FastQCFastQC Report
Thu 26 May 2016
SRR1512351_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512351_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69379
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26273.7864483489240257No Hit
GGTATCAACGCAGAGTACTTTTTTT21743.1335130226725667No Hit
TATCAACGCAGAGTACTTTTTTTTT17122.4676054713962436No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8991.2957811441502471No Hit
GTACTGGTTCACTATCGGTCAGTCA8601.2395681690425056No Hit
GTACTTTTTTTTTTTTTTTTTTTTT7781.1213767854826389No Hit
GAGTACTTTTTTTTTTTTTTTTTTT5150.7422995430894075No Hit
GGATACCACGTGTCCCGCCCTACTC4530.6529353262514594No Hit
ATACAGGGTGACAGCCCCGTACACA4460.6428458178987878No Hit
GTACATGGGGGTTAAGCGACTAAGC4150.5981637094798138No Hit
ATCATTAACTGAATCCATAGGTTAA3960.570777901093991No Hit
GTGGTATCAACGCAGAGTACATGGG3920.5650124677496072No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA3870.5578056760691276No Hit
GCAGAGTACTTTTTTTTTTTTTTTT3810.5491575260525519No Hit
GTATTTAGCCTTGGAGGATGGTCCC3680.5304198676833047No Hit
GGTATCAACGCAGAGTACATGGGGG3660.5275371510111129No Hit
GTGGTATCAACGCAGAGTACTTTTT3250.46844145923117947No Hit
GTGGATGCCCTGGCAGTCAGAGGCG3210.46267602588679574No Hit
GTTCACTATCGGTCAGTCAGGAGTA3160.4554692342063161No Hit
CCCATATTCAGACAGGATACCACGT3120.44970380086193235No Hit
GTATCAACGCAGAGTACATGGGGGT3090.4453797258536445No Hit
GGTTAATGAGGCGAACCGGGGGAAC2920.4208766341400136No Hit
CTATCGGTCAGTCAGGAGTATTTAG2730.3934908257541908No Hit
GTCAGGAGTATTTAGCCTTGGAGGA2690.387725392409807No Hit
TATTCAGACAGGATACCACGTGTCC2630.3790772423932314No Hit
ACTTAGATGTTTCAGTTCCCCCGGT2580.3718704507127517No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG2550.36754637570446386No Hit
GCCTTGGAGGATGGTCCCCCCATAT2480.35745686735179233No Hit
GCCCAGAGCCTGAATCAGTGTGTGT2360.3401605673186411No Hit
TCACGGTACTGGTTCACTATCGGTC2340.3372778506464492No Hit
TCTAAGTACCCCGAGGAAAAGAAAT2320.33439513397425735No Hit
AGCGTACACGGTGGATGCCCTGGCA2320.33439513397425735No Hit
GCCTGAATCAGTGTGTGTGTTAGTG2200.3170988339411061No Hit
GGTTCACTATCGGTCAGTCAGGAGT2170.31277475893281825No Hit
CGGTACTGGTTCACTATCGGTCAGT2130.3070093255884345No Hit
ACGCAGAGTACATGGGGGTTAAGCG2120.3055679672523386No Hit
ACCCTGTATCGCGCGCCTTTCCAGA2030.2925957422274752No Hit
ATCAACGCAGAGTACTTTTTTTTTT2000.2882716672191874No Hit
GGTATCAACGCAGAGTACATGGGGA1950.2810648755387077No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT1890.2724167255221321No Hit
GTACTTAGATGTTTCAGTTCCCCCG1870.26953400884994017No Hit
GTTAATGATAGTGTGTCGAAACACA1850.2666512921777483No Hit
GTTATAACCGGCGATTTCCGAATGG1830.26376857550555644No Hit
CATCTAAGTACCCCGAGGAAAAGAA1820.2623272171694605No Hit
GGAGTATTTAGCCTTGGAGGATGGT1800.25944450049726864No Hit
GAGTACATGGGGGTTAAGCGACTAA1790.2580031421611727No Hit
GTGCTAATCTGCGATAAGCGTCGGT1630.23494140878363767No Hit
CCCCAGTAGCGGCGAGCGAACGGGG1610.2320586921114458No Hit
TATCAACGCAGAGTACATGGGGGTT1590.22917597543925397No Hit
GATATGAACCGTTATAACCGGCGAT1570.2262932587670621No Hit
GAATCAGTGTGTGTGTTAGTGGAAG1570.2262932587670621No Hit
GTCCCGCCCTACTCATCGAGCTCAC1540.22196918375877428No Hit
GGGTGACAGCCCCGTACACAAAAAT1520.21908646708658241No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG1520.21908646708658241No Hit
CACTATCATTAACTGAATCCATAGG1490.2147623920782946No Hit
GGTGATATGAACCGTTATAACCGGC1410.2032315253895271No Hit
TCATTAACCTATGGATTCAGTTAAT1340.19314201703685552No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA1300.1873765836924718No Hit
ATCCATAGGTTAATGAGGCGAACCG1210.17440435866760837No Hit
NTATCAACGCAGAGTACTTTTTTTT1200.17296300033151243No Hit
GCGTACACGGTGGATGCCCTGGCAG1130.16287349197884085No Hit
TCGGTAAGGTGATATGAACCGTTAT1120.16143213364274492No Hit
TCCCCCAGTAGCGGCGAGCGAACGG1100.15854941697055305No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT1100.15854941697055305No Hit
CCCTGGCAGTCAGAGGCGATGAAGG1090.15710805863445712No Hit
