FastQCFastQC Report
Thu 26 May 2016
SRR1512352_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512352_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762457
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT96380.546850221026669No Hit
GGTATCAACGCAGAGTACTTTTTTT66540.37754112582604854No Hit
TATCAACGCAGAGTACTTTTTTTTT64040.36335638259543357No Hit
ACGCAGAGTACTTTTTTTTTTTTTT31610.17935189340789592No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23370.13259897971978893No Hit
GTACTGGTTCACTATCGGTCAGTCA22090.12533639118571402No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20080.11393185762829958No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCGT350.002174711816.28082711
AACCGCT400.00528701214.2469357
GGTATCA15550.014.200811
TAGGACC4250.013.1988834
ACCGCTC550.003073674412.0883088
AGGACCG1354.669346E-911.2683515
GGACCGT855.3321546E-511.1747026
TTAGGAC6100.011.0663053
GTATTAC1053.3878023E-610.877961
CGGCGAC700.001494252610.8548079
TAGGACG10600.010.8530954
AGGACGT10300.010.7999755
TGGTATC4850.010.7822012
TAGGCCC803.7431892E-410.696134
GTAGGAC10950.010.5061933
CTTTAGG6650.010.4485681
GTATCAA33550.010.3551391
TGTAGGA11600.010.3275912
GGCGAGG5300.010.21188519
GGACGTG10250.010.1935076
Produced by FastQC (version 0.11.5)