##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512350_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1769380 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.6042828561417 33.0 31.0 34.0 30.0 34.0 2 31.785592693485853 34.0 31.0 34.0 30.0 34.0 3 31.89990392114752 34.0 31.0 34.0 30.0 34.0 4 35.37877731182674 37.0 35.0 37.0 33.0 37.0 5 35.16145938125219 37.0 35.0 37.0 33.0 37.0 6 35.182307361900776 37.0 35.0 37.0 32.0 37.0 7 35.08098938611265 37.0 35.0 37.0 32.0 37.0 8 35.04188755383242 37.0 35.0 37.0 32.0 37.0 9 36.63401304411715 39.0 37.0 39.0 33.0 39.0 10 36.55298805231211 39.0 37.0 39.0 32.0 39.0 11 36.58666029908782 39.0 37.0 39.0 32.0 39.0 12 36.504400411443555 39.0 37.0 39.0 32.0 39.0 13 36.478657495846 39.0 37.0 39.0 32.0 39.0 14 37.75107834382665 40.0 38.0 41.0 32.0 41.0 15 37.729159931727494 40.0 38.0 41.0 32.0 41.0 16 37.67580677977597 40.0 38.0 41.0 32.0 41.0 17 37.66780058551583 40.0 38.0 41.0 32.0 41.0 18 37.61094677231573 40.0 38.0 41.0 32.0 41.0 19 37.64103753857283 40.0 38.0 41.0 32.0 41.0 20 37.61044716228283 40.0 38.0 41.0 32.0 41.0 21 37.564996213362875 40.0 38.0 41.0 32.0 41.0 22 37.497988560964856 40.0 38.0 41.0 32.0 41.0 23 37.419501181204716 40.0 38.0 41.0 32.0 41.0 24 37.355799206501715 40.0 38.0 41.0 32.0 41.0 25 37.28271428409951 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.49302354033189033 1101 2 0.5636647451446883 1101 3 0.5394977991614702 1101 4 0.38104058959082465 1101 5 0.4832812737125707 1101 6 0.5110648361953523 1101 7 0.6109958604480425 1101 8 0.5909204733128348 1101 9 0.7219473739060547 1101 10 0.7303993556619517 1101 11 0.7479175082008567 1101 12 0.6939672857524286 1101 13 0.8173117566804677 1101 14 0.884787417418984 1101 15 0.8424093488873439 1101 16 0.8724453518672064 1101 17 0.8670111481191114 1101 18 0.7961466422635155 1101 19 0.8341885419646005 1101 20 0.8465173406439206 1101 21 0.8031877421989719 1101 22 0.9370656717759402 1101 23 0.7339547074981212 1101 24 0.7513518294276196 1101 25 0.6518326379291821 1102 1 0.6284725058893912 1102 2 0.607439225147079 1102 3 0.6440713861732412 1102 4 0.542487940589389 1102 5 0.4934706498008694 1102 6 0.5329459539713 1102 7 0.6062826012743159 1102 8 0.63837170551745 1102 9 0.7911567098724248 1102 10 0.7742475871219341 1102 11 0.8018847813474963 1102 12 0.8372911415014173 1102 13 0.8338090180525057 1102 14 1.000046138386466 1102 15 1.0582212057867366 1102 16 0.9849297081579351 1102 17 1.0183198093593049 1102 18 1.0930896585842262 1102 19 0.9717068196545 1102 20 1.096560409786619 1102 21 1.1078322331217052 1102 22 1.0892405566994583 1102 23 0.99450807123376 1102 24 1.0138725631264833 1102 25 1.0908736411768132 1103 1 0.46575230372646814 1103 2 0.5267537854356839 1103 3 0.4974055531904362 1103 4 0.4868430617631745 1103 5 0.3280003736078996 1103 6 0.3979322063079138 1103 7 0.5129416781697387 1103 8 0.5966406854944211 1103 9 0.6745511349093576 1103 10 0.6558417422401845 1103 11 0.6717813774812598 1103 12 0.6276020568592244 1103 13 0.6868394859494487 1103 14 0.8590517917374711 