##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512339_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1838203 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.02636270313997 31.0 30.0 34.0 16.0 34.0 2 29.925955403184524 31.0 30.0 34.0 23.0 34.0 3 30.583013954389152 33.0 31.0 34.0 26.0 34.0 4 34.24205814047741 37.0 35.0 37.0 31.0 37.0 5 34.42067769446574 37.0 35.0 37.0 32.0 37.0 6 34.57376633592699 37.0 35.0 37.0 32.0 37.0 7 34.52105779394332 37.0 35.0 37.0 32.0 37.0 8 34.52009489702715 37.0 35.0 37.0 32.0 37.0 9 36.10446452323274 39.0 37.0 39.0 32.0 39.0 10 36.04116683521896 39.0 37.0 39.0 32.0 39.0 11 36.05329770433407 39.0 37.0 39.0 32.0 39.0 12 36.00525241227438 39.0 37.0 39.0 32.0 39.0 13 35.972443739891624 39.0 37.0 39.0 32.0 39.0 14 37.216935235118214 40.0 38.0 41.0 32.0 41.0 15 37.14154911073478 40.0 38.0 41.0 31.0 41.0 16 37.071987696679855 40.0 38.0 41.0 31.0 41.0 17 37.0395016219645 40.0 38.0 41.0 31.0 41.0 18 36.82693423958072 40.0 38.0 41.0 30.0 41.0 19 36.94506265086065 40.0 38.0 41.0 31.0 41.0 20 36.88829634159013 40.0 38.0 41.0 31.0 41.0 21 36.832114842593555 40.0 38.0 41.0 31.0 41.0 22 36.75835258673824 40.0 38.0 41.0 31.0 41.0 23 36.637857733884665 40.0 38.0 41.0 30.0 41.0 24 36.51138203995968 40.0 37.0 41.0 30.0 41.0 25 36.39688108440689 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8967618860431941 1101 2 0.12090079703486012 1101 3 -0.2672220740941036 1101 4 -0.4353079363975425 1101 5 -0.7807913993999875 1101 6 -0.7142653082015471 1101 7 -0.8615236595614064 1101 8 -0.8157115137027944 1101 9 -1.0862875888339545 1101 10 -1.0881069603220936 1101 11 -1.131793258608191 1101 12 -1.1595726070501087 1101 13 -1.268812703380334 1101 14 -1.4725158385694996 1101 15 -1.4606079195921353 1101 16 -1.507373621993807 1101 17 -1.6706557676670357 1101 18 -1.3768618087882487 1101 19 -1.319860531119204 1101 20 -1.4831700289986287 1101 21 -1.6824044979532928 1101 22 -1.6876089761556798 1101 23 -1.8959931881599417 1101 24 -1.9744973502924381 1101 25 -1.9785828635112068 1102 1 0.9139688405604964 1102 2 0.16237621174289174 1102 3 -0.2552695739241564 1102 4 -0.2673143338374757 1102 5 -0.3527381743940907 1102 6 -0.4939283631567477 1102 7 -0.5010439062222005 1102 8 -0.6795127913461556 1102 9 -0.7806639699557678 1102 10 -0.7125654854197805 1102 11 -0.6855748978052816 1102 12 -0.9194387144531788 1102 13 -0.9219223859302659 1102 14 -1.058673757092322 1102 15 -1.271573954200754 1102 16 -1.2187813764643778 1102 17 -1.2049926464419016 1102 18 -1.2217081769737632 1102 19 -1.288361816038588 1102 20 -1.3769967112558064 1102 21 -1.3252570292427777 1102 22 -1.2011869685250716 1102 23 -1.359253853434943 1102 24 -1.5035213615296286 1102 25 -1.7205823914233207 1103 1 1.2671596456071583 1103 2 0.27588352717011233 1103 3 -0.06261403790906428 1103 4 -0.1947662564030992 1103 5 -0.21902436601158826 1103 6 -0.3138108432249922 1103 7 -0.5146757155605641 1103 8 -0.5016073506431837 1103 9 -0.5684899930182397 1103 10 -0.5654419135305915 