##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512345_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1645535 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.898828648433486 31.0 30.0 34.0 16.0 34.0 2 29.792816318097152 31.0 30.0 34.0 20.0 34.0 3 30.45592709969706 33.0 31.0 34.0 26.0 34.0 4 34.107816606757076 37.0 35.0 37.0 30.0 37.0 5 34.277301910928664 37.0 35.0 37.0 31.0 37.0 6 34.428650256603476 37.0 35.0 37.0 32.0 37.0 7 34.367244087789075 37.0 35.0 37.0 32.0 37.0 8 34.36792350208291 37.0 35.0 37.0 32.0 37.0 9 35.929853208834814 39.0 37.0 39.0 31.0 39.0 10 35.848644361864075 39.0 37.0 39.0 31.0 39.0 11 35.8691477239925 39.0 37.0 39.0 31.0 39.0 12 35.807101034010216 39.0 37.0 39.0 31.0 39.0 13 35.78260079548597 39.0 37.0 39.0 31.0 39.0 14 36.985067470457935 40.0 38.0 41.0 31.0 41.0 15 36.925061454177516 40.0 38.0 41.0 31.0 41.0 16 36.85524829310832 40.0 38.0 41.0 31.0 41.0 17 36.82522583840514 40.0 38.0 41.0 31.0 41.0 18 36.60820584186906 40.0 37.0 41.0 30.0 41.0 19 36.715437836326785 40.0 38.0 41.0 30.0 41.0 20 36.65542574299544 40.0 38.0 41.0 30.0 41.0 21 36.594118022406086 40.0 38.0 41.0 30.0 41.0 22 36.51569428787598 40.0 37.0 41.0 30.0 41.0 23 36.39239031682705 40.0 37.0 41.0 30.0 41.0 24 36.271319661994426 40.0 37.0 41.0 29.0 41.0 25 36.15436377834565 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3218083461526966 1101 2 0.42149573185143296 1101 3 0.004492893108590579 1101 4 -0.12419609204952309 1101 5 -0.23767398249508176 1101 6 -0.38302180602802594 1101 7 -0.37854541343579484 1101 8 -0.6934775974915723 1101 9 -0.7088292640865177 1101 10 -0.747346341076252 1101 11 -0.8687146003227468 1101 12 -0.8663519105520194 1101 13 -0.8339576124101598 1101 14 -1.060050427265253 1101 15 -1.1646955346070413 1101 16 -1.248114776266391 1101 17 -1.346719612663449 1101 18 -1.1048049068804957 1101 19 -1.207379631743791 1101 20 -1.3770096442522402 1101 21 -1.3518609061203222 1101 22 -1.2270601875773224 1101 23 -1.5040467360347165 1101 24 -1.7332200459784914 1101 25 -1.4999132978088383 1102 1 1.0716078402093672 1102 2 0.236857745332113 1102 3 -0.3085369856187299 1102 4 -0.2154346506791356 1102 5 -0.4443400532135229 1102 6 -0.6043770256989944 1102 7 -0.5493915150989892 1102 8 -0.7788229522701329 1102 9 -0.7619148834660905 1102 10 -0.8653323925461294 1102 11 -0.8454625944278789 1102 12 -1.0726274124016868 1102 13 -0.843723087291778 1102 14 -1.0992096557183828 1102 15 -1.2240365216977978 1102 16 -1.466057635414309 1102 17 -1.2407463735233577 1102 18 -1.2646006247419024 1102 19 -1.3972750511855168 1102 20 -1.6419707293904011 1102 21 -1.6318194180988783 1102 22 -1.5668074832534131 1102 23 -1.64857517947366 1102 24 -1.7261697557461417 1102 25 -1.7380274725765048 1103 1 1.0622880681992406 1103 2 0.2236763013131089 1103 3 -0.22625546551995157 1103 4 -0.3883165773681654 1103 5 -0.5335264361816314 1103 6 -0.6858073254034096 1103 7 -0.6642002555422479 1103 8 -0.6235173236142515 1103 9 -0.9309429332047969 1103 10 -0.9652556891699362 1103 11 -1.021088381728788 1103 12 -1.1623111201056986 1103 13 -1.0989426585012012 1103 14 -1.2652740241939213 1103 15 -1.5397098900361428 1103 16 -1.3539070099388084 1103 17 -1.5099970501344373 1103 18 -1.2087786514072363 1103 19 -1.4605693676524183 1103 20 -1.3874527134510686 1103 21 -1.4694779695899172 1103 22 -1.7894998341183523 1103 23 -1.6510430950126462 1103 24 -1.7438333584351255 