FastQCFastQC Report
Thu 26 May 2016
SRR1512343_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512343_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2146670
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT83350.3882757946028034No Hit
GTCCTACAGTGGACATTTCTAAATT73120.34062058909846415No Hit
CTGTAGGACGTGGAATATGGCAAGA70170.3268783744124621No Hit
GTCCTAAAGTGTGTATTTCTCATTT69420.3233845910177158No Hit
CTTTAGGACGTGAAATATGGCGAGG62140.28947160019937856No Hit
GGTATCAACGCAGAGTACTTTTTTT50070.23324497943326172No Hit
TATCAACGCAGAGTACTTTTTTTTT49110.2287729366879865No Hit
GTCCTACAGTGTGCATTTCTCATTT40720.18968914644542478No Hit
CTGAAGGACCTGGAATATGGCGAGA31800.14813641593724233No Hit
CTGTAGGACCTGGAATATGGCGAGA29440.1371426441884407No Hit
ATTTAGAAATGTCCACTGTAGGACG26550.12367993217401836No Hit
GTCCTTCAGTGTGCATTTCTCATTT26170.12190974858734692No Hit
TTTCTAAATTTTCCACCTTTTTCAG25900.12065198656523825No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25190.11734453828487842No Hit
GAATATGGCAAGAAAACTGAAAATC22800.10621101520028695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13900.013.3399061
TAGGACC10750.012.9932474
CCAACGA853.9685638E-612.28765119
TCCAACG1201.0099029E-811.86875318
GGACCGC657.9983094E-411.6970576
CACCTTT8850.011.58847114
TGGACCG752.0693924E-411.4030365
GTCGAGG1251.8471837E-811.39400519
AGCGTAC1303.2228854E-810.9718751
CGCAGCG700.001486757810.8615537
ACCTTTT9550.010.83748115
GTCCTAA19200.010.6975781
AATGTCC8800.010.6918418
CCACCTT9700.010.57668313
AGGACCT21150.010.5583665
GGCGAGG16650.010.55000319
GTATCAA29900.010.526641
TGTAGGA35900.010.4279882
TTTAGAA10100.010.3483242
ATTTAGA9650.010.3465351
Produced by FastQC (version 0.11.5)