FastQCFastQC Report
Thu 26 May 2016
SRR1512349_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512349_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2157277
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT89820.4163582145454664No Hit
GGTATCAACGCAGAGTACTTTTTTT61290.2841081604263152No Hit
TATCAACGCAGAGTACTTTTTTTTT59420.27543982529828115No Hit
CTGTAGGACGTGGAATATGGCAAGA39410.18268400395498585No Hit
GTCCTACAGTGGACATTTCTAAATT37950.17591621289245657No Hit
CTTTAGGACGTGAAATATGGCGAGG34840.16149989083460306No Hit
GTCCTAAAGTGTGTATTTCTCATTT33310.15440761663893882No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28470.13197192571932115No Hit
GTCCTACAGTGTGCATTTCTCATTT22820.1057815014019989No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6650.013.8670444
TACACCG1101.8371793E-1013.8280545
GGTATCA14650.013.6376061
CCCGTAC1251.03682396E-1012.91334816
CCGTACA1405.2750693E-1112.20799717
GGGCGAA550.003072420612.08916811
CCCCGTA1353.7471182E-1011.95763615
CTTAGAC1603.6379788E-1211.8834853
CGTGTGA658.035338E-411.69062410
CTAGTAC1406.730261E-1011.5439563
TCGAACT1657.2759576E-1211.50922319
TATGTCG1001.942526E-611.39413116
ACGGTAC1303.2337994E-810.969373
GGCGAGG7850.010.88611219
TGTAGGA22750.010.8236162
GTAGGAC21900.010.7656773
GCGCCAC1158.8607885E-710.73534313
CTGTAGG22200.010.7137991
GCGACTA1605.493348E-1010.68199816
TCCAACG803.7899415E-410.68199818
Produced by FastQC (version 0.11.5)