FastQCFastQC Report
Thu 26 May 2016
SRR1512340_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512340_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1824121
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84910.4654844716989718No Hit
CTGTAGGACGTGGAATATGGCAAGA65060.35666493615281003No Hit
GTCCTACAGTGGACATTTCTAAATT64350.3527726504985141No Hit
GTCCTAAAGTGTGTATTTCTCATTT61070.3347913871941609No Hit
CTTTAGGACGTGAAATATGGCGAGG56150.30781949223763116No Hit
TATCAACGCAGAGTACTTTTTTTTT50610.27744869994918103No Hit
GGTATCAACGCAGAGTACTTTTTTT48770.2673616498028365No Hit
GTCCTACAGTGTGCATTTCTCATTT34620.18979004134045932No Hit
CTGAAGGACCTGGAATATGGCGAGA30090.16495616244755693No Hit
CTGTAGGACCTGGAATATGGCGAGA28350.15541732154829643No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25880.14187655314532316No Hit
ATTTAGAAATGTCCACTGTAGGACG24560.13464019108381514No Hit
TTTCTAAATTTTCCACCTTTTTCAG24000.13157021930014512No Hit
GTCCTTCAGTGTGCATTTCTCATTT22250.1219765574761762No Hit
GAATATGGCAAGAAAACTGAAAATC22130.12131870637967547No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20530.11254735842633247No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCGA250.00603659518.99775110
ACGGTTG250.006039464418.99618713
CGCCACC551.1342014E-515.53722314
ACCGTCC456.741091E-414.7841398
TAGAACG1058.913048E-1114.48162654
GGTATCA11200.014.09881
TAGGACC10900.013.5142484
TCCAACG1850.013.34208618
TATTGCG751.4895088E-512.65787616
ATAGGAC3000.012.3542993
GCGCCAC701.09314555E-412.21183513
CCGGTTT1251.4133548E-912.15856112
CGTGCGC550.00307151712.08947810
TAGAAAT8850.011.8123434
CCAACGA1459.822543E-1111.78556519
GCGTGCG658.0048817E-411.6957359
AATGTCC8300.011.4507098
GGACGTA752.0653893E-411.405226
CGGTTTC1501.7644197E-1011.39771313
GTATCAA27600.011.3735721
Produced by FastQC (version 0.11.5)