FastQCFastQC Report
Thu 26 May 2016
SRR1512339_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512339_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838203
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT77710.4227498268689584No Hit
GTCCTACAGTGGACATTTCTAAATT47700.2594925587652724No Hit
GTCCTAAAGTGTGTATTTCTCATTT45380.2468715370391627No Hit
TATCAACGCAGAGTACTTTTTTTTT45120.24545711219054697No Hit
GGTATCAACGCAGAGTACTTTTTTT44820.24382508351906726No Hit
CTGTAGGACGTGGAATATGGCAAGA43860.23860259177033222No Hit
CTTTAGGACGTGAAATATGGCGAGG42060.228810419741454No Hit
GTCCTACAGTGTGCATTTCTCATTT27230.14813380241464083No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21860.11892048919515419No Hit
CTGTAGGACCTGGAATATGGCGAGA20730.11277318119924731No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19760.10749628849479628No Hit
CTGAAGGACCTGGAATATGGCGAGA19730.1073330856276483No Hit
ATTTAGAAATGTCCACTGTAGGACG18530.10080497094172951No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13700.014.5626731
TCCAACG907.440747E-611.61415218
CCAACGT752.0628629E-411.40672719
CGTGCCC600.005916405511.07321810
GGCGAGG12400.010.962110519
TAGGACC8950.010.9337324
AATGTCC6400.010.8168918
GTATCAA30850.010.7476271
GTCCTAG3200.010.6879621
AAATGTC6450.010.45867357
CTAGTAG1002.3971454E-510.4507373
TGTAGGA29000.010.4507372
AGGACGT29050.010.4000445
GGACGTG28800.010.2264166
ATTTAGA7250.010.2211311
TTAGGAC17050.010.1971993
GGGCTAT1409.682299E-810.1790111
GTCCTAA14000.010.179011
AATCCCG1502.4223482E-810.13931319
GACGTGA15100.010.1304077
Produced by FastQC (version 0.11.5)