1103 25 -1.7821475040061756 1104 1 1.192144416460497 1104 2 0.4087217483217458 1104 3 -0.2365273938900394 1104 4 -0.22249453669950015 1104 5 -0.3615716872330168 1104 6 -0.6218227608053439 1104 7 -0.4314475083723224 1104 8 -0.4840703752715996 1104 9 -0.5930568597359027 1104 10 -0.6689479759910526 1104 11 -0.6946793737246892 1104 12 -0.6062078054134261 1104 13 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-0.36054539775078354 1107 11 -0.3606160566995058 1107 12 -0.454987283035031 1107 13 -0.33784554297370306 1107 14 -0.5049717195878145 1107 15 -0.5742055479495178 1107 16 -0.7486161609770718 1107 17 -0.7150461729050832 1107 18 -0.6138816830925435 1107 19 -0.5539795211891558 1107 20 -0.8454858328765908 1107 21 -0.6466542828421851 1107 22 -0.9033642739321621 1107 23 -0.9768136296439422 1107 24 -0.7832335180884442 1107 25 -0.7792827289895001 1108 1 -0.4652804787127991 1108 2 -0.5017990408093631 1108 3 -0.9226133122294975 1108 4 -0.7732479224401914 1108 5 -0.9267483642804635 1108 6 -0.8637095695424648 1108 7 -0.8399961889583452 1108 8 -0.9144654442810136 1108 9 -1.063171719595367 1108 10 -1.076462414386036 1108 11 -1.0252966825373235 1108 12 -1.1796102320707007 1108 13 -1.2445106926976024 1108 14 -1.4827864316242625 1108 15 -1.4841716970419085 1108 16 -1.44879324755658 1108 17 -1.26820842147594 1108 18 -1.4596795043010573 1108 19 -1.3208435553005842 1108 20 -1.4840643255960941 1108 21 -1.3746507806830124 1108 22 -1.420182552432955 1108 23 -1.3845332194758058 1108 24 -1.3439252439900784 1108 25 -1.5109281337246756 1109 1 0.6352819945810175 1109 2 0.0900380432589607 1109 3 -0.4705406385434401 1109 4 -0.5243113763302532 1109 5 -0.6112004355846068 1109 6 -0.6330133462075622 1109 7 -0.7253842092495688 1109 8 -0.8212170411737816 1109 9 -0.8213164205409029 1109 10 -1.0060115044584634 1109 11 -0.8540552696022203 1109 12 -0.7809834776799249 1109 13 -0.9162389798863089 1109 14 -1.07440712713543 1109 15 -1.10829929507414 1109 16 -1.1031549834858438 1109 17 -1.3489672331466807 1109 18 -1.335239649363558 1109 19 -1.1913740359570895 1109 20 -1.4156910197267578 1109 21 -1.534815290768364 1109 22 -1.562425159613774 1109 23 -1.4902629967325467 1109 24 -1.473326285942072 1109 25 -1.832659570873382 1110 1 0.1671087307845447 1110 2 -0.1894321566637025 1110 3 -0.5167379146536639 1110 4 -0.4153039966178085 1110 5 -0.40867579050885183 1110 6 -0.4663828792191538 1110 7 -0.4164394152236426 1110 8 -0.5768874173658602 1110 9 -0.642186392970558 1110 10 -0.7133195289855223 1110 11 -0.6855491152384232 1110 12 -0.7102751483222818 1110 13 -0.6138299017602833 1110 14 -0.8651496557729317 1110 15 -0.8783593572455217 1110 16 -1.0089249192554774 1110 17 -0.9710705265222614 1110 18 -0.9065396237517689 1110 19 -0.9331459327867506 1110 20 -0.9441627990434753 1110 21 -1.0634609540887539 1110 22 -0.7873237168159619 1110 23 -0.9821020617513554 1110 24 -0.8940649979198483 1110 25 -0.8839045050108965 1111 1 -0.20473493566586853 1111 2 -0.8515783792047316 1111 3 -1.2034060610915986 1111 4 -1.185405532351858 1111 5 -1.292054102604034 1111 6 -1.261406848371351 1111 7 -1.3261073779756885 1111 8 -1.3264606976102584 1111 9 -1.463681413311697 1111 10 -1.6900462692236715 1111 11 -1.6128452103277269 1111 12 -1.663787476845215 1111 13 -1.6042302427361506 1111 14 -1.9418118059948597 1111 15 -2.1706530341371817 1111 16 -2.088504047167426 1111 17 -1.9493478842221137 