1103 15 0.8468620466597656 1103 16 0.9693243914203222 1103 17 0.9825304108946895 1103 18 0.950918951528287 1103 19 0.929098807519523 1103 20 0.8881023622200814 1103 21 0.6968512633091848 1103 22 0.8348427390023048 1103 23 0.9035756283181016 1103 24 1.0110832409926616 1103 25 1.0284120434162816 1104 1 0.4267422767192244 1104 2 0.42506777899135884 1104 3 0.3915519461377279 1104 4 0.2960260991872019 1104 5 0.2293387708646435 1104 6 0.29264079298110346 1104 7 0.321649776842051 1104 8 0.40196203720459067 1104 9 0.4334235811992144 1104 10 0.4869542536107616 1104 11 0.528809138670141 1104 12 0.46450030449720003 1104 13 0.44347308454835144 1104 14 0.5868993473703554 1104 15 0.6436548777492916 1104 16 0.6988446823816972 1104 17 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0.6231269211758175 1107 17 0.6393004832223284 1107 18 0.698255397081553 1107 19 0.7678591986122569 1107 20 0.5630277871132066 1107 21 0.6627559993392609 1107 22 0.7826368509649129 1107 23 0.8225601699283018 1107 24 0.7555890904862395 1107 25 0.7857687769327271 1108 1 0.3069897760975593 1108 2 0.25742818315068305 1108 3 0.28125714935923085 1108 4 0.3258379245522036 1108 5 0.3403473422497214 1108 6 0.2277956513680195 1108 7 0.24967475514716142 1108 8 0.17937905107410046 1108 9 0.3073374410629057 1108 10 0.1552553533260621 1108 11 0.15537510382637265 1108 12 0.25610197107082655 1108 13 0.32418670691007634 1108 14 0.3798090509496319 1108 15 0.44760537787362153 1108 16 0.5240472949033332 1108 17 0.5349072540835991 1108 18 0.3755271004165692 1108 19 0.37480383673804596 1108 20 0.4287181210436728 1108 21 0.41441063392514366 1108 22 0.4094397858629506 1108 23 0.34232675113680244 1108 24 0.43203428105079666 1108 25 0.4840851795372316 1109 1 -0.051236818713821464 1109 2 -0.0030016952801936725 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-1.4759922898771762 2315 21 -1.3849504645093447 2315 22 -1.2836414260658557 2315 23 -1.2994042355891224 2315 24 -1.4112016958535065 2315 25 -1.5354429857041438 2316 1 -1.3260608088000012 2316 2 -1.3683132846035981 2316 3 -1.235267811138609 2316 4 -1.0370293791652045 2316 5 -1.0004664686798748 2316 6 -1.060901640354892 2316 7 -1.1869856855951681 2316 8 -1.1805380939707604 2316 9 -1.4224553772891113 2316 10 -1.3949015488192273 2316 11 -1.537663884918281 2316 12 -1.4947546836603607 2316 13 -1.4816020749184844 2316 14 -1.6817459400434345 2316 15 -1.7126240223574172 2316 16 -1.8864432400688074 2316 17 -2.0996583549136005 2316 18 -2.1785353152125637 2316 19 -2.0965656158084656 2316 20 -2.169347932767785 2316 21 -2.216030595267064 2316 22 -2.216144115844159 2316 23 -2.2968062815227412 2316 24 -2.248313559276376 2316 25 -1.969195380972998 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17727.0 3 0.0 4 386.0 5 2898.0 6 3741.0 7 2922.0 8 2299.0 9 2115.0 10 1906.0 11 1787.0 12 1717.0 13 1654.0 14 1599.0 15 1685.0 16 1689.0 17 1760.0 18 2045.0 19 2189.0 20 2831.0 21 3616.0 22 4664.0 23 5854.0 24 7081.0 25 8490.0 26 9518.0 27 10865.0 28 12347.0 29 15090.0 30 19280.0 31 25254.0 32 32982.0 33 43728.0 34 58795.0 35 81118.0 36 121494.0 37 241899.0 38 1014355.