAAAGAAAT 892 0.5410717101990804 No Hit AGCGTACACGGTGGATGCCCTGGCA 877 0.5319729706777955 No Hit CGGTACTGGTTCACTATCGGTCAGT 748 0.4537238107947446 No Hit GTATCAACGCAGAGTACATGGGGGT 748 0.4537238107947446 No Hit ATCAACGCAGAGTACTTTTTTTTTT 720 0.43673949702167925 No Hit CTATCGGTCAGTCAGGAGTATTTAG 708 0.4294605054046513 No Hit CCCATATTCAGACAGGATACCACGT 707 0.4288539227698989 No Hit GTATTTAGCCTTGGAGGATGGTCCC 705 0.4276407575003943 No Hit TCACGGTACTGGTTCACTATCGGTC 600 0.3639495808513994 No Hit ACTTAGATGTTTCAGTTCCCCCGGT 593 0.35970350240813304 No Hit GTTATAACCGGCGATTTCCGAATGG 592 0.35909691977338076 No Hit GTCAGGAGTATTTAGCCTTGGAGGA 587 0.35606400659961907 No Hit ACCCTGTATCGCGCGCCTTTCCAGA 574 0.34817843234783874 No Hit GTGTGTGTGTTAGTGGAAGCGTCTG 574 0.34817843234783874 No Hit CATCTAAGTACCCCGAGGAAAAGAA 570 0.3457521018088294 No Hit GGTTCACTATCGGTCAGTCAGGAGT 569 0.3451455191740771 No Hit GTTCACTATCGGTCAGTCAGGAGTA 568 0.34453893653932477 No Hit ACGCAGAGTACATGGGGGTTAAGCG 564 0.34211260600031546 No Hit GTTAATGATAGTGTGTCGAAACACA 564 0.34211260600031546 No Hit TATCAACGCAGAGTACATGGGGGTT 553 0.3354401970180398 No Hit GTGCTAATCTGCGATAAGCGTCGGT 492 0.29843865629814753 No Hit GATATGAACCGTTATAACCGGCGAT 492 0.29843865629814753 No Hit GAGTACATGGGGGTTAAGCGACTAA 491 0.2978320736633952 No Hit GGAGTATTTAGCCTTGGAGGATGGT 479 0.29055308204636715 No Hit GGTGATATGAACCGTTATAACCGGC 478 0.2899464994116148 No Hit GAATCAGTGTGTGTGTTAGTGGAAG 457 0.27720826408181587 No Hit GCCCAGAGCCTGAATCAGTGTGTGT 454 0.2753885161775589 No Hit GCCTGAATCAGTGTGTGTGTTAGTG 451 0.27356876827330184 No Hit GCCTTGGAGGATGGTCCCCCCATAT 450 0.2729621856385495 No Hit CACTATCATTAACTGAATCCATAGG 449 0.2723556030037972 No Hit GGTATCAACGCAGAGTACATGGGGA 434 0.2632568634825122 No Hit TCGGTAAGGTGATATGAACCGTTAT 418 0.2535515413264749 No Hit ATCCATAGGTTAATGAGGCGAACCG 401 0.24323963653568528 No Hit CAGTAGCGGCGAGCGAACGGGGAGC 394 0.2389935580924189 No Hit CACACACACTGATTCAGGCTCTGGG 389 0.23596064491865726 No Hit CCGTTATAACCGGCGATTTCCGAAT 383 0.2323211491101433 No Hit CTATCATTAACTGAATCCATAGGTT 380 0.23050140120588627 No Hit TCATTAACCTATGGATTCAGTTAAT 375 0.22746848803212463 No Hit TAAGTACCCCGAGGAAAAGAAATCA 362 0.2195829137803443 No Hit GGGAAACCCAGTGTGTTTCGACACA 357 0.21655000060658267 No Hit GGGTGACAGCCCCGTACACAAAAAT 347 0.21048417425905933 No Hit GTGTTAGTGGAAGCGTCTGGAAAGG 332 0.20138543473777432 No Hit GCGTACACGGTGGATGCCCTGGCAG 322 0.19531960839025098 No Hit AGCTCACAGCATGTGCATTTTTGTG 