1111 18 -1.913234303231306 1111 19 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1.517534421322388 1201 17 1.3463690905698016 1201 18 1.4897735648578418 1201 19 1.393966237504685 1201 20 1.490107554879863 1201 21 1.4137850182411213 1201 22 1.4546233641689241 1201 23 1.5951549647342063 1201 24 1.5632484281370296 1201 25 1.4683560364290003 1202 1 2.6123774478549002 1202 2 1.7437180568466175 1202 3 1.4125077631944087 1202 4 1.1824254313701772 1202 5 0.9962769135667386 1202 6 1.0089555220778266 1202 7 0.9996613208116898 1202 8 1.0260510124232383 1202 9 1.1746736513366471 1202 10 1.1605336059840496 1202 11 1.2838658477825575 1202 12 1.2879135672906443 1202 13 1.2502886464417742 1202 14 1.374469679080633 1202 15 1.424395285278564 1202 16 1.4605371476635653 1202 17 1.4947601088682916 1202 18 1.706189851832498 1202 19 1.5163611503593444 1202 20 1.4495742535392466 1202 21 1.5021475544180092 1202 22 1.581327801981125 1202 23 1.620725091233247 1202 24 1.6958717712516815 1202 25 1.7091381671817558 1203 1 2.5923580863298277 1203 2 1.720048752548692 1203 3 1.2052679332428191 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-0.836837111439749 2314 13 -0.6601866365002138 2314 14 -0.743994767495785 2314 15 -0.6332668375167358 2314 16 -0.9875094497448558 2314 17 -0.9356382027109049 2314 18 -1.8386662754624084 2314 19 -1.3285028800716177 2314 20 -1.875022443597679 2314 21 -1.2278052016711314 2314 22 -1.0180512188203181 2314 23 -0.8173441208046555 2314 24 -0.7119196844217655 2314 25 -0.7750461676365461 2315 1 -2.9726238559342413 2315 2 -1.3894340872463822 2315 3 -0.5649429554050442 2315 4 -0.4948504383930441 2315 5 -0.3382246914786933 2315 6 -0.3262400436804356 2315 7 -0.3479568210353392 2315 8 -0.3318249974907843 2315 9 -0.2817656822311392 2315 10 -0.33161230552654786 2315 11 -0.5426570380279827 2315 12 -0.46462803769207994 2315 13 -0.48241054196908095 2315 14 -0.49819323316103237 2315 15 -1.1442854003002765 2315 16 -0.7846164930435009 2315 17 -0.7021792004004226 2315 18 -0.300288856689896 2315 19 -0.43704341149173587 2315 20 -0.3775043756584182 2315 21 -0.4858168257491329 2315 22 -0.36502323449204965 2315 23 -0.475346401351878 2315 24 -0.28289423282533477 2315 25 -0.1713406866125311 2316 1 1.290530496701745 2316 2 0.5996514607733126 2316 3 0.002903146928648681 2316 4 -0.13888875966986802 2316 5 -0.4259500395935305 2316 6 -0.4998089378445485 2316 7 -0.44817121669136384 2316 8 -0.4014325777973866 2316 9 -0.6129608063800163 2316 10 -0.5288691016129974 2316 11 -0.6123979203725298 2316 12 -0.5029124200698121 2316 13 -0.5746143936007613 2316 14 -0.626308542575643 2316 15 -0.5514578656982891 2316 16 -0.3639530827287061 2316 17 -0.4600672882410848 2316 18 -0.41989767917125675 2316 19 -0.4068324234733467 2316 20 -0.443671688070566 2316 21 -0.4555051659552163 2316 22 -0.6669045174398107 2316 23 -0.5774704089562164 2316 24 -0.6220125779667498 2316 25 -0.538766340599004 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49334.0 3 3.0 4 422.0 5 2015.0 6 2492.0 7 2248.0 8 2036.0 9 2079.0 10 1965.0 11 1841.0 12 1849.0 13 1810.0 14 1895.0 15 1869.0 16 2014.0 17 2170.0 18 2349.0 19 2927.0 20 3578.0 21 4256.0 22 5375.0 23 6427.0 24 7459.0 25 8822.0 26 9601.0 27 11063.0 28 12787.0 29 15261.0 30 19056.0 31 25035.0 32 32956.0 33 45148.0 34 63229.0 35 91487.0 36 158623.0 37 268950.0 38 775104.