FastQCFastQC Report
Thu 26 May 2016
SRR1512351_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512351_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69379
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32584.695945459000562No Hit
GGTATCAACGCAGAGTACTTTTTTT29354.230386716441575No Hit
TATCAACGCAGAGTACTTTTTTTTT25533.6797878320529267No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11681.6835065365600543No Hit
GTACTTTTTTTTTTTTTTTTTTTTT8501.2251545856815462No Hit
GTACTGGTTCACTATCGGTCAGTCA7021.0118335519393478No Hit
GAGTACTTTTTTTTTTTTTTTTTTT6650.9585032935037979No Hit
ATCATTAACTGAATCCATAGGTTAA5350.7711267098113262No Hit
GCAGAGTACTTTTTTTTTTTTTTTT5160.7437409014255034No Hit
GGATACCACGTGTCCCGCCCTACTC5040.7264446013923521No Hit
GTGGTATCAACGCAGAGTACATGGG4980.7177964513757765No Hit
GTGGTATCAACGCAGAGTACTTTTT4690.6759970596289944No Hit
ATACAGGGTGACAGCCCCGTACACA4580.6601421179319391No Hit
GGTTAATGAGGCGAACCGGGGGAAC4150.5981637094798138No Hit
GTACATGGGGGTTAAGCGACTAAGC3870.5578056760691276No Hit
GGTATCAACGCAGAGTACATGGGGG3720.5361853010276885No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA3440.49582726761700224No Hit
TCTAAGTACCCCGAGGAAAAGAAAT3380.4871791176004266No Hit
GTGGATGCCCTGGCAGTCAGAGGCG3260.46988281756727535No Hit
ACTTAGATGTTTCAGTTCCCCCGGT3080.44393836751754856No Hit
TATTCAGACAGGATACCACGTGTCC3070.44249700918145257No Hit
ATCAACGCAGAGTACTTTTTTTTTT2880.4151112007956298No Hit
GTATCAACGCAGAGTACATGGGGGT2870.4136698424595338No Hit
GTATTTAGCCTTGGAGGATGGTCCC2870.4136698424595338No Hit
AGCGTACACGGTGGATGCCCTGGCA2820.4064630507790542No Hit
CGGTACTGGTTCACTATCGGTCAGT2800.40358033410686234No Hit
ACGCAGAGTACATGGGGGTTAAGCG2680.38628403407371104No Hit
CCCATATTCAGACAGGATACCACGT2620.37763588405713544No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT2600.3747531673849436No Hit
TCACGGTACTGGTTCACTATCGGTC2510.3617809423600801No Hit
CATCTAAGTACCCCGAGGAAAAGAA2460.35457415067960046No Hit
CTATCGGTCAGTCAGGAGTATTTAG2360.3401605673186411No Hit
GTTCACTATCGGTCAGTCAGGAGTA2310.3329537756381614No Hit
GTCAGGAGTATTTAGCCTTGGAGGA2300.3315124173020655No Hit
TATCAACGCAGAGTACATGGGGGTT2290.33007105896596955No Hit
GGTATCAACGCAGAGTACATGGGGA2250.32430562562158577No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG2200.3170988339411061No Hit
GTTAATGATAGTGTGTCGAAACACA2190.3156574756050102No Hit
GTTATAACCGGCGATTTCCGAATGG2120.3055679672523386No Hit
GGTTCACTATCGGTCAGTCAGGAGT2060.29691981723576294No Hit
GCCCAGAGCCTGAATCAGTGTGTGT2020.29115438389137926No Hit
ACCCTGTATCGCGCGCCTTTCCAGA1870.26953400884994017No Hit
GAGTACATGGGGGTTAAGCGACTAA1840.26520993384165237No Hit
GGAGTATTTAGCCTTGGAGGATGGT1820.2623272171694605No Hit
CACTATCATTAACTGAATCCATAGG1800.25944450049726864No Hit
GATATGAACCGTTATAACCGGCGAT1740.250796350480693No Hit
GCCTGAATCAGTGTGTGTGTTAGTG1730.24935499214459705No Hit
GAATCAGTGTGTGTGTTAGTGGAAG1680.24214820046411736No Hit
TCATTAACCTATGGATTCAGTTAAT1640.2363827671197336No Hit
GTGCTAATCTGCGATAAGCGTCGGT1630.23494140878363767No Hit
GGTGATATGAACCGTTATAACCGGC1600.23061733377534988No Hit
TCGGTAAGGTGATATGAACCGTTAT1580.22773461710315804No Hit
GTACTTAGATGTTTCAGTTCCCCCG1550.2234105420948702No Hit
CAACGCAGAGTACTTTTTTTTTTTT1550.2234105420948702No Hit
TAAGTACCCCGAGGAAAAGAAATCA1530.22052782542267835No Hit
GCCTTGGAGGATGGTCCCCCCATAT1520.21908646708658241No Hit
CAGTAGCGGCGAGCGAACGGGGAGC1430.20611424206171897No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG1420.20467288372562303No Hit
ATCCATAGGTTAATGAGGCGAACCG1410.2032315253895271No Hit
CCCCAGTAGCGGCGAGCGAACGGGG1410.2032315253895271No Hit
GGGTACTTAGATGTTTCAGTTCCCC1370.19746609204514334No Hit
TCAACGCAGAGTACTTTTTTTTTTT1340.19314201703685552No Hit
