##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512353_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1485506 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.88049189972979 34.0 31.0 34.0 30.0 34.0 2 32.050217232377385 34.0 31.0 34.0 30.0 34.0 3 32.16891146855011 34.0 31.0 34.0 30.0 34.0 4 35.6175787913344 37.0 35.0 37.0 33.0 37.0 5 35.421523709766234 37.0 35.0 37.0 33.0 37.0 6 35.45133712014626 37.0 35.0 37.0 33.0 37.0 7 35.36153808870513 37.0 35.0 37.0 33.0 37.0 8 35.325405619364716 37.0 35.0 37.0 33.0 37.0 9 36.96264437841382 39.0 37.0 39.0 33.0 39.0 10 36.89526329748921 39.0 37.0 39.0 33.0 39.0 11 36.93339373923767 39.0 37.0 39.0 33.0 39.0 12 36.861822840163555 39.0 37.0 39.0 33.0 39.0 13 36.83654189212295 39.0 37.0 39.0 33.0 39.0 14 38.15275535743376 40.0 38.0 41.0 33.0 41.0 15 38.134694844719576 40.0 38.0 41.0 33.0 41.0 16 38.0900386804227 40.0 38.0 41.0 33.0 41.0 17 38.08999627063102 40.0 38.0 41.0 33.0 41.0 18 38.048656148140765 40.0 38.0 41.0 33.0 41.0 19 38.07600171254778 40.0 38.0 41.0 33.0 41.0 20 38.063410716617774 40.0 38.0 41.0 33.0 41.0 21 38.01595348655609 40.0 38.0 41.0 33.0 41.0 22 37.95593622644405 40.0 38.0 41.0 33.0 41.0 23 37.882983979869486 40.0 38.0 41.0 33.0 41.0 24 37.820805839895634 40.0 38.0 41.0 33.0 41.0 25 37.75635372728215 40.0 38.0 41.0 33.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5723130913020533 1101 2 0.5972170922450672 1101 3 0.5366474855667107 1101 4 0.4913679671660418 1101 5 0.43631844198564096 1101 6 0.5782275990892032 1101 7 0.6236379582995824 1101 8 0.6579717731515871 1101 9 0.6784046599364757 1101 10 0.7676218835014694 1101 11 0.7690260094510748 1101 12 0.8466842210976324 1101 13 0.9166698833170699 1101 14 1.085549133080562 1101 15 1.0311917812812936 1101 16 1.0463850676885542 1101 17 0.9566932869253932 1101 18 0.8859867594617796 1101 19 0.9327347864960203 1101 20 0.8842904106223841 1101 21 0.8484644359498645 1101 22 0.9161801244454963 1101 23 0.8318415796661682 1101 24 0.9243392857140904 1101 25 0.9663290824345481 1102 1 0.5375201579394151 1102 2 0.47209848938673815 1102 3 0.5009746236448578 1102 4 0.41977718888711024 1102 5 0.2900841699455725 1102 6 0.4383647423649464 1102 7 0.508113541808207 1102 8 0.4452200473055754 1102 9 0.6102311265131419 1102 10 0.550614408033077 1102 11 0.4807456288056642 1102 12 0.6654642860501383 1102 13 0.7459009530799676 1102 14 0.871675476519556 1102 15 0.8371317786999342 1102 16 0.913522831885146 1102 17 0.8718809061823123 1102 18 1.018448274578411 1102 19 0.9314279748547989 1102 20 0.9322482612595238 1102 21 0.8983260368791548 1102 22 0.961152845788078 1102 23 0.7920681983335598 1102 24 0.7448805159047964 1102 25 0.8552274188550442 1103 1 0.2694334791313828 1103 2 0.40781585443342294 1103 3 0.37672670345109793 1103 4 0.264117564465117 1103 5 0.37398780820822 1103 6 0.3763495015548628 1103 7 0.4494805391574701 1103 8 0.5158750150228641 1103 9 0.7218160777528553 1103 10 0.7282353283889691 1103 11 0.6910527199425971 1103 12 0.6834211929927108 1103 13 0.6902970080152571 1103 14 0.7680993032624883 1103 15 0.8023189832568463 1103 16 0.7727759743752785 1103 17 0.8789960493335656 1103 18 0.8228867865947223 1103 19 0.7315126879175793 1103 20 0.825752378138418 1103 21 0.8521046291089291 1103 22 0.7503819694854883 1103 23 0.8613104437576098 1103 24 0.929482094282605 