FastQCFastQC Report
Thu 26 May 2016
SRR1512341_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512341_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816397
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT77520.42677894755386625No Hit
TATCAACGCAGAGTACTTTTTTTTT44120.24289844125485782No Hit
GTCCTACAGTGGACATTTCTAAATT42740.2353009832101683No Hit
GGTATCAACGCAGAGTACTTTTTTT42660.2348605508597515No Hit
GTCCTAAAGTGTGTATTTCTCATTT40640.22373963401172758No Hit
CTGTAGGACGTGGAATATGGCAAGA40170.22115209395302898No Hit
CTTTAGGACGTGAAATATGGCGAGG35220.19390034227099032No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21960.12089868018940794No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21130.11632919455383377No Hit
GTCCTACAGTGTGCATTTCTCATTT19970.10994292547279036No Hit
CTGAAGGACCTGGAATATGGCGAGA18660.1027308457347155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11550.014.149021
CCAACGA1351.8189894E-1213.37913919
TCCAACG2300.012.80813418
AACCGTG550.003065163312.0928127
ATACGGC657.956441E-411.7041915
TAGGACC6050.011.4642954
GTATCAA29300.011.4144491
AAATGTC5850.011.3693097
AATGTCC6050.010.9691778
GGCGAGG10000.010.83710319
GTATAAC1502.1282176E-910.7680631
ATCCCGT1252.272318E-710.629024510
GTGTAGG2950.010.6284971
GTATTAG1701.382432E-1010.6190241
TGTAGAA7450.010.5855582
GTAGAAC6200.010.5742443
TAGCACC1803.45608E-1110.5569244
GGACGTG23450.010.4125766
AGGACGT24300.010.3614255
TATGTCG1752.3101165E-1010.32591116
Produced by FastQC (version 0.11.5)