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.11516228513373 19.997999754848003 12.41725357802999 24.46958438198828 2 13.845389604056955 20.2317388336259 43.5139945387166 22.408877023600546 3 18.61419554013307 25.366118033210633 29.913528962067108 26.106157464589185 4 11.858756318257296 17.194742527205385 39.25530514165332 31.691196012884003 5 12.444148642776213 38.42055317756206 36.37086703003951 12.764431149622213 6 30.649068042975536 37.86311477606957 18.750989953042723 12.736827227912167 7 27.322626326940597 31.675808727832482 22.93419301641668 18.06737192881024 8 24.091080899835397 38.71078461257087 20.871913674327867 16.326220813265866 9 26.717915847683066 14.952883817452667 20.875826041180506 37.45337429368376 10 15.318645814678503 28.756153809605607 35.04805484184968 20.877145533866205 11 33.57407737966504 21.900783397981893 26.422669377296387 18.102469845056685 12 24.312457850100795 24.140477123151722 32.705580326818215 18.84148469992927 13 29.61497425910772 22.535421527294975 25.898768129673893 21.950836083923413 14 20.156543492393073 23.465683075890144 27.944238006547117 28.433535425169666 15 22.030606246142813 33.406289562848066 23.34442820936263 21.2186759816465 16 20.7887902840016 27.898044010973454 30.25351707036093 21.05964863466401 17 20.679958086196702 29.398920176418784 29.043189891865072 20.87793184551944 18 20.543552989419762 26.971763718111742 32.48115451774426 20.003528774724238 19 23.507387304059975 26.304583292766043 28.143620387498785 22.0444090156752 20 23.67657221838286 27.41762540757271 29.19514474729731 19.71065762674712 21 22.193104498059213 26.633868635360376 27.659030367607883 23.513996498972524 22 23.083304019075975 29.204060095647655 27.800201325045006 19.912434560231365 23 21.057723649058246 28.84287676949837 29.206661569252233 20.89273801219115 24 23.214487183631054 26.601611597943332 29.998371692080262 20.18552952634535 25 22.051633402355204 27.810485548723868 29.557548255316657 20.58033279360427 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 744.0 1 744.0 2 1177.5 3 1611.0 4 1611.0 5 1611.0 6 2589.5 7 3568.0 8 3568.0 9 3568.0 10 4191.5 11 4815.0 12 4815.0 13 4815.0 14 6509.0 15 8203.0 16 8203.0 17 8203.0 18 14328.0 19 20453.0 20 20453.0 21 20453.0 22 33816.0 23 47179.0 24 47179.0 25 47179.0 26 70091.0 27 93003.0 28 93003.0 29 93003.0 30 121503.0 31 150003.0 32 150003.0 33 150003.0 34 163867.5 35 177732.0 36 177732.0 37 177732.0 38 183994.0 39 190256.0 40 190256.0 41 190256.0 42 196449.0 43 202642.0 44 202642.0 45 202642.0 46 205724.5 47 208807.0 48 208807.0 49 208807.0 50 196667.0 51 184527.0 52 184527.0 53 184527.0 54 160302.0 55 136077.0 56 136077.0 57 136077.0 58 115948.0 59 95819.0 60 95819.0 61 95819.0 62 78689.0 63 61559.0 64 61559.0 65 61559.0 66 47253.5 67 32948.0 68 32948.0 69 32948.0 70 24285.0 71 15622.0 72 15622.0 73 15622.0 74 11021.5 75 6421.0 76 6421.0 77 6421.0 78 4379.0 79 2337.0 80 2337.0 81 2337.0 82 1568.0 83 799.0 84 799.0 85 799.0 86 514.0 87 229.0 88 229.0 89 229.0 90 160.5 91 92.0 92 92.0 93 92.0 94 70.0 95 48.0 96 48.0 97 48.0 98 44.5 99 41.0 100 41.