FastQCFastQC Report
Thu 26 May 2016
SRR1512352_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512352_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762457
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT77840.44165616522842827No Hit
GGTATCAACGCAGAGTACTTTTTTT50320.2855105117458185No Hit
TATCAACGCAGAGTACTTTTTTTTT45250.2567438524741313No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23460.13310963047609103No Hit
GTACTGGTTCACTATCGGTCAGTCA22110.12544986913155895No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19690.11171903768432365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14800.013.82428551
TAGGACC4000.013.0402344
GTATAAG1750.012.4256371
ACCCGTC701.1085668E-412.1934657
TGCACCG951.0612966E-611.9798865
GTATTAC1052.1544183E-611.2960341
AACCCGT600.00595125211.064446
GTCCTAG1706.730261E-1111.0468571
ACCGTGT600.006103293511.0269968
GTAGGAC10350.010.9040863
GTGGTAT5100.010.8530521
GTCCTAA5500.010.78257751
GTCCTAT1852.7284841E-1110.6854381
GTTCTAA1405.4207703E-810.5900331
CGTATCA854.5776163E-410.4654441
GGCTTAT854.5776163E-410.4654441
CGAACCG1553.805326E-910.39506812
GTCCTAC8600.010.2288211
AACACTA1303.930545E-710.2136225
TGTAGGA11750.010.1698682
Produced by FastQC (version 0.11.5)