FastQCFastQC Report
Thu 26 May 2016
SRR1512340_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512340_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1824121
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT72310.3964101065663955No Hit
GTCCTACAGTGGACATTTCTAAATT65500.35907705683997937No Hit
CTGTAGGACGTGGAATATGGCAAGA59920.3284869808526957No Hit
GTCCTAAAGTGTGTATTTCTCATTT59910.32843215992798724No Hit
CTTTAGGACGTGAAATATGGCGAGG52030.2852332712577729No Hit
TATCAACGCAGAGTACTTTTTTTTT40830.22383383558437187No Hit
GGTATCAACGCAGAGTACTTTTTTT38440.21073163457906574No Hit
GTCCTACAGTGTGCATTTCTCATTT33970.18622668123441372No Hit
CTGAAGGACCTGGAATATGGCGAGA27160.14889363150799756No Hit
CTGTAGGACCTGGAATATGGCGAGA25980.1424247623924071No Hit
GTCCTTCAGTGTGCATTTCTCATTT23550.1291032776882674No Hit
ATTTAGAAATGTCCACTGTAGGACG22530.12351154336801122No Hit
TTTCTAAATTTTCCACCTTTTTCAG21370.11715231610183753No Hit
GAATATGGCAAGAAAACTGAAAATC21300.11676856962887878No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19930.10925810294382884No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18740.10273441290352997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11600.013.432671
TAGGACC10350.012.4856434
GGCGAGG15100.011.96004119
GGACGTA658.012659E-411.6943776
GTACCGT855.3141855E-511.1784486
GTATCAA27800.011.0049371
AGGACCT21500.010.8277955
CCACCTT9000.010.76708813
AATGTCC7600.010.6049658
AGGACGT37850.010.5939755
GACGTGA20400.010.573247
GAACAGT3700.010.528896
ACCTTTT9050.010.50662115
TGTAGGA35050.010.4917352
TCCAACG1451.4375473E-810.4854818
GGACGTG37250.010.4837346
GGACATG8350.010.4689186
TAGAAAT7650.010.4335054
GGACCTG20950.010.38608556
CTAGGAC3300.010.3660413
Produced by FastQC (version 0.11.5)