313 0.18986036467748002 No Hit TTTGTGTACGGGGCTGTCACCCTGT 311 0.18864719940797534 No Hit CCCCAGTAGCGGCGAGCGAACGGGG 310 0.188040616773223 No Hit GGGTACTTAGATGTTTCAGTTCCCC 306 0.18561428623421367 No Hit AGTCAGGAGTATTTAGCCTTGGAGG 303 0.18379453832995668 No Hit GTACTTAGATGTTTCAGTTCCCCCG 296 0.17954845988669038 No Hit GTGTGTGTTAGTGGAAGCGTCTGGA 294 0.1783352946171857 No Hit AAGTACCCCGAGGAAAAGAAATCAA 294 0.1783352946171857 No Hit GGTATCAACGCAGAGTACATGGGCA 287 0.17408921617391937 No Hit GTGTTTCGACACACTATCATTAACT 285 0.1728760509044147 No Hit TCCCCCAGTAGCGGCGAGCGAACGG 283 0.17166288563491003 No Hit GATTCAGGCTCTGGGCTGCTCCCCG 281 0.17044972036540537 No Hit GGGGAAACCCAGTGTGTTTCGACAC 280 0.16984313773065304 No Hit GTGTGTTAGTGGAAGCGTCTGGAAA 276 0.16741680719164373 No Hit CATTAACTGAATCCATAGGTTAATG 275 0.16681022455689137 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 274 0.16620364192213904 No Hit AGGTTAATGAGGCGAACCGGGGGAA 267 0.16195756347887272 No Hit GGAGGATGGTCCCCCCATATTCAGA 265 0.16074439820936806 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 264 0.16013781557461573 No Hit TTCCAGACGCTTCCACTAACACACA 260 0.15771148503560642 No Hit GTGGTATCCTGTCTGAATATGGGGG 259 0.15710490240085406 No Hit ATTTAGCCTTGGAGGATGGTCCCCC 255 0.15467857186184475 No Hit CCTATGGATTCAGTTAATGATAGTG 254 0.15407198922709242 No Hit CAACGCAGAGTACTTTTTTTTTTTT 253 0.15346540659234006 No Hit TTTTTTTTTTTTTTTTTTTTTTTTT 252 0.15285882395758774 No Hit TCAACGCAGAGTACTTTTTTTTTTT 249 0.15103907605333075 No Hit AACGCAGAGTACTTTTTTTTTTTTT 247 0.14982591078382607 No Hit GAATATGGGGGGACCATCCTCCAAG 241 0.1461864149753121 No Hit GGGGTTAAGCGACTAAGCGTACACG 236 0.14315350180155043 No Hit GTACATGGGCATCTAAGTACCCCGA 236 0.14315350180155043 No Hit GGGTTTCCCCATTCGGAAATCGCCG 230 0.13951400599303643 No Hit CGGTGGATGCCCTGGCAGTCAGAGG 230 0.13951400599303643 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 228 0.13830084072353177 No Hit AACACACACACTGATTCAGGCTCTG 226 0.1370876754540271 No Hit GTGCATTTTTGTGTACGGGGCTGTC 213 0.1292021012022468 No Hit CCACGTGTCCCGCCCTACTCATCGA 212 0.12859551856749446 No Hit GTATCGCGCGCCTTTCCAGACGCTT 211 0.1279889359327421 No Hit TAATGATAGTGTGTCGAAACACACT 208 0.12616918802848512 No Hit CCCTGGCAGTCAGAGGCGATGAAGG 206 0.12495602275898045 No Hit AATCTGCGATAAGCGTCGGTAAGGT 201 0.1219231095852188 No Hit GAGGAAAAGAAATCAACCGAGATTC 200 0.12131652695046646 No Hit ACCCAGTGTGTTTCGACACACTATC 198 0.12010336168096179 No Hit