1103 11 -0.5391126293218989 1103 12 -0.6202607996665677 1103 13 -0.7445248468684085 1103 14 -0.8894136015571092 1103 15 -0.953137027946724 1103 16 -0.9816370727778718 1103 17 -0.9524240531328658 1103 18 -0.8778052686890021 1103 19 -1.0811099087486866 1103 20 -1.2366398974620267 1103 21 -1.0287056362677447 1103 22 -1.2643285528752557 1103 23 -1.3947093197102376 1103 24 -1.3082170668925386 1103 25 -1.3391508371314984 1104 1 1.3661036737261725 1104 2 0.6085762283084044 1104 3 0.04692712700951773 1104 4 0.0367548391956376 1104 5 -0.05116394476951314 1104 6 -0.07260633399511818 1104 7 -0.19555970953264534 1104 8 -0.18389632977783066 1104 9 -0.27219564138449215 1104 10 -0.3234703355113666 1104 11 -0.29351723970104615 1104 12 -0.48283025680999714 1104 13 -0.4388138356593103 1104 14 -0.7433416153800394 1104 15 -0.6696432498945413 1104 16 -0.6995277603455037 1104 17 -0.6736847738395539 1104 18 -0.6537481567663619 1104 19 -0.6420402768873927 1104 20 -0.7488058740081911 1104 21 -0.9454578513222032 1104 22 -0.8102117592590048 1104 23 -0.7894428583597062 1104 24 -0.9396685771007114 1104 25 -0.9960317942112553 1105 1 -0.7328277899326743 1105 2 -1.1176910078999889 1105 3 -1.1119564679115612 1105 4 -0.7548629668479307 1105 5 -0.5543626282031511 1105 6 -0.31592167253320724 1105 7 -0.32185253520850665 1105 8 -0.22186323484912407 1105 9 -0.36520689957635 1105 10 -0.2585235627775262 1105 11 -0.4042286621115565 1105 12 -0.3154146873797856 1105 13 -0.44899797418049303 1105 14 -0.6434974998424181 1105 15 -0.5428867618494024 1105 16 -0.5082611638737973 1105 17 -0.669060475348985 1105 18 -0.5059349410976282 1105 19 -0.49289124406361395 1105 20 -0.45202615589143136 1105 21 -0.5807709642809229 1105 22 -0.6122249930047019 1105 23 -0.7049348158717947 1105 24 -0.764435307975809 1105 25 -0.7836832327971237 1106 1 1.2008096021705583 1106 2 0.44210711950449166 1106 3 -0.10145984053423973 1106 4 -0.2002489071377056 1106 5 -0.3248297958717643 1106 6 -0.34097936251591676 1106 7 -0.3401293525086686 1106 8 -0.37618503369024126 1106 9 -0.7165305714802344 1106 10 -0.6779065208850525 1106 11 -0.6032198247829967 1106 12 -0.4871448144875643 1106 13 -0.7182511845820372 1106 14 -0.7533917334139915 1106 15 -0.7988372550542238 1106 16 -0.7518939477870674 1106 17 -0.9296349279877134 1106 18 -0.8342296363058708 1106 19 -0.8045774736355824 1106 20 -0.9348824043108337 1106 21 -0.8531403974385157 1106 22 -0.9954240230069971 1106 23 -1.1371316375640461 1106 24 -1.0224060456775206 1106 25 -1.2701165229688272 1107 1 0.4254862702597215 1107 2 -0.11774915224709304 1107 3 -0.23585591512983228 1107 4 -0.2989257791600366 1107 5 -0.15048925701442784 1107 6 -0.09995920231508393 1107 7 -0.07407347825509447 1107 8 -0.030719508179544164 1107 9 -0.10294271765705076 1107 10 -0.15564192893505435 1107 11 -0.07500134576047657 1107 12 -0.052836183960558 1107 13 -0.006028343999062713 1107 14 -0.022787099050013637 1107 15 -0.1395110378667752 1107 16 -0.32800582126336053 1107 17 -0.3354107683622871 1107 