##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512343_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2146670 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.16532070602375 31.0 30.0 34.0 16.0 34.0 2 30.054595722677448 31.0 30.0 34.0 24.0 34.0 3 30.725912692682154 33.0 31.0 34.0 27.0 34.0 4 34.38460406117382 37.0 35.0 37.0 32.0 37.0 5 34.55913810692841 37.0 35.0 37.0 32.0 37.0 6 34.71443491547373 37.0 35.0 37.0 32.0 37.0 7 34.65377538233636 37.0 35.0 37.0 32.0 37.0 8 34.6505243004281 37.0 35.0 37.0 32.0 37.0 9 36.25196606837567 39.0 37.0 39.0 32.0 39.0 10 36.17959770248804 39.0 37.0 39.0 32.0 39.0 11 36.20761598196276 39.0 37.0 39.0 32.0 39.0 12 36.15569416817676 39.0 37.0 39.0 32.0 39.0 13 36.131754764355954 39.0 37.0 39.0 32.0 39.0 14 37.38223527603218 40.0 38.0 41.0 32.0 41.0 15 37.320273726283034 40.0 38.0 41.0 32.0 41.0 16 37.25338174940722 40.0 38.0 41.0 32.0 41.0 17 37.21738087363218 40.0 38.0 41.0 32.0 41.0 18 37.0003684776887 40.0 38.0 41.0 31.0 41.0 19 37.11541783320212 40.0 38.0 41.0 31.0 41.0 20 37.0564595396591 40.0 38.0 41.0 31.0 41.0 21 36.99887080920682 40.0 38.0 41.0 31.0 41.0 22 36.93045461109532 40.0 38.0 41.0 31.0 41.0 23 36.8124676825036 40.0 38.0 41.0 31.0 41.0 24 36.70036102428412 40.0 38.0 41.0 30.0 41.0 25 36.58569738245748 40.0 38.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.274796134916258 1101 2 0.3272326491691011 1101 3 -0.09269894550430635 1101 4 -0.1919356364404976 1101 5 -0.37680363488958335 1101 6 -0.44306906436015936 1101 7 -0.33100449316452796 1101 8 -0.5985027573622617 1101 9 -0.564994045115796 1101 10 -0.5642378816617963 1101 11 -0.5839996534521745 1101 12 -0.7237594891990256 1101 13 -0.7641963099988942 1101 14 -1.0550160072395371 1101 15 -1.1732237508915802 1101 16 -1.1041047763080556 1101 17 -1.2233245310640726 1101 18 -1.0477862023859075 1101 19 -1.2157507555596467 1101 20 -1.181587810869928 1101 21 -1.258470394024485 1101 22 -1.2525688489502471 1101 23 -1.4636523608913734 1101 24 -1.5166079381979287 1101 25 -1.5648497338251985 1102 1 1.0708218256787916 1102 2 0.2383387313278149 1102 3 -0.13014236978983362 1102 4 -0.2872443422755211 1102 5 -0.3654482603763114 1102 6 -0.43741939555259535 1102 7 -0.4760308592435294 1102 8 -0.5317520353483829 1102 9 -0.6406467312947939 1102 10 -0.6610883206174947 1102 11 -0.6086530149476417 1102 12 -0.8134566025803451 1102 13 -0.667099452375723 1102 14 -1.0240663973425583 1102 15 -1.1041769528974186 1102 16 -1.0154105797191164 1102 17 -1.1061183296473445 1102 18 -0.982202915749987 1102 19 -0.9957197197391352 1102 20 -1.1435840176029757 1102 21 -1.2706526719962383 1102 22 -1.248623276583622 1102 23 -1.4366382654562315 1102 24 -1.373157071062849 1102 25 -1.576871085320235 1103 1 1.3708018431276372 1103 2 0.5635300295078061 1103 3 0.05043518290132809 1103 4 -0.04631994080077817 1103 5 -0.17807390607816131 1103 6 -0.32451980434044714 1103 7 -0.4346104712539258 1103 8 -0.30667263489782925 1103 9 -0.6461778194278764 1103 10 -0.5425578424421076 1103 11 -0.5393222207882005 1103 12 -0.5399975546201716 1103 13 -0.47172546993074604 1103 14 -0.7507625160566818 1103 15 -0.7389863747272472 1103 16 -0.7406142730049581 1103 17 -0.7657628498599109 1103 18 -0.7082214049643056 1103 19 -0.8589657017539309 1103 20 -1.001057617052851 1103 21 -0.8719572568866454 1103 22 -1.1138816404888914 1103 23 -1.0956634860788625 1103 24 -1.0045131799474802 1103 25 -1.2389504235674096 1104 1 