FastQCFastQC Report
Thu 26 May 2016
SRR1512349_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512349_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2157277
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69580.3225362343361562No Hit
GGTATCAACGCAGAGTACTTTTTTT44490.2062322084739234No Hit
TATCAACGCAGAGTACTTTTTTTTT39970.18527986902006557No Hit
GTCCTACAGTGGACATTTCTAAATT38300.17753862855813138No Hit
CTGTAGGACGTGGAATATGGCAAGA34100.15806964057003345No Hit
GTCCTAAAGTGTGTATTTCTCATTT32160.14907682230886438No Hit
CTTTAGGACGTGAAATATGGCGAGG27610.12798541865509158No Hit
GTCCTACAGTGTGCATTTCTCATTT22120.10253667007064923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13400.013.4187211
ATAGACG655.7081408E-513.0902338
CAGCGTA550.003167085112.0402719
CTAGTAC1451.0004442E-1011.7719083
ATTGCGA658.174744E-411.6666710
GGCGAGG9600.011.55058519
GCCTACG600.004388055311.5263371
TAGGACC7100.011.2192364
GTATTAT2650.011.1845861
CCCCGTA1605.5661076E-1010.67426315
GTCCTAA12450.010.6335911
CGAAATC1451.4812031E-810.46562613
AGGACGT20900.010.4357565
CGCGCCT1002.4545774E-510.42854412
GTCCTAG3700.010.4137561
CCCGTAC1553.756213E-910.40378116
TGGCGAG19700.010.33926418
GTGTAGA3250.010.3357051
AGGACCT12050.010.3092395
GGACGTG20300.010.2773026
Produced by FastQC (version 0.11.5)