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.41050074748992 19.665296598680275 13.395098662443061 24.52910399138674 2 15.10968732302343 21.071699461143435 40.94100385349703 22.877609362336106 3 18.73271993579672 25.561722185172208 30.625755914501124 25.079801964529945 4 12.577393934632749 17.788496046869003 38.67190171344731 30.962208305050932 5 12.621779997951826 38.33958818147716 36.17022239648664 12.868409424084362 6 30.838724339836084 38.12485124440241 18.293334448941426 12.743089966820081 7 27.21865258963873 32.09367901798485 22.783552128004775 17.904116264371652 8 24.60116380689443 37.366183122651954 20.900588008515438 17.132065061938178 9 26.319535766743595 14.908472912753865 20.56727684724831 38.204714473254235 10 14.858785904200438 29.0712156391848 35.02512099744522 21.044877459169545 11 33.919224676298285 22.328379574820296 25.351122086797627 18.40127366208379 12 23.19598644509347 24.93998776575837 32.30258151544036 19.5614442737078 13 29.019784970793722 22.20982956739208 26.132679543847292 22.637705917966905 14 20.51494077723241 22.755266067241976 28.289243137777998 28.44055001774761 15 22.625060478492266 31.437115933313137 23.958469928058857 21.979353660135743 16 21.596418993576684 28.282207722110286 28.50285559263791 21.61851769167512 17 21.08053332067344 28.89434470067544 28.41236442185256 21.612757556798563 18 20.89608165618271 27.293217359851248 30.873305187722185 20.93739579624386 19 22.98387005617787 26.78797092766958 28.69134951225853 21.53680950389402 20 23.171110784568604 27.116447569205032 29.21452712249489 20.497914523731474 21 22.529869219726685 26.573658569668474 28.329414823271428 22.567057387333417 22 22.74246130611913 28.12789667162539 28.363925953725598 20.765716068529883 23 21.69200719353712 27.90739733893383 28.88797456263995 21.512620904889097 24 22.589438108263913 26.778419559393683 29.488521402977312 21.14362092936509 25 22.128259616362794 27.432773061750442 29.154393064237187 21.28457425764957 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 334.0 1 334.0 2 531.5 3 729.0 4 729.0 5 729.0 6 1830.0 7 2931.0 8 2931.0 9 2931.0 10 4075.0 11 5219.0 12 5219.0 13 5219.0 14 7570.5 15 9922.0 16 9922.0 17 9922.0 18 17362.0 19 24802.0 20 24802.0 21 24802.0 22 38704.0 23 52606.0 24 52606.0 25 52606.0 26 74249.5 27 95893.0 28 95893.0 29 95893.0 30 120486.0 31 145079.0 32 145079.0 33 145079.0 34 161407.0 35 177735.0 36 177735.0 37 177735.0 38 188314.5 39 198894.0 40 198894.0 41 198894.0 42 207180.0 43 215466.0 44 215466.0 45 215466.0 46 220508.0 47 225550.0 48 225550.0 49 225550.0 50 216350.5 51 207151.0 52 207151.0 53 207151.0 54 183109.0 55 159067.0 56 159067.0 57 159067.0 58 135779.0 59 112491.0 60 112491.0 61 112491.0 62 91887.5 63 71284.0 64 71284.0 65 71284.0 66 54283.5 67 37283.0 68 37283.0 69 37283.0 70 27141.5 71 17000.0 72 17000.0 73 17000.0 74 11796.5 75 6593.0 76 6593.0 77 6593.0 78 4468.5 79 2344.0 80 2344.0 81 2344.0 82 1539.5 83 735.0 84 735.0 85 735.0 86 470.0 87 205.0 88 205.0 89 205.0 90 131.5 91 58.0 92 58.0 93 58.0 94 31.5 95 5.0 96 5.0 97 5.0 98 4.