1.3088466914309187 1104 2 0.548234936984727 1104 3 -0.07193245709075669 1104 4 -0.09714614822812706 1104 5 -0.30576953286891495 1104 6 -0.3131754080190703 1104 7 -0.3272812954626403 1104 8 -0.38984625252697214 1104 9 -0.39599792364167286 1104 10 -0.5183154802507417 1104 11 -0.43423147607862234 1104 12 -0.4876346933011817 1104 13 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21 -0.7124879966876847 1108 22 -0.8683930047292563 1108 23 -0.637255206503248 1108 24 -0.8426778928231897 1108 25 -0.9769447243626246 1109 1 0.6895411950796451 1109 2 0.13838199119493666 1109 3 -0.31959540009000165 1109 4 -0.35042893596965285 1109 5 -0.5104065967793971 1109 6 -0.4574599855239825 1109 7 -0.4939808704835329 1109 8 -0.5896875132880552 1109 9 -0.6625190964257044 1109 10 -0.5899330806637693 1109 11 -0.5729167124887553 1109 12 -0.6813855506099458 1109 13 -0.7319193572040135 1109 14 -0.8838622384762047 1109 15 -0.8403471763815418 1109 16 -1.0091130261370225 1109 17 -0.8757692974530613 1109 18 -0.9743506516595204 1109 19 -0.9387375960763649 1109 20 -0.9307467307459802 1109 21 -0.9580739998033891 1109 22 -1.0458724024131598 1109 23 -1.1018390082413703 1109 24 -1.1611509781592986 1109 25 -1.2777174548602517 1110 1 0.5071235637538649 1110 2 0.061036201938172496 1110 3 -0.3048560378234235 1110 4 -0.31220689912970556 1110 5 -0.24719135085270239 1110 6 -0.2616126220811168 1110 7 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-0.45331632004259603 2314 9 -0.5140712495260615 2314 10 -0.4854173923386469 2314 11 -1.3984795566938288 2314 12 -1.0331874426196208 2314 13 -0.7704878729516764 2314 14 -0.6856335847308372 2314 15 -0.7621987068260765 2314 16 -0.8817436497172579 2314 17 -1.0525039426709455 2314 18 -2.1751588252223613 2314 19 -1.6726676977646946 2314 20 -2.0582599263855244 2314 21 -1.6751944387215545 2314 22 -1.1470163893315828 2314 23 -1.0863282410662336 2314 24 -1.020334414237439 2314 25 -0.9090264829263432 2315 1 -2.767824314179009 2315 2 -1.3445026898324244 2315 3 -0.5730673780310092 2315 4 -0.546168613695059 2315 5 -0.3610780335524737 2315 6 -0.31447375878643413 2315 7 -0.3330900974084088 2315 8 -0.30274300153056544 2315 9 -0.3834738067610246 2315 10 -0.32025125278308053 2315 11 -0.5884978730583086 2315 12 -0.6460362132743072 2315 13 -0.5463742040472965 2315 14 -0.6264134347520738 2315 15 -1.160662890488993 2315 16 -0.9499699023890074 2315 17 -0.9046267620065862 2315 18 -0.653505263500648 2315 19 -0.7475798377484395 2315 20 -0.7309773901156262 2315 21 -0.6060868463171971 2315 22 -0.5717462343146806 2315 23 -0.7809403486829609 2315 24 -0.5352657392298283 2315 25 -0.35610400313701973 2316 1 1.1229551443655907 2316 2 0.4214815901922364 2316 3 -0.14846629643294662 2316 4 -0.30122204895271665 2316 5 -0.5207919046843017 2316 6 -0.6407230233044388 2316 7 -0.5619592015385919 2316 8 -0.572093503533651 2316 9 -0.6723254234149181 2316 10 -0.7602300615249433 2316 11 -0.8041365058470902 2316 12 -0.7227982640442505 2316 13 -0.7188883921103297 2316 14 -0.8311645898397302 2316 15 -0.8168698896990136 2316 16 -0.6819965977482596 2316 17 -0.6995708653749233 2316 18 -0.6653163424966877 2316 19 -0.6645940269835648 2316 20 -0.6145790132159732 2316 21 -0.6209500290847672 2316 22 -0.7295320291392358 2316 23 -0.7824206893371226 2316 24 -0.8653371659594171 2316 25 -0.7535268273410054 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 53428.0 3 5.0 4 461.0 5 2240.0 6 2567.0 7 2462.0 8 2290.0 9 2222.0 10 2145.0 11 2151.0 12 2204.0 13 2189.0 14 2313.0 15 2200.0 16 2371.0 17 2564.0 18 2798.0 19 3402.0 20 4226.0 21 4960.0 22 6313.0 23 7578.0 24 8918.0 25 10491.0 26 11838.0 27 13193.0 28 15472.0 29 18502.0 30 23659.0 31 30851.0 32 41140.0 33 56287.0 34 78809.0 35 115753.0 36 202194.0 37 343523.0 38 1062951.