FastQCFastQC Report
Thu 26 May 2016
SRR1512347_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512347_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1969240
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66730.33886169283581485No Hit
CTGTAGGACGTGGAATATGGCAAGA61430.3119477564948914No Hit
GTCCTAAAGTGTGTATTTCTCATTT60920.3093579248847271No Hit
GTCCTACAGTGGACATTTCTAAATT60590.3076821514899149No Hit
CTTTAGGACGTGAAATATGGCGAGG54800.27827994556275515No Hit
TATCAACGCAGAGTACTTTTTTTTT40300.2046474782149459No Hit
GGTATCAACGCAGAGTACTTTTTTT40090.20358107696370173No Hit
CTGAAGGACCTGGAATATGGCGAGA27940.14188214742743394No Hit
GTCCTACAGTGTGCATTTCTCATTT26650.1353313968840771No Hit
ATTTAGAAATGTCCACTGTAGGACG25590.1299486096158924No Hit
TTTCTAAATTTTCCACCTTTTTCAG23730.12050334139058723No Hit
GTCCTTCAGTGTGCATTTCTCATTT22560.11456196299079847No Hit
GAATATGGCAAGAAAACTGAAAATC21830.11085494911742601No Hit
CTGTAGGACCTGGAATATGGCGAGA20950.10638622006459345No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20040.10176514797586886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12700.014.1557241
TAGGACC8400.013.0098684
TCCAACG2050.012.50420118
AGGACCG951.0361364E-612.0039185
ACCGAGC657.994613E-411.6976128
GGACCGT855.2997606E-511.181546
GGCGAGG14550.011.09256619
CCGTCCA700.001486705810.8615159
AGGACGT32450.010.8355865
GGACGTG31850.010.8023856
CGTGCGC803.7753198E-410.68637110
GTGTAGG3050.010.6035941
TAGAAAT9500.010.6031914
CACCTTT7500.010.50795114
GACGTGG16500.010.425947
GACGTGA17150.010.3632997
TGTAGGA31700.010.3419672
CGTTTCC1752.382876E-1010.30900614
GCGCCAC1201.5270616E-610.29005713
TTAGGAC19500.010.282323
Produced by FastQC (version 0.11.5)