FastQCFastQC Report
Thu 26 May 2016
SRR1512348_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512348_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1945231
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT95320.4900189232024371No Hit
TATCAACGCAGAGTACTTTTTTTTT60230.3096290363458119No Hit
GGTATCAACGCAGAGTACTTTTTTT59380.3052593753646739No Hit
CTGTAGGACGTGGAATATGGCAAGA43150.22182455451306296No Hit
GTCCTACAGTGGACATTTCTAAATT42140.2166323691119461No Hit
GTCCTAAAGTGTGTATTTCTCATTT37800.19432139422001807No Hit
CTTTAGGACGTGAAATATGGCGAGG35020.18003003242288448No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30160.155045853166025No Hit
GTCCTACAGTGTGCATTTCTCATTT20140.1035352613648456No Hit
CTGAAGGACCTGGAATATGGCGAGA19540.10045079478992469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC801.2918099E-714.24743610
GGTATCA16050.013.8700881
TAGGACC6900.012.4008724
TTAGACG550.003051231112.1002464
CTAATAC1451.202352E-911.1465313
CTACACT2400.011.0918914
GGCGAGG10250.011.02388119
GCGTGCG951.3632882E-510.998029
AGGACCG1658.0035534E-1110.9478415
GTGGTAT5050.010.9263471
GCTCGTG700.001493935410.85518911
TGTAGGA23950.010.7186332
ATTTAGA5500.010.5512891
GTATCAA34350.010.5243491
AGGACCT14700.010.4774715
CTGTAGG24050.010.4430471
GCACCGT1106.0595794E-610.3617726
GTAGGAC23500.010.3569273
TGGTATC4800.010.3001432
AATGTCC5850.010.2289288
Produced by FastQC (version 0.11.5)