18 -0.09057156287001789 1107 19 -0.1810511396500516 1107 20 -0.23845889074841864 1107 21 -0.1875629764789224 1107 22 -0.2746857712223232 1107 23 -0.18560387261315725 1107 24 -0.2043058444565986 1107 25 -0.1337614540452563 1108 1 -0.023844833251512654 1108 2 -0.3215261669173195 1108 3 -0.6707073258778351 1108 4 -0.2814088841795055 1108 5 -0.4503432801039793 1108 6 -0.3753307548348488 1108 7 -0.41873007818643515 1108 8 -0.5303660147451623 1108 9 -0.5700056535278293 1108 10 -0.5433481794009509 1108 11 -0.6818039968108067 1108 12 -0.7749388665328141 1108 13 -0.7277569753627375 1108 14 -0.9486477427456776 1108 15 -1.0095752082068188 1108 16 -0.8186339832746654 1108 17 -0.930444344388782 1108 18 -0.6943156182469821 1108 19 -0.7656955185696077 1108 20 -0.8503297852522778 1108 21 -0.740507936130534 1108 22 -0.8619388146921594 1108 23 -0.8912813950102674 1108 24 -0.9062913243168325 1108 25 -0.9656232630157291 1109 1 0.989620530807759 1109 2 0.4326131002313467 1109 3 -0.08959577440778688 1109 4 -0.03691411910672571 1109 5 -0.020221189519155303 1109 6 -0.12397921629339947 1109 7 -0.11773619233516541 1109 8 -0.23288131935597534 1109 9 -0.20922085694159165 1109 10 -0.27703833173521275 1109 11 -0.2780296931657915 1109 12 -0.2367706485628318 1109 13 -0.36499056354846715 1109 14 -0.4849709422654769 1109 15 -0.4339640819182904 1109 16 -0.5019045333941392 1109 17 -0.6466424135644715 1109 18 -0.6030555033517757 1109 19 -0.6563199774732169 1109 20 -0.6924200622551524 1109 21 -0.6474143461748199 1109 22 -0.649451945297919 1109 23 -0.8956005466480832 1109 24 -0.9477955819037263 1109 25 -1.153241823247214 1110 1 0.5111080941276533 1110 2 0.21175486775037555 1110 3 -0.14711929430601955 1110 4 -0.03774746461979106 1110 5 -0.1253517453029076 1110 6 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1.0420774977260194 1201 14 1.1459900393220437 1201 15 1.248426918377099 1201 16 1.3095499704322506 1201 17 1.3474373837215268 1201 18 1.3825268664138477 1201 19 1.3621624908734802 1201 20 1.3540678535559039 1201 21 1.458610724973191 1201 22 1.2999490352931176 1201 23 1.3445790108877986 1201 24 1.3166383598800593 1201 25 1.4240642442932767 1202 1 2.413883803860699 1202 2 1.5911232749454207 1202 3 1.25909397025233 1202 4 1.0318540295746033 1202 5 0.8403385299854733 1202 6 0.778430625094785 1202 7 0.8333219928167424 1202 8 0.803392279298123 1202 9 0.8460954361721704 1202 10 0.7904439841267106 1202 11 0.9976140964993334 1202 12 0.8715216131343553 1202 13 0.8541908210509774 1202 14 0.9746743499716004 1202 15 1.1247653514849603 1202 16 1.181260934478935 1202 17 1.1082774502191413 1202 18 1.2547232555260877 1202 19 1.1459581025559231 1202 20 1.139754561343132 1202 21 1.1478562169861704 1202 22 1.1343534899583574 1202 23 1.2058027318677134 1202 24 1.205787141725537 1202 25 1.2905620430124287 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-1.1417572559544382 2314 13 -0.836458138710249 2314 14 -0.7817291549392991 2314 15 -0.8925176404119526 2314 16 -1.080881123194267 