1103 25 0.8811088934656368 1104 1 0.21957240773426534 1104 2 0.17973687365338975 1104 3 0.1791979394592076 1104 4 0.04367694221618734 1104 5 0.02627780764116494 1104 6 0.09241153018996329 1104 7 0.1131369855532256 1104 8 0.12046076487737878 1104 9 0.17501972026014556 1104 10 0.13951989414307775 1104 11 0.1819740001689638 1104 12 0.18470269825566987 1104 13 0.06408030191611402 1104 14 0.10374710789296415 1104 15 0.1742997644984854 1104 16 0.1458035964138702 1104 17 0.28340798259475264 1104 18 0.33176200984770077 1104 19 0.2517901509996534 1104 20 0.27277994146061957 1104 21 0.2406900829685057 1104 22 0.26748512814561565 1104 23 0.21608852999750638 1104 24 0.3435858509651126 1104 25 0.30740434387059423 1105 1 0.39143462516420513 1105 2 0.38951239536714155 1105 3 0.43198680349750873 1105 4 0.30669622390236384 1105 5 0.2177865685788305 1105 6 0.30148274829386423 1105 7 0.37020704460929466 1105 8 0.40865520273728606 1105 9 0.4460139034025943 1105 10 0.45535853314177643 1105 11 0.3863301388557119 1105 12 0.45885088722446454 1105 13 0.49898266085912013 1105 14 0.6026053168227108 1105 15 0.5385244786904124 1105 16 0.5812278302988005 1105 17 0.6377106253072569 1105 18 0.48287059586623116 1105 19 0.44612522884662553 1105 20 0.470708219307447 1105 21 0.5119421694252821 1105 22 0.5632604063682933 1105 23 0.6336692679574938 1105 24 0.7032923266518409 1105 25 0.7067088393149987 1106 1 0.3306105981761185 1106 2 0.2911275282948367 1106 3 0.2284610846752244 1106 4 0.1349122809794494 1106 5 0.07776344617272457 1106 6 0.19918888013788205 1106 7 0.13329461773501805 1106 8 0.24643949966601753 1106 9 0.4068469672483701 1106 10 0.37956693054842106 1106 11 0.35674451238862304 1106 12 0.24477333007380508 1106 13 0.3451301588071516 1106 14 0.37185886490962616 1106 15 0.5043606755624666 1106 16 0.4978396658937143 1106 17 0.43741128873210755 1106 18 0.47329974204748737 1106 19 0.5395028552300687 1106 20 0.5512255009907818 1106 21 0.5153616118138231 1106 22 0.4772298946506268 1106 23 0.49567757141069535 1106 24 0.590102323358245 1106 25 0.6140250344842357 1107 1 0.36265298101877974 1107 2 0.34402406528500507 1107 3 0.35529995097739686 1107 4 0.32993032857453386 1107 5 0.31358362155165764 1107 6 0.32857505386368757 1107 7 0.2932548818196281 1107 8 0.3937404420137369 1107 9 0.48060485982056633 1107 10 0.4735120444151022 1107 11 0.34024122493376296 1107 12 0.4880813970407445 1107 13 0.4611128625617411 1107 14 0.5002136331794631 1107 15 0.4684959313054122 1107 16 0.4700569315730405 1107 17 0.48059349062672396 1107 18 0.5396489588114619 1107 19 0.5268707389124998 1107 20 0.6173414712920646 1107 21 0.6863496726355365 1107 22 0.6803459553699227 1107 23 0.7500630449970345 1107 24 0.6184311664637931 1107 25 0.59745053284351 1108 1 0.2916776976349347 1108 2 0.25671481409361974 1108 3 0.26109485835397095 1108 4 0.1547918309619405 1108 5 0.23373047470933983 1108 6 0.24537659146983515 1108 7 0.2385059276179291 1108 8 0.22973673428899843 1108 9 0.29461112299468795 1108 10 0.0833181555012672 1108 11 0.20397266114277812 1108 12 0.2026342166306705 1108 13 0.22511053013339932 1108 14 0.30750138762090984 1108 15 0.3000120561909938 1108 16 0.4649491303281508 1108 17 0.4365983320417257 1108 18 0.38072052843042314 1108 19 0.31773121292513196 1108 20 0.44009395356266623 1108 21 0.25760799139764856 1108 22 