GGTATCAACGCAGAGTACATGGGCA1300.1873765836924718No Hit
GGGAAACCCAGTGTGTTTCGACACA1290.18593522535637586No Hit
GGGTGACAGCCCCGTACACAAAAAT1270.18305250868418396No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT1260.18161115034808803No Hit
GCGTACACGGTGGATGCCCTGGCAG1250.18016979201199212No Hit
AACGCAGAGTACTTTTTTTTTTTTT1240.17872843367589616No Hit
CTATCATTAACTGAATCCATAGGTT1230.17728707533980023No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA1220.1758457170037043No Hit
AAGTACCCCGAGGAAAAGAAATCAA1190.17152164199541647No Hit
TTTGTGTACGGGGCTGTCACCCTGT1180.17008028365932054No Hit
AGTCAGGAGTATTTAGCCTTGGAGG1160.16719756698712868No Hit
GGGTTTCCCCATTCGGAAATCGCCG1160.16719756698712868No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT1160.16719756698712868No Hit
CACACACACTGATTCAGGCTCTGGG1160.16719756698712868No Hit
TCCCCCAGTAGCGGCGAGCGAACGG1140.16431485031493678No Hit
CCTATGGATTCAGTTAATGATAGTG1110.15999077530664899No Hit
ATTTAGCCTTGGAGGATGGTCCCCC1080.15566670029836116No Hit
GGGGAAACCCAGTGTGTTTCGACAC1080.15566670029836116No Hit
CCGTTATAACCGGCGATTTCCGAAT1060.1527839836261693No Hit
GTATCAACGCAGAGTACATGGGGAA1050.15134262529007336No Hit
AGCTCACAGCATGTGCATTTTTGTG1020.14701855028178556No Hit
GGTATCAACGCAGAGTACATGGGAT970.13981175860130587No Hit
GTGGTATCCTGTCTGAATATGGGGG960.13837040026520994No Hit
CAGGGTGACAGCCCCGTACACAAAA960.13837040026520994No Hit
GGAGGATGGTCCCCCCATATTCAGA950.136929041929114No Hit
ATGATAGTGTGTCGAAACACACTGG940.13548768359301805No Hit
CATTAACCTATGGATTCAGTTAATG940.13548768359301805No Hit
GAATATGGGGGGACCATCCTCCAAG940.13548768359301805No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA920.13260496692082618No Hit
TTCCAGACGCTTCCACTAACACACA920.13260496692082618No Hit
AGGTTAATGAGGCGAACCGGGGGAA920.13260496692082618No Hit
GGGGTTAAGCGACTAAGCGTACACG900.12972225024863432No Hit
CATTAACTGAATCCATAGGTTAATG870.1253981752403465No Hit
CCCTGGCAGTCAGAGGCGATGAAGG860.12395681690425056No Hit
TATTTAGCCTTGGAGGATGGTCCCC840.12107410023205868No Hit
TAATGATAGTGTGTCGAAACACACT840.12107410023205868No Hit
CGGTGGATGCCCTGGCAGTCAGAGG830.11963274189596275No Hit
GTGTTTCGACACACTATCATTAACT820.1181913835598668No Hit
GAGTAGGGCGGGACACGTGGTATCC800.11530866688767494No Hit
CACGTGTCCCGCCCTACTCATCGAG800.11530866688767494No Hit
GTATCAACGCAGAGTACATGGGGAG790.11386730855157902No Hit
ATTCAGACAGGATACCACGTGTCCC780.11242595021548309No Hit
GAGCAGCCCAGAGCCTGAATCAGTG780.11242595021548309No Hit
GCCCTACTCATCGAGCTCACAGCAT770.11098459187938714No Hit
GTAGCGGCGAGCGAACGGGGAGCAG760.10954323354329121No Hit
CGTGGTATCCTGTCTGAATATGGGG760.10954323354329121No Hit
GATTCAGGCTCTGGGCTGCTCCCCG760.10954323354329121No Hit
GTCAGAGGCGATGAAGGACGTGCTA750.10810187520719526No Hit
CCACGTGTCCCGCCCTACTCATCGA750.10810187520719526No Hit
GGGCTGTCACCCTGTATCGCGCGCC750.10810187520719526No Hit
GTACATGGGGAGCGAACGGGGAGCA750.10810187520719526No Hit
ATGAAGGACGTGCTAATCTGCGATA740.10666051687109933No Hit
GTACGGGGCTGTCACCCTGTATCGC730.1052191585350034No Hit
ACCGAGATTCCCCCAGTAGCGGCGA700.10089508352671558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6550.015.6641231
CGCCCTA903.445166E-813.72222216
CCCCGGT500.001474512513.30000119
CCCCCGG500.001474512513.30000118
CTTTCCA801.922148E-613.062517
GCCCTAC956.996743E-813.017
CCTACTC956.996743E-813.019
CCCTACT956.996743E-813.018
ACAGGGT1102.557499E-912.9545463
CCGCCCT1051.8730134E-812.66666615
CCCGCCC1051.8730134E-812.66666614
TCCCGCC1051.8730134E-812.66666613
TTCCAGA853.7923583E-612.29411719
GTGGTAT1551.8189894E-1212.2580641
GTGTCCC1103.5848643E-812.09090910
GTCCCGC1103.5848643E-812.09090912
CATGGGT959.930991E-712.04
AGGGTGA1302.413799E-911.6923075
GCCTTTC907.177501E-611.61111115
TTTCCAG907.177501E-611.61111118
Produced by FastQC (version 0.11.5)