FastQCFastQC Report
Thu 26 May 2016
SRR1512346_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512346_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2237094
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT75700.3383854232321038No Hit
CTGTAGGACGTGGAATATGGCAAGA67170.30025559945178876No Hit
GTCCTAAAGTGTGTATTTCTCATTT63540.284029191442112No Hit
GTATCAACGCAGAGTACTTTTTTTT63540.284029191442112No Hit
CTTTAGGACGTGAAATATGGCGAGG55300.2471956922686306No Hit
GTCCTACAGTGTGCATTTCTCATTT42260.18890578580962625No Hit
GGTATCAACGCAGAGTACTTTTTTT34430.1539050214251167No Hit
TATCAACGCAGAGTACTTTTTTTTT34350.15354741463702465No Hit
CTGAAGGACCTGGAATATGGCGAGA28510.12744211910630487No Hit
CTGTAGGACCTGGAATATGGCGAGA28350.12672690553012078No Hit
ATTTAGAAATGTCCACTGTAGGACG24700.11041109582342092No Hit
GTCCTTCAGTGTGCATTTCTCATTT23930.10696913048803493No Hit
GAATATGGCAAGAAAACTGAAAATC23140.1034377634556259No Hit
TTTCTAAATTTTCCACCTTTTTCAG22880.10227554139432675No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCGA500.001508826313.289535510
GGTATCA13350.012.952751
GTCGAGG1155.285983E-912.39797419
CCAACGA701.08584034E-412.220860519
GTACCGT802.86836E-511.874726
TAGGACC11650.011.7430044
TGTACCG951.3601562E-511.0004825
TGTAGGA36800.010.8175352
ACCTTTT9450.010.76676115
TTAGGAC19600.010.7611583
AAATGTC8150.010.7243967
TAGAAAT8450.010.6809424
GTCCTAA20250.010.6036471
CCACCTT9350.010.5689713
AGGACCT23750.010.520465
AGGACGT36650.010.4984135
GGCGAGG15700.010.47378819
GGACGTG36500.010.4627666
GACGTGA17550.010.3936187
TTTAGGA22350.010.3297752
Produced by FastQC (version 0.11.5)