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15830403870282245 2 0.004408323819643039 3 0.0 4 0.0012998903570742295 5 0.11026461246312266 6 0.030462647933174332 7 0.025206569532830708 8 0.020233075992720614 9 0.03470142083667725 10 0.030575681877267745 11 0.0351535566130509 12 0.03244074195480903 13 0.023228475511196015 14 0.00723417242197832 15 0.008816647639286078 16 0.0025432637421017533 17 7.347206366071731E-4 18 5.651697204670563E-4 19 0.0 20 0.0 21 0.0 22 0.006103832981044208 23 7.912376086538788E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1769380.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.64501281231605 #Duplication Level Percentage of deduplicated Percentage of total 1 83.14002483985887 57.90288095188245 2 11.623771279156756 16.190753993286073 3 2.804507080239438 5.859597946065201 4 0.979182566258052 2.7278072949055416 5 0.4501964018357436 1.5676967086954474 6 0.2450941130150458 1.0241749584693662 7 0.15567200504358178 0.7589245150045428 8 0.10579879859679373 0.5894686947041077 9 0.07770387323161067 0.4870518522103897 >10 0.3665900273750584 4.597417626766486 >50 0.027748787500767414 1.3473699081142472 >100 0.01940730016748149 2.709812119060546 >500 0.0028415560419954595 1.3928216565259792 >1k 0.0012178097322837682 1.726198690865165 >5k 2.4356194645675362E-4 1.1180230834444924 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8440 0.4770032440741955 No Hit GGTATCAACGCAGAGTACTTTTTTT 5841 0.33011563372480757 No Hit TATCAACGCAGAGTACTTTTTTTTT 5492 0.3103912104805073 No Hit CTGTAGGACGTGGAATATGGCAAGA 3478 0.19656602877844218 No Hit GTCCTACAGTGGACATTTCTAAATT 3415 0.1930054595394997 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3015 0.17039867072081746 No Hit CTTTAGGACGTGAAATATGGCGAGG 2987 0.1688161955035097 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2714 0.15338706213475906 No Hit GTCCTACAGTGTGCATTTCTCATTT 2042 0.11540765691937288 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1932 0.10919078999423526 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 5.6516972046705625E-5 0.0 11 0.0 0.0 0.0 1.6955091614011687E-4 0.0 12 0.0 0.0 0.0 1.6955091614011687E-4 0.0 13 0.0 0.0 0.0 1.6955091614011687E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTGCGC 40 2.7640216E-4 16.622923 8 GGTATCA 1425 0.0 14.955144 1 GTCGAGG 65 5.4771186E-5 13.147742 19 CGCCCTA 135 2.7284841E-11 12.660788 16 TAGGACC 695 0.0 12.310366 4 TGGCCCG 55 0.0030534426 12.098976 5 TCGAACT 95 1.0458771E-6 11.994431 19 GTACTAA 105 2.6694397E-7 11.779083 1 GGCGAGG 865 0.0 11.745992 19 GTATTAG 115 6.933078E-8 11.582108 1 CCGTACA 75 2.0830845E-4 11.39471 17 TGTCGAG 100 1.9406907E-6 11.394709 18 TCCCGCC 155 3.1468517E-10 11.028385 13 TGTAGGA 2135 0.0 10.864694 2 GTAGGAC 2050 0.0 10.758391 3 TTCCGAA 80 3.7874404E-4 10.68254 16 CCCGTAC 80 3.7874404E-4 10.68254 16 TGGCGAG 1850 0.0 10.419487 18 CTGTAGG 2095 0.0 10.399414 1 GTCCCGC 165 9.331416E-10 10.361169 12 >>END_MODULE