FastQCFastQC Report
Thu 26 May 2016
SRR1512344_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512344_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1820368
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT77970.428319988046373No Hit
TATCAACGCAGAGTACTTTTTTTTT49270.27065955894632293No Hit
GTCCTACAGTGGACATTTCTAAATT47060.2585191565661449No Hit
CTGTAGGACGTGGAATATGGCAAGA46330.2545089784043666No Hit
GGTATCAACGCAGAGTACTTTTTTT45430.24956492313642076No Hit
GTCCTAAAGTGTGTATTTCTCATTT42500.23346927654188604No Hit
CTTTAGGACGTGAAATATGGCGAGG39910.21924138415968641No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23090.12684248459652114No Hit
GTCCTACAGTGTGCATTTCTCATTT20740.11393300695244038No Hit
CTGAAGGACCTGGAATATGGCGAGA20630.11332873353080257No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19870.10915375352675942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13200.015.0551251
TAGGACC6300.012.6703334
ACCGTGC550.00305899612.0960718
GCCGGTT1157.082053E-811.56443111
CGGTTTC1251.8355422E-811.39859813
TCCAACG1751.8189894E-1211.39296218
GAGCGTC600.005863670711.086549
CTAGGAC3300.010.9425593
CACCTTT7400.010.77949514
ACCTTTT7250.010.7396715
TTAGGAC15200.010.6905933
GCCCCGT1002.4095429E-510.44584514
GGCGAGG12550.010.44005419
CCACCTT7600.010.37372513
GTATCAA28600.010.372911
CCAACGT1106.0886014E-610.35752419
AGGACGT26500.010.291945
GACGTGG13250.010.1854787
GTAAGAC2250.010.1362673
GGACGTG26450.010.1328686
Produced by FastQC (version 0.11.5)