FastQCFastQC Report
Thu 26 May 2016
SRR1512347_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512347_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1969240
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64280.32642034490463323No Hit
GTCCTACAGTGGACATTTCTAAATT62170.31570555138022793No Hit
GTCCTAAAGTGTGTATTTCTCATTT61800.31382665393755965No Hit
CTGTAGGACGTGGAATATGGCAAGA56700.2879283378359164No Hit
CTTTAGGACGTGAAATATGGCGAGG49120.24943633076719954No Hit
TATCAACGCAGAGTACTTTTTTTTT35800.181796022831143No Hit
GGTATCAACGCAGAGTACTTTTTTT34950.17747963681420245No Hit
GTCCTACAGTGTGCATTTCTCATTT27760.14096808921208182No Hit
CTGAAGGACCTGGAATATGGCGAGA25970.13187828807052468No Hit
GTCCTTCAGTGTGCATTTCTCATTT23300.11831975787613495No Hit
ATTTAGAAATGTCCACTGTAGGACG23150.11755804269667484No Hit
TTTCTAAATTTTCCACCTTTTTCAG22660.11506977311043853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11900.013.8109051
GCGCCAC1102.744855E-912.95552113
TAGGACC9300.012.3608294
TCTAGGA3400.012.0156092
TCCAACG1459.640644E-1111.7939918
TAGAAAT7450.011.3495584
CCCCGTA855.2733623E-511.187016515
GGCGAGG13850.011.1867719
GTGTAGG2950.010.9491381
CACCTTT9000.010.88248214
AATGTCC7450.010.6922988
CGACTAA1252.207853E-710.65140117
AGGACCT18800.010.5611565
GTCTAGG1909.094947E-1210.4999871
GGACCTG18800.010.45955856
TGTAGGA31200.010.4446962
ACCTTTT9700.010.3912415
GCGACTA1303.7860264E-710.24173216
GACGTGG16800.010.2348447
CCACCTT9700.010.088387513
Produced by FastQC (version 0.11.5)