FastQCFastQC Report
Thu 26 May 2016
SRR1512345_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512345_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1645535
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63380.38516348786261No Hit
GTCCTACAGTGGACATTTCTAAATT58210.35374513456110024No Hit
GTCCTAAAGTGTGTATTTCTCATTT55310.3361216868677968No Hit
CTGTAGGACGTGGAATATGGCAAGA52880.3213544531109943No Hit
CTTTAGGACGTGAAATATGGCGAGG49490.3007532504626155No Hit
GGTATCAACGCAGAGTACTTTTTTT37030.22503319589069815No Hit
TATCAACGCAGAGTACTTTTTTTTT36240.22023232565700515No Hit
GTCCTACAGTGTGCATTTCTCATTT35410.21518837338616315No Hit
CTGTAGGACCTGGAATATGGCGAGA24600.14949545284664256No Hit
CTGAAGGACCTGGAATATGGCGAGA22940.13940754830495855No Hit
GTCCTTCAGTGTGCATTTCTCATTT21350.12974503732828532No Hit
TTTCTAAATTTTCCACCTTTTTCAG20350.12366798639956002No Hit
ATTTAGAAATGTCCACTGTAGGACG20320.12348567487169826No Hit
GAATATGGCAAGAAAACTGAAAATC19640.11935328024016505No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18070.10981231028206631No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTCA250.006067244318.9809610
TAGGACC10750.013.1675054
GGTATCA10050.013.1385651
CCAACGT802.847679E-511.88267819
GGCGAGG14650.011.74479119
TAGAAAT8050.011.6832874
AGGACCT19200.011.6771395
GGACCTG19100.011.5393226
ATTTAGA7600.011.374361
GTCTTAT1354.7311914E-911.2586261
CACCTTT7200.011.22733414
CTAGGAC2650.011.1136013
ACCTTTT6950.011.08384315
TCCAACG1553.110472E-1011.03399618
CGCGCCT951.36613235E-510.995687512
TTTAGAA8250.010.939782
CCACCTT7150.010.89848513
CTCTAGG2800.010.8565321
ATCGCGC700.001515524610.8363458
ATAGGAC2900.010.8107273
Produced by FastQC (version 0.11.5)