FastQCFastQC Report
Thu 26 May 2016
SRR1512344_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512344_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1820368
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64910.356576252713737No Hit
GTCCTACAGTGGACATTTCTAAATT46490.2553879215631125No Hit
CTGTAGGACGTGGAATATGGCAAGA41950.23044790943369692No Hit
GTCCTAAAGTGTGTATTTCTCATTT41870.23000843785432398No Hit
TATCAACGCAGAGTACTTTTTTTTT38120.20940820757121636No Hit
CTTTAGGACGTGAAATATGGCGAGG36590.20100331361570847No Hit
GGTATCAACGCAGAGTACTTTTTTT36200.19886088966626528No Hit
GTCCTACAGTGTGCATTTCTCATTT21310.11706424195547276No Hit
CTGAAGGACCTGGAATATGGCGAGA19350.10629718826083516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11250.013.937141
TAGGACC7000.013.7105094
CGAGTAA550.00309304312.07820310
GGCGAGG11200.011.88218319
GTATAAG2450.011.6358321
GCCGGTT752.0913141E-411.38990511
CGTATCA600.005864354311.0863631
GTCCTAA12450.010.9909531
CGACTAA1752.0008883E-1110.86761317
GATATAC4650.010.8309151
CTAGGAC3050.010.5930583
TAGGACA7100.010.5730194
GCGACTA1803.45608E-1110.56544316
GTATCAA26000.010.5625011
CCAACGA909.457988E-510.5619419
TGTAGGA23250.010.5448172
ACTGTTC4550.010.4188198
TTAGGAC18050.010.3712543
GTCGAGG1106.0023667E-610.36990519
TAAGCGA1659.276846E-1010.36417813
Produced by FastQC (version 0.11.5)