FastQCFastQC Report
Thu 26 May 2016
SRR1512350_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512350_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1769380
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84400.4770032440741955No Hit
GGTATCAACGCAGAGTACTTTTTTT58410.33011563372480757No Hit
TATCAACGCAGAGTACTTTTTTTTT54920.3103912104805073No Hit
CTGTAGGACGTGGAATATGGCAAGA34780.19656602877844218No Hit
GTCCTACAGTGGACATTTCTAAATT34150.1930054595394997No Hit
GTCCTAAAGTGTGTATTTCTCATTT30150.17039867072081746No Hit
CTTTAGGACGTGAAATATGGCGAGG29870.1688161955035097No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27140.15338706213475906No Hit
GTCCTACAGTGTGCATTTCTCATTT20420.11540765691937288No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19320.10919078999423526No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGC402.7640216E-416.6229238
GGTATCA14250.014.9551441
GTCGAGG655.4771186E-513.14774219
CGCCCTA1352.7284841E-1112.66078816
TAGGACC6950.012.3103664
TGGCCCG550.003053442612.0989765
TCGAACT951.0458771E-611.99443119
GTACTAA1052.6694397E-711.7790831
GGCGAGG8650.011.74599219
GTATTAG1156.933078E-811.5821081
CCGTACA752.0830845E-411.3947117
TGTCGAG1001.9406907E-611.39470918
TCCCGCC1553.1468517E-1011.02838513
TGTAGGA21350.010.8646942
GTAGGAC20500.010.7583913
TTCCGAA803.7874404E-410.6825416
CCCGTAC803.7874404E-410.6825416
TGGCGAG18500.010.41948718
CTGTAGG20950.010.3994141
GTCCCGC1659.331416E-1010.36116912
Produced by FastQC (version 0.11.5)