FastQCFastQC Report
Thu 26 May 2016
SRR1512353_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512353_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1485506
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT93760.6311654076119517No Hit
GGTATCAACGCAGAGTACTTTTTTT66880.4502169631088666No Hit
TATCAACGCAGAGTACTTTTTTTTT62450.42039547467327637No Hit
CTGTAGGACGTGGAATATGGCAAGA37890.2550646042493265No Hit
GTCCTAAAGTGTGTATTTCTCATTT34140.22982068063003447No Hit
CTTTAGGACGTGAAATATGGCGAGG34090.22948409498177724No Hit
GTCCTACAGTGGACATTTCTAAATT33050.2224831134980269No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30990.2086157847898292No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23730.15974354866287985No Hit
GTACTGGTTCACTATCGGTCAGTCA19660.13234547689474158No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19580.13180693985753003No Hit
CTGAAGGACCTGGAATATGGCGAGA16900.11376594911094266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16050.013.6369351
TAGGACC4850.011.7619164
CCGTTCG1001.9404997E-611.39446616
GGCGAGG9200.011.35318319
TATTAGG1354.660251E-911.2693462
CTTATAC1701.0913936E-1111.1853523
TAATACT1451.200533E-911.1467824
GGTCTAA600.005801358711.1022431
GTTCGCT1201.2834062E-711.07795318
CGTTCGC1053.4854856E-610.85187217
TCCAACG700.001497387410.85187218
GTCCTAC15450.010.8404711
GTGGTAT4350.010.7194071
TTAGACA1803.45608E-1110.5639444
TATACAG3150.010.5639445
TGTAGGA17650.010.2896632
CCCGTTC1201.5319074E-610.28701715
TAGGACA4100.010.2032234
GTCTTAT1409.443829E-810.1959381
TAGAAAT5500.010.0261434
Produced by FastQC (version 0.11.5)