FastQCFastQC Report
Thu 26 May 2016
SRR1512348_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512348_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1945231
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75630.38879701176878223No Hit
GTCCTACAGTGGACATTTCTAAATT42870.2203851367780999No Hit
GGTATCAACGCAGAGTACTTTTTTT42230.21709503909818426No Hit
TATCAACGCAGAGTACTTTTTTTTT39680.2039860561547703No Hit
CTGTAGGACGTGGAATATGGCAAGA37920.19493828753500225No Hit
GTCCTAAAGTGTGTATTTCTCATTT37050.19046581100136692No Hit
CTTTAGGACGTGAAATATGGCGAGG30770.15818172751719461No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21250.10924152452844932No Hit
GTACTGGTTCACTATCGGTCAGTCA20520.10548875686229554No Hit
GTCCTACAGTGTGCATTTCTCATTT20160.10363807691734298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12850.014.0711121
TAGGACC6650.013.6888534
GCGCGAT550.003098050112.07566314
CGCGCGA550.003104652512.0722313
TAAGACG550.003111868612.0684874
GGACCGT802.9206423E-511.8535916
TTACACC1451.0004442E-1011.77123454
GGCGAGG10700.011.60417719
CGCGATA600.005931986511.06935715
TGTAGGA23100.011.0012052
TCCTATA2000.010.904742
CTTATAC1408.598363E-910.8370093
GCGCAGA1103.781046E-610.7787651
GTGTAGG3300.010.7787651
GCGAACC1855.456968E-1210.75878311
CTAGGAC3100.010.7059163
GTCTTAT1852.7284841E-1110.6816591
GTCCTAC19950.010.6481951
GACGTGA13250.010.5206217
TTAGGAC15100.010.4243423
Produced by FastQC (version 0.11.5)