FastQCFastQC Report
Thu 26 May 2016
SRR1512345_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512345_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1645535
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74980.4556572786358236No Hit
GTCCTACAGTGGACATTTCTAAATT59040.35878908683194216No Hit
CTGTAGGACGTGGAATATGGCAAGA56070.34074024557362803No Hit
GTCCTAAAGTGTGTATTTCTCATTT55220.3355747522842115No Hit
CTTTAGGACGTGAAATATGGCGAGG52030.3161889598215778No Hit
TATCAACGCAGAGTACTTTTTTTTT46490.2825220976764396No Hit
GGTATCAACGCAGAGTACTTTTTTT43930.2669648472989028No Hit
GTCCTACAGTGTGCATTTCTCATTT33690.20473584578875564No Hit
CTGTAGGACCTGGAATATGGCGAGA28310.1720413117922135No Hit
CTGAAGGACCTGGAATATGGCGAGA26490.1609810791019334No Hit
TTTCTAAATTTTCCACCTTTTTCAG22450.13642979334988317No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21760.1322366282090627No Hit
ATTTAGAAATGTCCACTGTAGGACG21440.1302919719118706No Hit
GTCCTTCAGTGTGCATTTCTCATTT20710.12585572473390114No Hit
GAATATGGCAAGAAAACTGAAAATC20680.12567341320603936No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18630.11321545880215249No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGCG250.006016013719.0088547
GGTATCA12300.014.5337231
TAGGACC10800.012.5818854
CACCTTT8100.012.3085714
AACCGTG550.003057888712.0965427
CCACCTT7750.012.01046313
ACCTTTT8100.011.72173515
GGCGAGG15450.011.6765719
TAGAAAT7900.011.4269414
GTTCTAA1803.6379788E-1211.0935591
TGGACGG600.0058608711.0871495
TTTAGAA8600.011.0493062
AATGTCC7250.010.880938
ATGTCCA7600.010.8790749
TGTCCAC8050.010.73825510
AAATGTC7000.010.7264247
TAGAACG803.7621238E-410.6902034
GCGCCAC803.7780148E-410.68532813
CCAACTT1605.456968E-1010.68532813
TGGCGAG33900.010.61523518
Produced by FastQC (version 0.11.5)