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34857964127168367 2 0.29747164296110384 3 0.3170397469515993 4 0.3069518424099153 5 0.2914553625416658 6 0.24569516904836422 7 0.3561759549325903 8 0.3181336161187699 9 0.25967238618443245 10 0.23512110043238219 11 0.24891600604058864 12 0.24745751381769457 13 0.26568866660387047 14 0.25535768002503745 15 0.3487619527995454 16 0.4054000674552653 17 0.4213827113978129 18 0.11576782019221711 19 0.13217585769977547 20 0.19385792462633733 21 0.19203480934771974 22 0.1471254029844397 23 0.25402072882071786 24 0.35222587182891885 25 0.3914228503191971 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1645535.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.38233427458007 #Duplication Level Percentage of deduplicated Percentage of total 1 83.30787962919571 55.3017151325175 2 11.1193028529201 14.762505577656682 3 2.88888287291968 5.753123656507904 4 1.032267732797862 2.7409736679780234 5 0.5015570745533923 1.6647264690391885 6 0.27473728025693067 1.0942621185422738 7 0.1666308953663206 0.774294345767577 8 0.11655220738535856 0.6189606072876043 9 0.0857459141782367 0.5122812543893248 >10 0.43708976203926914 5.259527648065613 >50 0.033529914691321924 1.5386010344584031 >100 0.02965405398493838 4.00094883854033 >500 0.0034992950598829536 1.6380209653075863 >1k 0.002302167802554575 2.9354294205877856 >5k 3.6834684840873197E-4 1.4046292633541468 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6338 0.38516348786261 No Hit GTCCTACAGTGGACATTTCTAAATT 5821 0.35374513456110024 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5531 0.3361216868677968 No Hit CTGTAGGACGTGGAATATGGCAAGA 5288 0.3213544531109943 No Hit CTTTAGGACGTGAAATATGGCGAGG 4949 0.3007532504626155 No Hit GGTATCAACGCAGAGTACTTTTTTT 3703 0.22503319589069815 No Hit TATCAACGCAGAGTACTTTTTTTTT 3624 0.22023232565700515 No Hit GTCCTACAGTGTGCATTTCTCATTT 3541 0.21518837338616315 No Hit CTGTAGGACCTGGAATATGGCGAGA 2460 0.14949545284664256 No Hit CTGAAGGACCTGGAATATGGCGAGA 2294 0.13940754830495855 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2135 0.12974503732828532 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2035 0.12366798639956002 No Hit ATTTAGAAATGTCCACTGTAGGACG 2032 0.12348567487169826 No Hit GAATATGGCAAGAAAACTGAAAATC 1964 0.11935328024016505 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1807 0.10981231028206631 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.077050928725308E-5 2 0.0 0.0 0.0 0.0 6.077050928725308E-5 3 0.0 0.0 0.0 0.0 6.077050928725308E-5 4 0.0 0.0 0.0 0.0 6.077050928725308E-5 5 0.0 0.0 0.0 0.0 6.077050928725308E-5 6 0.0 0.0 0.0 0.0 1.2154101857450616E-4 7 0.0 0.0 0.0 0.0 1.2154101857450616E-4 8 0.0 0.0 0.0 0.0 1.2154101857450616E-4 9 0.0 0.0 0.0 6.077050928725308E-5 1.2154101857450616E-4 10 0.0 0.0 0.0 6.077050928725308E-5 1.2154101857450616E-4 11 0.0 0.0 0.0 6.077050928725308E-5 1.2154101857450616E-4 12 0.0 0.0 0.0 6.077050928725308E-5 2.4308203714901232E-4 13 0.0 0.0 0.0 6.077050928725308E-5 2.4308203714901232E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGTCA 25 0.0060672443 18.98096 10 TAGGACC 1075 0.0 13.167505 4 GGTATCA 1005 0.0 13.138565 1 CCAACGT 80 2.847679E-5 11.882678 19 GGCGAGG 1465 0.0 11.744791 19 TAGAAAT 805 0.0 11.683287 4 AGGACCT 1920 0.0 11.677139 5 GGACCTG 1910 0.0 11.539322 6 ATTTAGA 760 0.0 11.37436 1 GTCTTAT 135 4.7311914E-9 11.258626 1 CACCTTT 720 0.0 11.227334 14 CTAGGAC 265 0.0 11.113601 3 ACCTTTT 695 0.0 11.083843 15 TCCAACG 155 3.110472E-10 11.033996 18 CGCGCCT 95 1.36613235E-5 10.9956875 12 TTTAGAA 825 0.0 10.93978 2 CCACCTT 715 0.0 10.898485 13 CTCTAGG 280 0.0 10.856532 1 ATCGCGC 70 0.0015155246 10.836345 8 ATAGGAC 290 0.0 10.810727 3 >>END_MODULE