2314 17 -1.165515941969538 2314 18 -2.0626284323729394 2314 19 -1.6838235793485765 2314 20 -2.2850285563130512 2314 21 -1.6181854261006308 2314 22 -1.3131517080915387 2314 23 -1.1015581678979487 2314 24 -1.0562692424438893 2314 25 -0.948348347218591 2315 1 -2.546841847156383 2315 2 -1.1959964173965325 2315 3 -0.6076247276861437 2315 4 -0.47988005358156016 2315 5 -0.27467647004925055 2315 6 -0.2703884734129147 2315 7 -0.3766218736077036 2315 8 -0.3445466024799657 2315 9 -0.37229277731348276 2315 10 -0.39430386521581795 2315 11 -0.5702916595443028 2315 12 -0.4668637209508262 2315 13 -0.43588057250519086 2315 14 -0.6036431329777443 2315 15 -1.2248599581084747 2315 16 -1.039265781116491 2315 17 -0.8776151712960854 2315 18 -0.6184559919948001 2315 19 -0.7502546649331236 2315 20 -0.6970135305215308 2315 21 -0.6108268698405936 2315 22 -0.5944678144058528 2315 23 -0.5374385174805738 2315 24 -0.4547751117336887 2315 25 -0.32390517633916005 2316 1 1.0814051419915316 2316 2 0.30428148503287744 2316 3 -0.35009463371133975 2316 4 -0.3813559693104267 2316 5 -0.6496576874524465 2316 6 -0.7391857981020067 2316 7 -0.7447076778919524 2316 8 -0.6765514928645118 2316 9 -0.8559710097929383 2316 10 -0.916596062055973 2316 11 -1.0423883210059088 2316 12 -0.8170188910196927 2316 13 -0.8798117027411294 2316 14 -1.1299056762249862 2316 15 -1.1462276075310314 2316 16 -1.0061740820424205 2316 17 -1.11799124506787 2316 18 -1.00046484618003 2316 19 -0.9997186790777093 2316 20 -1.0723829822006152 2316 21 -0.964191191125181 2316 22 -1.0687561303578619 2316 23 -1.0418314995896552 2316 24 -1.1102962497008662 2316 25 -1.1590464149214128 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 50024.0 3 1.0 4 395.0 5 2013.0 6 2409.0 7 2314.0 8 2104.0 9 2039.0 10 2003.0 11 1959.0 12 1923.0 13 1941.0 14 1994.0 15 1939.0 16 2122.0 17 2296.0 18 2571.0 19 3103.0 20 3790.0 21 4711.0 22 5828.0 23 6768.0 24 8065.0 25 9416.0 26 10569.0 27 11674.0 28 13862.0 29 16565.0 30 21298.0 31 27315.0 32 36255.0 33 49142.0 34 68919.0 35 98965.0 36 172079.0 37 293951.0 38 895881.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.81946496615033 20.21504625093678 12.770247441283662 24.195241341629227 2 13.959763618350129 20.584483744397065 42.94330288362917 22.51244975362363 3 18.08202389189496 26.0111929140647 30.23107212313952 25.675711070900824 4 11.85453386760251 17.57086487005561 39.793287152223066 30.781314110118814 5 12.524160881602814 38.46990630242359 36.107854502802745 12.898078313170853 6 29.841785905732472 38.21421423823237 19.102935410498855 12.841064445536299 7 26.825560162350865 32.184558107034746 23.368316469551022 17.62156526106336 8 24.19930184981968 37.74109072732799 21.322774680405576 16.736832742446754 9 26.449423568410964 15.37838106114898 21.201545440960075 36.97064992947998 10 15.252425014040272 28.85082415035905 34.92287392108004 20.973876914520638 11 32.89570910122071 22.589971997502406 26.287119669096093 18.227199232180784 12 23.82422405194927 24.74919415483461 