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.3516349450157 19.63051186862478 12.357654564168916 24.660198622190602 2 14.115524292170411 19.954157694354414 42.93090809533443 22.999409918140742 3 18.606386697246847 24.979578943039737 29.616140205276015 26.797894154437397 4 12.070700014625205 16.910100783079248 38.83281522105319 32.18638398124236 5 12.820729643148635 38.159778852411456 35.95105936964341 13.0684321347965 6 31.236226955031054 37.332254294014554 18.456895943842635 12.97462280711176 7 27.90350976430473 31.552069591298842 22.58926342539308 17.955157219003347 8 24.5094305323913 38.011814510042704 20.689411849371307 16.789343108194686 9 26.77930337793375 15.109419609268057 20.68849563391076 37.422781378887436 10 15.512180043601711 28.547401803928636 34.57123659742284 21.36918155504681 11 33.68957011552439 22.04476021380745 26.011573920572197 18.254095750095967 12 24.437421110069753 24.163585785779894 32.01169497247871 19.387298131671642 13 29.369017176352447 22.353125255426722 25.80852628997793 22.469331278242898 14 20.446969067196207 23.061877750181907 27.703105983405734 28.788047199216148 15 22.27171950280631 32.63330259100345 23.250503555623904 21.844474350566333 16 21.310652707199633 27.852317132774733 29.353139126184757 21.48389103384087 17 21.10393787051586 29.180031706544657 28.563349236502848 21.152681186436634 18 21.027244947073463 26.848511079686432 31.616423457071114 20.50782051616899 19 23.61052418493445 26.3681132570065 27.944977886487386 22.07638467157166 20 23.850844824962874 27.174417313007627 28.783556600612158 20.19118126141734 21 22.41690971488099 26.61974190916648 27.446782592380494 23.51656578357204 22 23.29903409769497 28.83258502030336 27.465047623313033 20.40333325868864 23 21.419925185984283 28.508576525584807 28.7930285666251 21.27846972180581 24 23.35158927679912 26.404161906346822 29.474311101471006 20.769937715383048 25 22.298893898319825 27.60633947356238 29.016919572523708 21.077847055594084 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 872.0 1 872.0 2 1427.0 3 1982.0 4 1982.0 5 1982.0 6 3040.0 7 4098.0 8 4098.0 9 4098.0 10 4798.0 11 5498.0 12 5498.0 13 5498.0 14 7760.0 15 10022.0 16 10022.0 17 10022.0 18 17628.0 19 25234.0 20 25234.0 21 25234.0 22 40791.5 23 56349.0 24 56349.0 25 56349.0 26 83751.0 27 111153.0 28 111153.0 29 111153.0 30 145454.0 31 179755.0 32 179755.0 33 179755.0 34 200067.0 35 220379.0 36 220379.0 37 220379.0 38 232598.0 39 244817.0 40 244817.0 41 244817.0 42 254220.5 43 263624.0 44 263624.0 45 263624.0 46 270740.0 47 277856.0 48 277856.0 49 277856.0 50 265296.5 51 252737.0 52 252737.0 53 252737.0 54 221418.5 55 190100.0 56 190100.0 57 190100.0 58 162641.0 59 135182.0 60 135182.0 61 135182.0 62 110770.0 63 86358.0 64 86358.0 65 86358.0 66 66264.0 67 46170.0 68 46170.0 69 46170.0 70 33702.0 71 21234.0 72 21234.0 73 21234.0 74 14821.0 75 8408.0 76 8408.0 77 8408.0 78 5778.5 79 3149.0 80 3149.0 81 3149.0 82 2119.5 83 1090.0 84 1090.0 85 1090.0 86 732.5 87 375.0 88 375.0 89 375.0 90 248.0 91 121.0 92 121.0 93 121.0 94 90.5 95 60.0 96 60.0 97 60.0 98 53.5 99 47.0 100 47.