FastQCFastQC Report
Thu 26 May 2016
SRR1512338_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512338_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2203031
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69840.31701778141115583No Hit
GTCCTACAGTGGACATTTCTAAATT46470.21093665953860838No Hit
GTCCTAAAGTGTGTATTTCTCATTT45600.20698755487326323No Hit
CTGTAGGACGTGGAATATGGCAAGA42160.1913727042424732No Hit
TATCAACGCAGAGTACTTTTTTTTT42090.1910549601889397No Hit
GGTATCAACGCAGAGTACTTTTTTT41940.19037408007422502No Hit
CTTTAGGACGTGAAATATGGCGAGG40500.18383763097296407No Hit
GTCCTACAGTGTGCATTTCTCATTT28110.12759693349753137No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG500.001489169913.31266516
GGTATCA14400.012.9988081
TATGTCG1405.0931703E-1112.22591616
TAGGACC9350.012.1943744
GCGAACC951.0491349E-611.99170311
GTGTAGG3600.011.8770831
GTCGAGG1459.640644E-1111.79894319
AGGACGT26750.011.0110255
GGACGTG26600.010.9657086
GTCCTAT2950.010.9510741
CGAACCG1053.4898967E-610.85136912
TAGAAAT7750.010.7887424
TGTAGGA28300.010.6770282
AATACGG1252.2140921E-710.64940314
GACGTGG11850.010.5836517
GGCGAGG13150.010.55275119
GTATCAA30700.010.4920671
GTAGGAC26300.010.4412333
GGCGAAC1106.109456E-610.35481810
GTCCTAA15600.010.2934721
Produced by FastQC (version 0.11.5)