0.2176826195637389 1108 23 0.3138923626610719 1108 24 0.36788484236156904 1108 25 0.37458243584794815 1109 1 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1110 12 0.053347683213836206 1110 13 0.10554809037108726 1110 14 -0.03641655820105427 1110 15 0.09765365714501684 1110 16 0.20298243595149046 1110 17 0.03112397850744486 1110 18 -0.08262617089933144 1110 19 -0.0249125727541184 1110 20 0.029193673255321073 1110 21 0.07290564246311249 1110 22 0.16690865896450902 1110 23 0.037491101987860986 1110 24 0.044382246718711826 1110 25 0.10710147923801117 1111 1 -0.015110376108626866 1111 2 -0.13120846770223338 1111 3 -0.11678311196874347 1111 4 -0.18364094548992682 1111 5 -0.15101512385175653 1111 6 -0.08827980229032306 1111 7 -0.12976047617301845 1111 8 -0.05676355971674241 1111 9 -0.06866617630316085 1111 10 -0.19463418097966212 1111 11 -0.10906009455574406 1111 12 -0.17820456445611654 1111 13 -0.2718397656017899 1111 14 -0.2362345606340739 1111 15 -0.12439180393144511 1111 16 -0.18395128819767592 1111 17 -0.20891548622813616 1111 18 -0.25744383807367655 1111 19 -0.24546756954521243 1111 20 -0.25993302578775257 1111 21 -0.28934576322861716 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7 0.09210733575897478 1113 8 0.0690554175870588 1113 9 0.07645985113816778 1113 10 0.14868837527828305 1113 11 -0.018293635387991003 1113 12 0.08490438051645555 1113 13 0.1080733743875939 1113 14 0.10685122166899674 1113 15 -0.05869571745748203 1113 16 0.06734662555355442 1113 17 -0.09933032320476087 1113 18 0.0029653690874482663 1113 19 0.004561544643529203 1113 20 0.04538666730687879 1113 21 0.05902593327606809 1113 22 -0.04201027006565283 1113 23 -0.019623963669523903 1113 24 0.09983873077513294 1113 25 0.1776473147017228 1114 1 -0.3987711860594061 1114 2 -0.49180332313721564 1114 3 -0.4314129788029746 1114 4 -0.4874467635369868 1114 5 -0.5489537532030084 1114 6 -0.5421419318147755 1114 7 -0.5980463999148711 1114 8 -0.8335319737309703 1114 9 -0.9073192122087761 1114 10 -0.9073289953290171 1114 11 -0.8104993804714269 1114 12 -0.7233844147755377 1114 13 -0.7952571230709111 1114 14 -1.1383605404457242 1114 15 -1.0018096930969236 1114 16 -0.9951301234604202 1114 17 -1.1154078209390406 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2315 11 -1.0354486504185019 2315 12 -1.1592878101152237 2315 13 -1.154595753955391 2315 14 -1.3195976624258918 2315 15 -1.3457996481241494 2315 16 -1.5021322314907977 2315 17 -1.4912052923661463 2315 18 -1.341882192897387 2315 19 -1.3223099924530572 2315 20 -1.3172039513829148 2315 21 -1.3625040743375578 2315 22 -1.1449609713748359 2315 23 -1.1976691170256686 2315 24 -1.3086831579285345 2315 25 -1.3004521146199153 2316 1 -1.1506251970953443 2316 2 -1.0398875278734927 2316 3 -1.0009357295375096 2316 4 -0.8246298288481455 2316 5 -0.8454793103747278 2316 6 -0.8889431938399426 2316 7 -1.0002404178845907 2316 8 -0.9154764531455868 2316 9 -1.080096190514574 2316 10 -1.1422562584426714 2316 11 -1.2511003337691378 2316 12 -1.2188803639381547 2316 13 -1.1804691940433685 2316 14 -1.3424363626698437 2316 15 -1.475479734331607 2316 16 -1.5552012431107087 2316 17 -1.6853353648900935 2316 18 -1.8488338872004277 2316 19 -1.7764618633390796 2316 20 -1.663643286137912 2316 21 -1.6768391444623418 2316 22 -1.7568876614271716 2316 23 -1.8500082112479816 2316 24 -1.6941825132494017 2316 25 -1.6143762595692195 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10894.0 3 0.0 4 216.0 5 1867.0 6 2375.0 7 1827.0 8 1567.0 9 1453.0 10 1303.0 11 1212.0 12 1148.0 13 1121.0 14 1137.0 15 1126.0 16 1150.0 17 1270.0 18 1395.0 19 1578.0 20 1976.0 21 2514.0 22 3405.0 23 4221.0 24 5180.0 25 6154.0 26 7190.0 27 8127.0 28 9293.0 29 11566.0 30 15067.0 31 19412.0 32 25446.0 33 34004.0 34 46070.0 35 63485.0 36 95674.0 37 181184.0 38 912899.