32.35672152030577 19.06986027291035 13 28.714827785756086 22.8372520989199 26.196014820204393 22.25190529511962 14 20.485339839870683 23.15052602933068 28.401002812434044 27.96313131836459 15 22.045955980685736 32.396750243175 23.960788509258116 21.596505266881145 16 20.90359409211585 28.420182230579634 29.381636142889832 21.294587534414685 17 20.828810916072857 29.26200158956025 28.74340073032373 21.165786764043165 18 20.7013007241858 27.21891847586038 31.72840064243782 20.351380157515994 19 23.13319544995754 26.68588028113393 28.57388753226705 21.60703673664148 20 23.33047371238027 27.408431267175438 29.308589436552936 19.95250558389136 21 22.312383506098556 26.779645313538907 28.105523037179946 22.802448143182584 22 22.886601308079747 28.80613887655508 28.144144831287132 20.16311498407804 23 21.296991087126287 28.454502026446328 29.186934765442658 21.061572120984735 24 22.793425269511875 26.85445840245469 29.806492958492253 20.54562336954119 25 21.92428712005129 27.86399771957052 29.389690230740584 20.82202492963761 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 909.0 1 909.0 2 1342.5 3 1776.0 4 1776.0 5 1776.0 6 3028.5 7 4281.0 8 4281.0 9 4281.0 10 5248.0 11 6215.0 12 6215.0 13 6215.0 14 8689.0 15 11163.0 16 11163.0 17 11163.0 18 18898.0 19 26633.0 20 26633.0 21 26633.0 22 41494.0 23 56355.0 24 56355.0 25 56355.0 26 81303.0 27 106251.0 28 106251.0 29 106251.0 30 134600.5 31 162950.0 32 162950.0 33 162950.0 34 178940.5 35 194931.0 36 194931.0 37 194931.0 38 203554.0 39 212177.0 40 212177.0 41 212177.0 42 219238.0 43 226299.0 44 226299.0 45 226299.0 46 228880.0 47 231461.0 48 231461.0 49 231461.0 50 219483.0 51 207505.0 52 207505.0 53 207505.0 54 180952.5 55 154400.0 56 154400.0 57 154400.0 58 130516.5 59 106633.0 60 106633.0 61 106633.0 62 86622.5 63 66612.0 64 66612.0 65 66612.0 66 51173.5 67 35735.0 68 35735.0 69 35735.0 70 26061.5 71 16388.0 72 16388.0 73 16388.0 74 11273.0 75 6158.0 76 6158.0 77 6158.0 78 4208.5 79 2259.0 80 2259.0 81 2259.0 82 1467.5 83 676.0 84 676.0 85 676.0 86 460.5 87 245.0 88 245.0 89 245.0 90 174.0 91 103.0 92 103.0 93 103.0 94 76.5 95 50.0 96 50.0 97 50.0 98 44.0 99 38.0 100 38.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33358666045045077 2 0.28478900317320777 3 0.3054613663452839 4 0.2947987790249499 5 0.28168814869739633 6 0.2405610261761079 7 0.34642528599942446 8 0.30714779597247965 9 0.25465087370654926 10 0.22810320731714617 11 0.2414314414675637 12 0.24012581853037995 13 0.2589485492081125 14 0.24806835806491448 15 0.33668751492626225 16 0.39310130600374393 17 0.40844237551565304 18 0.11271878024353132 19 0.12795104784400851 20 0.18828170773304145 21 0.18186239495855464 22 0.14622976896458117 23 0.24213865389187159 24 0.3416380018964173 25 0.37912026038473445 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1838203.