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3415522646703965 2 0.29296538359412483 3 0.3131361597264601 4 0.30414549045731293 5 0.2887728435204293 6 0.24498409163960927 7 0.3518006959616522 8 0.3148597595345349 9 0.2566300362887635 10 0.2334313145476482 11 0.247080361676457 12 0.2439592485104837 13 0.2629654301779034 14 0.2537884257943699 15 0.34392803737882394 16 0.39945590146599147 17 0.41650556443235337 18 0.11953397587891944 19 0.12726688312595788 20 0.19080715713174357 21 0.19057423823876052 22 0.14822958349443557 23 0.24968905327786756 24 0.3474218207735702 25 0.38473542742946054 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2146670.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.71149115866152 #Duplication Level Percentage of deduplicated Percentage of total 1 82.30796875049671 54.908873295861504 2 11.842475097596264 15.800583455399247 3 3.077809116705612 6.159757070314628 4 1.1195844752833946 2.987565992969715 5 0.520625230013433 1.7365842714508626 6 0.28273117923996277 1.1316851138486832 7 0.17651886171638217 0.8243085537910597 8 0.11402069906400215 0.6085192686010069 9 0.08432377505571387 0.5062828296684797 >10 0.4136831092289209 4.968566851787204 >50 0.030269579055362934 1.390521100405206 >100 0.024584803684495487 3.4123167222606723 >500 0.0026675619308934935 1.1649629426217631 >1k 0.0023867659381677175 2.8386020181730225 >5k 3.509949909070173E-4 1.56087051284698 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 7316 0.34080692421285064 No Hit GTATCAACGCAGAGTACTTTTTTTT 7220 0.33633488146757534 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6758 0.31481317575593826 No Hit CTGTAGGACGTGGAATATGGCAAGA 6413 0.2987417721401054 No Hit CTTTAGGACGTGAAATATGGCGAGG 5623 0.2619405870487779 No Hit TATCAACGCAGAGTACTTTTTTTTT 3991 0.1859158603790988 No Hit GTCCTACAGTGTGCATTTCTCATTT 3982 0.18549660637172924 No Hit GGTATCAACGCAGAGTACTTTTTTT 3980 0.185403438814536 No Hit CTGAAGGACCTGGAATATGGCGAGA 2951 0.13746873063861703 No Hit CTGTAGGACCTGGAATATGGCGAGA 2866 0.13350910945790456 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2566 0.11953397587891944 No Hit ATTTAGAAATGTCCACTGTAGGACG 2358 0.10984454993082308 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2312 0.1077016961153787 No Hit GAATATGGCAAGAAAACTGAAAATC 2258 0.10518617207116138 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.658377859661709E-5 0.0 4 0.0 0.0 0.0 4.658377859661709E-5 0.0 5 0.0 0.0 0.0 4.658377859661709E-5 0.0 6 0.0 0.0 0.0 4.658377859661709E-5 0.0 7 0.0 0.0 0.0 4.658377859661709E-5 0.0 8 0.0 0.0 0.0 4.658377859661709E-5 0.0 9 0.0 0.0 0.0 4.658377859661709E-5 0.0 10 0.0 0.0 0.0 4.658377859661709E-5 0.0 11 0.0 0.0 0.0 4.658377859661709E-5 0.0 12 0.0 0.0 0.0 9.316755719323418E-5 0.0 13 0.0 0.0 0.0 9.316755719323418E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTCAC 40 0.00530738 14.238878 10 GGTATCA 1155 0.0 13.24355 1 TAGGACC 1110 0.0 12.924522 4 GGCGAGG 1640 0.0 11.242971 19 TGTAGGA 3700 0.0 10.810632 2 AATGTCC 855 0.0 10.758795 8 GTATCAA 2655 0.0 10.520669 1 AAATGTC 870 0.0 10.482914 7 GTCCTAA 1925 0.0 10.364517 1 GACGTGG 1825 0.0 10.359057 7 GTAGGAC 3735 0.0 10.32776 3 AGGACCT 2275 0.0 10.314439 5 CTGTAGG 3530 0.0 10.254413 1 GGACGTG 3830 0.0 10.094976 6 GCGCCTT 85 6.58569E-4 10.060386 13 AGGACGT 3910 0.0 10.058973 5 TAGAAAT 945 0.0 10.053764 4 CGGTTTC 95 1.6419023E-4 10.001553 13 GACAGCG 95 1.6442312E-4 10.000149 7 GGACCTG 2375 0.0 9.99968 6 >>END_MODULE