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.69688226341864 19.561020639487644 13.48434388761934 24.25775320947438 2 15.066424673598835 20.823162398801166 41.127152726259254 22.983260201340748 3 18.62920782548169 25.533791179571136 30.370055725119926 25.466945269827253 4 12.520086032507901 17.68452727560359 38.37440297276647 31.420983719122038 5 12.84086788795689 38.202004871322416 35.709933912302716 13.24719332841798 6 30.89269768926492 37.5643610494942 18.300625866172116 13.242315395068763 7 27.331738797849532 32.176015708329295 22.471435728368725 18.02080976545245 8 24.858078404148802 36.95146150122109 20.78973787850528 17.400722216124827 9 26.539100264929026 14.788750415845639 20.703523273213616 37.96862604601172 10 15.650488627425629 28.729622566290637 34.2122700972945 21.407618708989236 11 33.60596177455188 22.389720168062134 25.357408141949065 18.646909915436922 12 23.661677806023672 24.83274129194094 31.89858415124834 19.60699675078705 13 29.289130608977054 22.22218481351723 26.0747951143932 22.41388946311251 14 20.795234062263106 23.08698011409921 28.045933212780085 28.0718526108576 15 22.760736072408864 31.575535078429695 23.941204152845504 21.722524696315933 16 21.463096428999386 28.100272910992242 28.632361831236796 21.804268828771576 17 21.076058097766673 28.907920317523573 28.367427445109243 21.64859413960051 18 20.977409595973874 26.656380553861396 31.358978605154636 21.00723124501009 19 23.069849600068935 26.568522779443505 28.78170805099407 21.57991956949349 20 23.525384616420265 26.645735527153715 29.339699738674902 20.489180117751125 21 22.638616067521774 26.357820163634475 28.43542873606704 22.56813503277671 22 23.101044686291168 27.941756618657866 28.427611816709327 20.529586878341643 23 21.71041780967159 27.732962635098975 29.25569763410603 21.300921921123404 24 22.91979971807586 26.400566540963144 29.617315581357463 21.062318159603528 25 22.16052981273721 27.253945793554518 29.506646220210484 21.078878173497785 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 387.0 1 387.0 2 637.5 3 888.0 4 888.0 5 888.0 6 2024.0 7 3160.0 8 3160.0 9 3160.0 10 3869.0 11 4578.0 12 4578.0 13 4578.0 14 6035.0 15 7492.0 16 7492.0 17 7492.0 18 13463.5 19 19435.0 20 19435.0 21 19435.0 22 30621.5 23 41808.0 24 41808.0 25 41808.0 26 60717.0 27 79626.0 28 79626.0 29 79626.0 30 101004.0 31 122382.0 32 122382.0 33 122382.0 34 134746.0 35 147110.0 36 147110.0 37 147110.0 38 154171.0 39 161232.0 40 161232.0 41 161232.0 42 169613.5 43 177995.0 44 177995.0 45 177995.0 46 186254.0 47 194513.0 48 194513.0 49 194513.0 50 185842.5 51 177172.0 52 177172.0 53 177172.0 54 155545.0 55 133918.0 56 133918.0 57 133918.0 58 114252.5 59 94587.0 60 94587.0 61 94587.0 62 78397.5 63 62208.0 64 62208.0 65 62208.0 66 47415.5 67 32623.0 68 32623.0 69 32623.0 70 23921.5 71 15220.0 72 15220.0 73 15220.0 74 10687.5 75 6155.0 76 6155.0 77 6155.0 78 4125.0 79 2095.0 80 2095.0 81 2095.0 82 1385.0 83 675.0 84 675.0 85 675.0 86 430.0 87 185.0 88 185.0 89 185.0 90 118.0 91 51.0 92 51.0 93 51.0 94 30.5 95 10.0 96 10.0 97 10.0 98 5.