FastQCFastQC Report
Thu 26 May 2016
SRR1512341_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512341_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816397
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT90410.49774361001477097No Hit
TATCAACGCAGAGTACTTTTTTTTT56050.30857791551076114No Hit
GGTATCAACGCAGAGTACTTTTTTT53400.2939885939032051No Hit
GTCCTACAGTGGACATTTCTAAATT44140.24300854934246205No Hit
CTGTAGGACGTGGAATATGGCAAGA43210.2378885232688669No Hit
GTCCTAAAGTGTGTATTTCTCATTT41130.22643728215803044No Hit
CTTTAGGACGTGAAATATGGCGAGG36160.19907542238838757No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25680.14137878448378852No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23040.12684451692003457No Hit
CTGAAGGACCTGGAATATGGCGAGA21280.11715500521086523No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19410.10685989901987286No Hit
GTCCTACAGTGTGCATTTCTCATTT19400.10680484497607076No Hit
ATTTAGAAATGTCCACTGTAGGACG18170.10003319758841267No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATA307.675117E-419.0177291
GGTATCA13550.014.7369861
CGGTTTC1351.8189894E-1213.369575513
CCAACGA1302.0008883E-1012.41584119
TAGGACC6850.012.3453934
TCCAACG2750.012.08386618
CTACGAG600.005868065611.0854223
TGTCGAG1303.2945536E-810.95515318
ATTTAGA6650.010.8672741
GTATCAA30150.010.5969441
GGCGAGG10400.010.49868919
AGGACCT12950.010.4934815
TGTAGGA23750.010.4816882
AGGACGT24700.010.3877175
ATTTCTA15200.010.36924315
CCAACGT1659.385985E-1010.35759919
CACCTTT6150.010.34441914
TTTAGAA7650.010.3088492
GTCCTAC20900.010.2823141
CTGTAGG23250.010.2654841
Produced by FastQC (version 0.11.5)