FastQCFastQC Report
Thu 26 May 2016
SRR1512346_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512346_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2237094
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT75540.3376702096559197No Hit
GTATCAACGCAGAGTACTTTTTTTT75520.33758080795889667No Hit
CTGTAGGACGTGGAATATGGCAAGA71580.31996867364536313No Hit
GTCCTAAAGTGTGTATTTCTCATTT65320.2919859424771601No Hit
CTTTAGGACGTGAAATATGGCGAGG60430.27012722755503343No Hit
TATCAACGCAGAGTACTTTTTTTTT45560.20365706581842338No Hit
GTCCTACAGTGTGCATTTCTCATTT42580.19033621296199443No Hit
GGTATCAACGCAGAGTACTTTTTTT41510.18555322217126327No Hit
CTGAAGGACCTGGAATATGGCGAGA31700.14170168978147543No Hit
CTGTAGGACCTGGAATATGGCGAGA30410.1359352803234911No Hit
ATTTAGAAATGTCCACTGTAGGACG26660.11917246213167618No Hit
GTCCTTCAGTGTGCATTTCTCATTT24070.10759494236719602No Hit
TTTCTAAATTTTCCACCTTTTTCAG23920.10692442963952341No Hit
GAATATGGCAAGAAAACTGAAAATC23000.10281195157646483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11650.013.5513511
TAGGACC12150.013.3748734
TAGTACG701.0878877E-412.2183994
TGTTCGG550.003071713712.08957110
TAGAAAT9800.011.9274854
GAAATGT9950.011.5587256
AAATGTC9550.011.445957
AATGTCC9700.011.36694058
GGACCTG23650.011.253166
ATTTAGA10000.011.2223151
AGGACCT24100.011.19879155
ATGTCCA10250.011.1261969
GACGAGC600.005853527711.08938
CGAATGG600.005905409311.07616119
CACCTTT8750.011.06786214
CCACCTT9050.011.02063413
TGTCCAC10400.010.96032510
ACCGAGT700.001485162310.86298758
GTATCAA28100.010.830391
GGCGAGG16850.010.648890519