FastQCFastQC Report
Thu 26 May 2016
SRR1512343_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512343_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2146670
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT73160.34080692421285064No Hit
GTATCAACGCAGAGTACTTTTTTTT72200.33633488146757534No Hit
GTCCTAAAGTGTGTATTTCTCATTT67580.31481317575593826No Hit
CTGTAGGACGTGGAATATGGCAAGA64130.2987417721401054No Hit
CTTTAGGACGTGAAATATGGCGAGG56230.2619405870487779No Hit
TATCAACGCAGAGTACTTTTTTTTT39910.1859158603790988No Hit
GTCCTACAGTGTGCATTTCTCATTT39820.18549660637172924No Hit
GGTATCAACGCAGAGTACTTTTTTT39800.185403438814536No Hit
CTGAAGGACCTGGAATATGGCGAGA29510.13746873063861703No Hit
CTGTAGGACCTGGAATATGGCGAGA28660.13350910945790456No Hit
GTCCTTCAGTGTGCATTTCTCATTT25660.11953397587891944No Hit
ATTTAGAAATGTCCACTGTAGGACG23580.10984454993082308No Hit
TTTCTAAATTTTCCACCTTTTTCAG23120.1077016961153787No Hit
GAATATGGCAAGAAAACTGAAAATC22580.10518617207116138No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCAC400.0053073814.23887810
GGTATCA11550.013.243551
TAGGACC11100.012.9245224
GGCGAGG16400.011.24297119
TGTAGGA37000.010.8106322
AATGTCC8550.010.7587958
GTATCAA26550.010.5206691
AAATGTC8700.010.4829147
GTCCTAA19250.010.3645171
GACGTGG18250.010.3590577
GTAGGAC37350.010.327763
AGGACCT22750.010.3144395
CTGTAGG35300.010.2544131
GGACGTG38300.010.0949766
GCGCCTT856.58569E-410.06038613
AGGACGT39100.010.0589735
TAGAAAT9450.010.0537644
CGGTTTC951.6419023E-410.00155313
GACAGCG951.6442312E-410.0001497
GGACCTG23750.09.999686
Produced by FastQC (version 0.11.5)