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.60079923773044 #Duplication Level Percentage of deduplicated Percentage of total 1 83.10084553940712 57.00784421334516 2 11.38551935497556 15.62111454975945 3 2.928952566342354 6.027854609414616 4 1.0607394947026278 2.910703084785064 5 0.4803532060124409 1.6476306924429818 6 0.2597606688476722 1.0691873696086647 7 0.1587394145010978 0.7622755493713281 8 0.11379137852057988 0.6244943610299915 9 0.07988511193639103 0.4932164273428787 >10 0.3764577281038976 4.630353014387765 >50 0.028536571525120107 1.3622374998172908 >100 0.02155100100493158 2.9878389262151495 >500 0.0029526995483586337 1.4606589088925106 >1k 0.0018354618814121226 2.9691651955584186 >5k 7.980269049617924E-5 0.4254255980286981 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7771 0.4227498268689584 No Hit GTCCTACAGTGGACATTTCTAAATT 4770 0.2594925587652724 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4538 0.2468715370391627 No Hit TATCAACGCAGAGTACTTTTTTTTT 4512 0.24545711219054697 No Hit GGTATCAACGCAGAGTACTTTTTTT 4482 0.24382508351906726 No Hit CTGTAGGACGTGGAATATGGCAAGA 4386 0.23860259177033222 No Hit CTTTAGGACGTGAAATATGGCGAGG 4206 0.228810419741454 No Hit GTCCTACAGTGTGCATTTCTCATTT 2723 0.14813380241464083 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2186 0.11892048919515419 No Hit CTGTAGGACCTGGAATATGGCGAGA 2073 0.11277318119924731 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1976 0.10749628849479628 No Hit CTGAAGGACCTGGAATATGGCGAGA 1973 0.1073330856276483 No Hit ATTTAGAAATGTCCACTGTAGGACG 1853 0.10080497094172951 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.440095571599002E-5 2 0.0 0.0 0.0 0.0 5.440095571599002E-5 3 0.0 0.0 0.0 5.440095571599002E-5 5.440095571599002E-5 4 0.0 0.0 0.0 5.440095571599002E-5 5.440095571599002E-5 5 0.0 0.0 0.0 5.440095571599002E-5 5.440095571599002E-5 6 1.0880191143198004E-4 0.0 0.0 5.440095571599002E-5 5.440095571599002E-5 7 1.0880191143198004E-4 0.0 0.0 5.440095571599002E-5 5.440095571599002E-5 8 1.0880191143198004E-4 0.0 0.0 5.440095571599002E-5 5.440095571599002E-5 9 1.0880191143198004E-4 0.0 0.0 1.0880191143198004E-4 1.0880191143198004E-4 10 1.0880191143198004E-4 0.0 0.0 1.0880191143198004E-4 1.0880191143198004E-4 11 1.0880191143198004E-4 0.0 0.0 1.6320286714797006E-4 2.720047785799501E-4 12 1.0880191143198004E-4 0.0 0.0 1.6320286714797006E-4 2.720047785799501E-4 13 1.0880191143198004E-4 0.0 0.0 1.6320286714797006E-4 2.720047785799501E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1370 0.0 14.562673 1 TCCAACG 90 7.440747E-6 11.614152 18 CCAACGT 75 2.0628629E-4 11.406727 19 CGTGCCC 60 0.0059164055 11.073218 10 GGCGAGG 1240 0.0 10.9621105 19 TAGGACC 895 0.0 10.933732 4 AATGTCC 640 0.0 10.816891 8 GTATCAA 3085 0.0 10.747627 1 GTCCTAG 320 0.0 10.687962 1 AAATGTC 645 0.0 10.4586735 7 CTAGTAG 100 2.3971454E-5 10.450737 3 TGTAGGA 2900 0.0 10.450737 2 AGGACGT 2905 0.0 10.400044 5 GGACGTG 2880 0.0 10.226416 6 ATTTAGA 725 0.0 10.221131 1 TTAGGAC 1705 0.0 10.197199 3 GGGCTAT 140 9.682299E-8 10.179011 1 GTCCTAA 1400 0.0 10.17901 1 AATCCCG 150 2.4223482E-8 10.139313 19 GACGTGA 1510 0.0 10.130407 7 >>END_MODULE