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16660989588732728 2 0.004308296297692503 3 0.0 4 0.0014136597226803527 5 0.11578546300048603 6 0.03527417593735738 7 0.029215634268727288 8 0.023897581026263104 9 0.03904393519783832 10 0.03567807871526604 11 0.038976618068186863 12 0.03574539584491749 13 0.027532706027441158 14 0.008885861113990787 15 0.010972692133185594 16 0.0026926851860578148 17 6.731712965144537E-4 18 5.385370372115629E-4 19 0.0 20 0.0 21 0.0 22 0.005654638890721411 23 0.0014136597226803527 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1485506.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.82626173399821 #Duplication Level Percentage of deduplicated Percentage of total 1 82.2763921036044 54.15947321140927 2 12.136103492821974 15.97748649898778 3 2.953175295895938 5.831894699220709 4 1.034411174149467 2.723656827605409 5 0.48255515011134686 1.5882400806159154 6 0.26773394363962927 1.0574354789458658 7 0.16353640477615375 0.753549312868252 8 0.11393183686868698 0.59997655308427 9 0.08146741572827593 0.4826425887469741 >10 0.41729962575060364 4.9283377164598745 >50 0.035862936822785844 1.6645789749096969 >100 0.03078141657816104 4.073126938107415 >500 0.004294947524351162 1.9766863762698261 >1k 0.002147473762175581 2.681198537483516 >5k 3.0678196602508295E-4 1.5017162052853243 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 9376 0.6311654076119517 No Hit GGTATCAACGCAGAGTACTTTTTTT 6688 0.4502169631088666 No Hit TATCAACGCAGAGTACTTTTTTTTT 6245 0.42039547467327637 No Hit CTGTAGGACGTGGAATATGGCAAGA 3789 0.2550646042493265 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3414 0.22982068063003447 No Hit CTTTAGGACGTGAAATATGGCGAGG 3409 0.22948409498177724 No Hit GTCCTACAGTGGACATTTCTAAATT 3305 0.2224831134980269 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3099 0.2086157847898292 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2373 0.15974354866287985 No Hit GTACTGGTTCACTATCGGTCAGTCA 1966 0.13234547689474158 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1958 0.13180693985753003 No Hit CTGAAGGACCTGGAATATGGCGAGA 1690 0.11376594911094266 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 6.731712965144536E-5 9 0.0 0.0 0.0 0.0 1.3463425930289072E-4 10 0.0 0.0 0.0 0.0 1.3463425930289072E-4 11 0.0 0.0 0.0 0.0 1.3463425930289072E-4 12 0.0 0.0 0.0 0.0 1.3463425930289072E-4 13 0.0 0.0 0.0 0.0 1.3463425930289072E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1605 0.0 13.636935 1 TAGGACC 485 0.0 11.761916 4 CCGTTCG 100 1.9404997E-6 11.394466 16 GGCGAGG 920 0.0 11.353183 19 TATTAGG 135 4.660251E-9 11.269346 2 CTTATAC 170 1.0913936E-11 11.185352 3 TAATACT 145 1.200533E-9 11.146782 4 GGTCTAA 60 0.0058013587 11.102243 1 GTTCGCT 120 1.2834062E-7 11.077953 18 CGTTCGC 105 3.4854856E-6 10.851872 17 TCCAACG 70 0.0014973874 10.851872 18 GTCCTAC 1545 0.0 10.840471 1 GTGGTAT 435 0.0 10.719407 1 TTAGACA 180 3.45608E-11 10.563944 4 TATACAG 315 0.0 10.563944 5 TGTAGGA 1765 0.0 10.289663 2 CCCGTTC 120 1.5319074E-6 10.287017 15 TAGGACA 410 0.0 10.203223 4 GTCTTAT 140 9.443829E-8 10.195938 1 TAGAAAT 550 0.0 10.026143 4 >>END_MODULE