FastQCFastQC Report
Thu 26 May 2016
SRR1512342_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512342_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1488945
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84270.565971207801497No Hit
TATCAACGCAGAGTACTTTTTTTTT58890.39551494514572394No Hit
GGTATCAACGCAGAGTACTTTTTTT56480.37932898797470693No Hit
CTGTAGGACGTGGAATATGGCAAGA26680.1791872768974005No Hit
GTCCTAAAGTGTGTATTTCTCATTT26160.17569487120074953No Hit
GTCCTACAGTGGACATTTCTAAATT25210.16931451463956024No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25050.16823992827135992No Hit
GTACTTTTTTTTTTTTTTTTTTTTT24850.16689669531110954No Hit
CTTTAGGACGTGAAATATGGCGAGG23690.15910594414165735No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20080.13486058920913802No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTGG250.006050285418.99002515
GGTATCA12750.014.3999281
CCGCGCG400.005266770714.2549679
GTCGAGG551.9710598E-413.80907219
TAGGACC3900.012.1820954
GTGTCGA658.031287E-411.69088113
TCGAAAC658.065311E-411.68499216
GGCGAGG5950.011.64777819
TAATACT1552.5465852E-1111.6476554
CCTACAC1251.825174E-811.4024413
CTATTAG855.2491716E-511.1916541
TTAGCTA700.001488676310.85946754
TGTGTCG803.7723815E-410.686913512
TTAGGCA1355.666334E-810.5578164
TGGCGAG13700.010.39460218
CCACCTT5050.010.34527213
ATTATGC1201.5188834E-610.2938693
GTATCAA28200.010.2887811
AGGACCT9150.010.2812355
TAGGCTG1303.8263715E-710.2333035
Produced by FastQC (version 0.11.5)