AAAATGCACATGCTGTGAGCTCGAT 197 0.11949677904620945 No Hit ATGATAGTGTGTCGAAACACACTGG 197 0.11949677904620945 No Hit TATTTAGCCTTGGAGGATGGTCCCC 195 0.11828361377670481 No Hit GAGCAGCCCAGAGCCTGAATCAGTG 194 0.11767703114195247 No Hit GGGCTGTCACCCTGTATCGCGCGCC 194 0.11767703114195247 No Hit CAGGGTGACAGCCCCGTACACAAAA 193 0.11707044850720012 No Hit GGTATCAACGCAGAGTACATGGGCG 192 0.11646386587244781 No Hit CATTAACCTATGGATTCAGTTAATG 191 0.11585728323769548 No Hit GAGTAGGGCGGGACACGTGGTATCC 190 0.11525070060294314 No Hit CACTGATTCAGGCTCTGGGCTGCTC 188 0.11403753533343847 No Hit GCGATAAGCGTCGGTAAGGTGATAT 186 0.11282437006393381 No Hit GTACGGGGCTGTCACCCTGTATCGC 186 0.11282437006393381 No Hit GTATCAACGCAGAGTACATGGGGAG 182 0.11039803952492448 No Hit ATTCAGACAGGATACCACGTGTCCC 181 0.10979145689017215 No Hit GGTATCAACGCAGAGTACATGGGAT 179 0.10857829162066747 No Hit CACGTGTCCCGCCCTACTCATCGAG 179 0.10857829162066747 No Hit AGATTCCCCCAGTAGCGGCGAGCGA 179 0.10857829162066747 No Hit CGTGGTATCCTGTCTGAATATGGGG 177 0.10736512635116283 No Hit GCCCTACTCATCGAGCTCACAGCAT 174 0.10554537844690583 No Hit CCCAGTGTGTTTCGACACACTATCA 173 0.1049387958121535 No Hit ACCGAGATTCCCCCAGTAGCGGCGA 170 0.10311904790789649 No Hit CTAACACACACACTGATTCAGGCTC 169 0.10251246527314417 No Hit AACGGGGAGCAGCCCAGAGCCTGAA 168 0.10190588263839183 No Hit GACTAAGCGTACACGGTGGATGCCC 168 0.10190588263839183 No Hit CGTCTGGAAAGGCGCGCGATACAGG 167 0.1012993000036395 No Hit CCATAGGTTAATGAGGCGAACCGGG 167 0.1012993000036395 No Hit GTAGCGGCGAGCGAACGGGGAGCAG 167 0.1012993000036395 No Hit GGTATCAACGCAGAGTACATGGGAA 165 0.10008613473413484 No Hit ATGAAGGACGTGCTAATCTGCGATA 165 0.10008613473413484 No Hit GTATCAACGCAGAGTACATGGGCGA 165 0.10008613473413484 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAGCAC 25 0.006004738 19.00273 3 GAAATCG 35 0.0021614567 16.283113 16 ATCGCCG 35 0.0021614567 16.283113 19 AATCGCC 35 0.0021614567 16.283113 18 GGTATCA 1235 0.0 15.097411 1 CGAATGG 105 8.54925E-11 14.473879 19 CCGAATG 105 8.54925E-11 14.473879 18 AAATCGC 40 0.005258229 14.247725 17 GTGGTAT 330 0.0 12.97215 1 TGGTATC 340 0.0 12.85869 2 TTTCCGA 120 9.760697E-9 11.873104 15 TTCCGAA 120 9.760697E-9 11.873104 16 TCCGAAT 125 1.7858838E-8 11.398181 17 ATTTCCG 125 1.7858838E-8 11.398181 14 TTAACTG 310 0.0 11.34034 5 ACTGAAT 315 0.0 11.156948 8 TAACTGA 310 0.0 11.030498 6 TGAATCC 310 0.0 11.030498 10 GATTTCC 130 3.1846866E-8 10.959788 13 ACCGGGG 210 0.0 10.85541 15 >>END_MODULE