FastQCFastQC Report
Thu 26 May 2016
SRR1512339_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512339_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838203
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT92680.5041880575757954No Hit
GGTATCAACGCAGAGTACTTTTTTT57360.31204388198691874No Hit
TATCAACGCAGAGTACTTTTTTTTT56290.3062229797253078No Hit
GTCCTACAGTGGACATTTCTAAATT47410.2579149310495087No Hit
GTCCTAAAGTGTGTATTTCTCATTT47300.2573165205366328No Hit
CTGTAGGACGTGGAATATGGCAAGA46590.2534540526807975No Hit
CTTTAGGACGTGAAATATGGCGAGG45150.24562031505769494No Hit
GTCCTACAGTGTGCATTTCTCATTT26740.1454681555845573No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26230.1426937068430418No Hit
CTGTAGGACCTGGAATATGGCGAGA22750.12376217425387728No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22360.12164053698095367No Hit
CTGAAGGACCTGGAATATGGCGAGA21920.11924689492945012No Hit
TTTCTAAATTTTCCACCTTTTTCAG20760.11293638406639527No Hit
ATTTAGAAATGTCCACTGTAGGACG19740.10738748658336429No Hit
GAATATGGCAAGAAAACTGAAAATC18570.10102257476459345No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18540.1008593718974455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13850.014.4913571
TAGGACC9200.011.9817614
GGCGAGG11100.011.71844419
TCCAACG1302.6393536E-911.68554518
CCAACGA907.5107346E-611.60439519
GCGCCAC752.0769688E-411.39836713
TGTCGAG1105.0067683E-711.22077918
CCGTTTC600.005882616611.08174613
CCGTTTA600.005882616611.08174613
GGACCTG16300.010.9034426
GTATCAA32200.010.9005111
AGGACCT17000.010.788745
AGTCGTC1605.4023985E-1010.692088
AGAAGCG803.7602618E-410.6909155
TATGTCG1252.2582572E-710.63384516
TTAGTAC909.4934E-510.5580663
ATCCCGT909.5248906E-510.55461810
GTGCGCC909.5248906E-510.55461811
GCCGGTT1002.401378E-510.44907211
CGGTTTC1002.4028208E-510.44850313
Produced by FastQC (version 0.11.5)