AGTCAGGAGTATTTAGCCTTGGAGG1070.15422534196226523No Hit
GAATATGGGGGGACCATCCTCCAAG1060.1527839836261693No Hit
NGTATCAACGCAGAGTACTTTTTTT1050.15134262529007336No Hit
CTATCATTAACTGAATCCATAGGTT1050.15134262529007336No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT1040.14990126695397743No Hit
CCTATGGATTCAGTTAATGATAGTG1040.14990126695397743No Hit
AGCTCACAGCATGTGCATTTTTGTG1030.14845990861788147No Hit
GGGTACTTAGATGTTTCAGTTCCCC1020.14701855028178556No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA1010.14557719194568963No Hit
GCCCTACTCATCGAGCTCACAGCAT1010.14557719194568963No Hit
TAAGTACCCCGAGGAAAAGAAATCA1000.1441358336095937No Hit
GGGAAACCCAGTGTGTTTCGACACA1000.1441358336095937No Hit
GTGGTATCCTGTCTGAATATGGGGG990.14269447527349774No Hit
GGTATCAACGCAGAGTACATGGGCA970.13981175860130587No Hit
ATTTAGCCTTGGAGGATGGTCCCCC960.13837040026520994No Hit
GGGGTTAAGCGACTAAGCGTACACG960.13837040026520994No Hit
AAGTACCCCGAGGAAAAGAAATCAA950.136929041929114No Hit
GTCCCCCCATATTCAGACAGGATAC950.136929041929114No Hit
GGGTTTCCCCATTCGGAAATCGCCG930.13404632525692212No Hit
CCGTTATAACCGGCGATTTCCGAAT920.13260496692082618No Hit
GGAGGATGGTCCCCCCATATTCAGA920.13260496692082618No Hit
CAGTAGCGGCGAGCGAACGGGGAGC910.13116360858473025No Hit
GTACGGGGCTGTCACCCTGTATCGC910.13116360858473025No Hit
CACACACACTGATTCAGGCTCTGGG910.13116360858473025No Hit
GTATCAACGCAGAGTACATGGGGAA900.12972225024863432No Hit
GATTCAGGCTCTGGGCTGCTCCCCG890.1282808919125384No Hit
GAGCAGCCCAGAGCCTGAATCAGTG880.12683953357644243No Hit
TTTGTGTACGGGGCTGTCACCCTGT870.1253981752403465No Hit
AACGCAGAGTACTTTTTTTTTTTTT860.12395681690425056No Hit
GTACATGGGCATCTAAGTACCCCGA860.12395681690425056No Hit
GATCAACGCAGAGTACTTTTTTTTT840.12107410023205868No Hit
CAACGCAGAGTACTTTTTTTTTTTT830.11963274189596275No Hit
CATTAACTGAATCCATAGGTTAATG820.1181913835598668No Hit
NATCAACGCAGAGTACTTTTTTTTT820.1181913835598668No Hit
GGGGAAACCCAGTGTGTTTCGACAC820.1181913835598668No Hit
TTCCAGACGCTTCCACTAACACACA810.11675002522377087No Hit
GTCAGAGGCGATGAAGGACGTGCTA800.11530866688767494No Hit
GCCCCGTACACAAAAATGCACATGC800.11530866688767494No Hit
GAGTAGGGCGGGACACGTGGTATCC790.11386730855157902No Hit
TAATGATAGTGTGTCGAAACACACT790.11386730855157902No Hit
CCCTCACGGTACTGGTTCACTATCG780.11242595021548309No Hit
CGGTGGATGCCCTGGCAGTCAGAGG770.11098459187938714No Hit
CCACGTGTCCCGCCCTACTCATCGA770.11098459187938714No Hit
GTATCGCGCGCCTTTCCAGACGCTT750.10810187520719526No Hit
GGGCTGTCACCCTGTATCGCGCGCC750.10810187520719526No Hit
ATGATAGTGTGTCGAAACACACTGG740.10666051687109933No Hit
GTACATGGGGAGCGAACGGGGAGCA740.10666051687109933No Hit
CGTGGTATCCTGTCTGAATATGGGG730.1052191585350034No Hit
GTCTGAATATGGGGGGACCATCCTC730.1052191585350034No Hit
CGTCTGGAAAGGCGCGCGATACAGG720.10377780019890745No Hit
AAAATGCACATGCTGTGAGCTCGAT720.10377780019890745No Hit
TCAACGCAGAGTACTTTTTTTTTTT710.10233644186281152No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC710.10233644186281152No Hit
GGTATCAACGCAGAGTACATGGGCG710.10233644186281152No Hit
GTATCAACGCAGAGTACATGGGGAG710.10233644186281152No Hit
GTACACAAAAATGCACATGCTGTGA700.10089508352671558No Hit
GTATCAACGCAGAGTACATGGGCAT700.10089508352671558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4400.015.9278191
GTGGTAT903.233872E-713.16101551
ACAGGGT957.1431714E-812.9791913
CGCCCTA751.4437257E-512.65555616
ATGGGAA604.059265E-412.6463915
ACTGAAT1052.0145308E-812.600778
TACAGGG1001.395365E-712.3302312
TGGTATC1103.664718E-812.0715562
TGAATCC1103.7396603E-812.05409810
GCCCTAC802.7996204E-511.86458317
CCGCCCT802.820528E-511.85599115
TTAACTG1156.7992005E-811.5467055
AATCCAT1156.9378075E-811.53000712
GAATCCA1156.9378075E-811.53000711
AGGGTGA1104.8089896E-711.2093025
CCCGCCC855.2388845E-511.15858114
ATACAGG1155.0898234E-711.1582521
ACCGGGG600.005849234311.06559215
TAACTGA1201.226199E-711.0655926
CAGGGTG1158.536281E-710.7219414
Produced by FastQC (version 0.11.5)