FastQCFastQC Report
Thu 26 May 2016
SRR1512353_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512353_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1485506
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT78160.526150685355697No Hit
GGTATCAACGCAGAGTACTTTTTTT53340.3590695695608096No Hit
TATCAACGCAGAGTACTTTTTTTTT46820.3151788010280672No Hit
CTGTAGGACGTGGAATATGGCAAGA32840.2210694537753466No Hit
GTCCTACAGTGGACATTTCTAAATT31830.21427042368055058No Hit
GTCCTAAAGTGTGTATTTCTCATTT31290.21063529867937253No Hit
CTTTAGGACGTGAAATATGGCGAGG28020.1886225972833499No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23470.15799330329194228No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19690.13254742828369592No Hit
GTACTGGTTCACTATCGGTCAGTCA19370.13039328013484966No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15860.10676496762719236No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATT250.00607303318.97767614
TTGCGAG601.5138667E-615.78531818
ATTGCGA653.4144305E-614.59771717
GGTATCA13700.013.9942611
TAGGACC4600.012.783244
TATTGCG751.4969617E-512.65135516
GTCCTAC17150.011.6400821
GCCCTAA853.5576835E-511.6265251
TCCTACA19150.011.1431022
AATGTCC4250.011.1185168
ATTTCTA13100.011.08238315
TTTCGAA600.00600974211.04972318
CCTACAG19200.010.9662683
GGCGAGG9100.010.93162419
ACTACAC1502.1900632E-910.7489183
TGTCCAC4900.010.63704310
AAATGTC4250.010.4892747
CATTTCT24350.010.40459814
GGTCTAG951.10280365E-410.4026791
GTACTAA1052.657355E-510.3531441
Produced by FastQC (version 0.11.5)