FastQCFastQC Report
Thu 26 May 2016
SRR1512342_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512342_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1488945
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73740.4952499924443146No Hit
TATCAACGCAGAGTACTTTTTTTTT47360.3180775649872897No Hit
GGTATCAACGCAGAGTACTTTTTTT44430.2983992021196216No Hit
GTCCTAAAGTGTGTATTTCTCATTT26140.1755605479047245No Hit
GTCCTACAGTGGACATTTCTAAATT24690.16582210894290925No Hit
CTGTAGGACGTGGAATATGGCAAGA24040.1614566018220955No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22630.15198680945233034No Hit
CTTTAGGACGTGAAATATGGCGAGG22120.1485615654036919No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21690.14567361453915356No Hit
GAGTACTTTTTTTTTTTTTTTTTTT16520.11095104251668128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3550.014.1845874
GGTATCA10100.014.1094371
CTTATAC1302.6066118E-911.6943233
CTAATAC1302.6066118E-911.6943233
AGGACCT7950.010.8749875
GCCGGTT700.001497597710.851686511
GTATCAA24350.010.6903371
AGTACCG803.767974E-410.6882595
AGACCGT803.7691495E-410.6878996
GGCGAGG6000.010.45391919
AAGTCGT1002.3962852E-510.4507437
TAGGACA4500.010.3455294
GTAGGAC14150.010.2738553
ATAGTAC856.5821165E-410.0605573
TGTAGGA14450.010.0602182
CCTTATA1356.426726E-79.8532182
TTAGGAC9000.09.8183583
CTGTAGG14200.09.7010671
GTCCTAC11850.09.7007841
CACCTTT5100.09.69350714