FastQCFastQC Report
Thu 26 May 2016
SRR1512350_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512350_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1769380
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT72010.40697871570832717No Hit
GGTATCAACGCAGAGTACTTTTTTT44340.2505962540550927No Hit
TATCAACGCAGAGTACTTTTTTTTT41770.2360713922390894No Hit
GTCCTACAGTGGACATTTCTAAATT32240.18221071787857895No Hit
CTGTAGGACGTGGAATATGGCAAGA30620.17305496840701262No Hit
GTCCTAAAGTGTGTATTTCTCATTT30460.17215069685426534No Hit
CTTTAGGACGTGAAATATGGCGAGG26360.14897873831511604No Hit
GTCCTACAGTGTGCATTTCTCATTT20250.11444686839457889No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19140.10817348449739456No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18250.10314347398523777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11850.014.590181
GGACCGA400.005338617614.2260886
TAGGACC7100.012.1552784
ACCGTGG701.1434705E-412.1530378
ACCGCCC550.003186442412.0302798
AGCGTGT907.891411E-611.5528878
TCCGTGC600.006100180611.0277578
GTCCTAA9600.011.0116791
GTCCTAG2800.010.9381691
TAACACC1159.0131834E-710.7208044
CTAACCT1252.2934182E-710.6218434
TGGTATC3400.010.5995282
TGTAGGA19400.010.5103842
GTATCAA25150.010.1742571
GTCCTAC18550.010.1725641
ATCCTAC2051.8189894E-1110.1206031
GTAGGAC19050.010.1060843
GGCGAGG9100.010.10105619
CGCGCCT856.7350327E-410.0356712
TCGCGCG856.8644603E-410.0149089
Produced by FastQC (version 0.11.5)