##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512338_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2203031 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.95504420954585 31.0 30.0 34.0 16.0 34.0 2 29.84004219641031 31.0 30.0 34.0 22.0 34.0 3 30.49232580022705 33.0 31.0 34.0 26.0 34.0 4 34.14932881107892 37.0 35.0 37.0 30.0 37.0 5 34.31535144081041 37.0 35.0 37.0 31.0 37.0 6 34.46112015672952 37.0 35.0 37.0 32.0 37.0 7 34.40074833263808 37.0 35.0 37.0 32.0 37.0 8 34.4017937105742 37.0 35.0 37.0 32.0 37.0 9 35.97332901806647 39.0 37.0 39.0 32.0 39.0 10 35.899883387932356 39.0 37.0 39.0 31.0 39.0 11 35.924571646971835 39.0 37.0 39.0 31.0 39.0 12 35.86531964370906 39.0 37.0 39.0 31.0 39.0 13 35.83726602122258 39.0 37.0 39.0 31.0 39.0 14 37.062449870201554 40.0 38.0 41.0 31.0 41.0 15 36.99011316681427 40.0 38.0 41.0 31.0 41.0 16 36.91702295610003 40.0 38.0 41.0 31.0 41.0 17 36.87494865029135 40.0 38.0 41.0 31.0 41.0 18 36.65795805869277 40.0 37.0 41.0 30.0 41.0 19 36.76960560246315 40.0 38.0 41.0 30.0 41.0 20 36.70091160768959 40.0 38.0 41.0 30.0 41.0 21 36.644405820889496 40.0 38.0 41.0 30.0 41.0 22 36.56957074140128 40.0 38.0 41.0 30.0 41.0 23 36.445506213938884 40.0 37.0 41.0 30.0 41.0 24 36.311900740389035 40.0 37.0 41.0 30.0 41.0 25 36.19730135436133 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2730249720078533 1101 2 0.3553736518763344 1101 3 -0.1413064884834725 1101 4 -0.15118898812579573 1101 5 -0.3817774801756997 1101 6 -0.4399728924950139 1101 7 -0.4261484225492964 1101 8 -0.6094003074787651 1101 9 -0.4487650969633421 1101 10 -0.5449545544568153 1101 11 -0.6189218285884834 1101 12 -0.8735925375717031 1101 13 -0.7410624085714232 1101 14 -1.070381902431265 1101 15 -1.1507784766191733 1101 16 -1.0739847854897278 1101 17 -1.3269478384545437 1101 18 -0.941203900050283 1101 19 -1.1830718515362548 1101 20 -1.4403617631792542 1101 21 -1.3517304989205314 1101 22 -1.334623964214778 1101 23 -1.290164680387278 1101 24 -1.556059046082602 1101 25 -1.6549039408226207 1102 1 1.1876041432251014 1102 2 0.2885857843892303 1102 3 -0.1874817812744638 1102 4 -0.16342760723556893 1102 5 -0.3172786655463611 1102 6 -0.4132079332948493 1102 7 -0.2843292559492241 1102 8 -0.5165862286493947 1102 9 -0.562549581482493 1102 10 -0.592870610679789 1102 11 -0.4911646209133238 1102 12 -0.6166250442532899 1102 13 -0.7856474400481233 1102 14 -0.8781582891373532 1102 15 -0.8587066973200947 1102 16 -0.9571081261927858 1102 17 -1.1568125197363415 1102 18 -1.0327043040530128 1102 19 -0.9955848373384484 1102 20 -1.2458277869932175 1102 21 -1.1983111263234605 1102 22 -1.2336627928732256 1102 23 -1.3664892322106184 1102 24 -1.392967581639283 1102 25 -1.5188074063669603 1103 1 1.0548353050358514 1103 2 0.1808708775204373 1103 3 -0.3662030423859939 1103 4 -0.3843059532857822 1103 5 -0.5323244547063766 1103 6 -0.5744844058087608 1103 7 -0.5466881321632044 1103 8 -0.6222244649063526 1103 9 -1.0307007234834487 1103 10 -0.8511076230944852 1103 11 -0.8188017995288206 1103 12 -0.9083930682264949 1103 13 -0.9380837926960055 1103 14 -1.1875629041474696 1103 15 -1.1674456697290836 1103 16 -1.4184181746260478 1103 17 -1.3310014514345312 1103 18 -1.0876340884116757 1103 19 -1.3144247053850862 1103 20 -1.3009174766677774 1103 21 -1.5050149783208084 1103 22 -1.514630702688308 1103 23 -1.6125637875395356 1103 24 -1.5541454248901019 1103 25 -1.6821026310318317 1104 1 1.334941780963998 1104 2 0.5491680031146444 1104 3 0.09493842695219712 1104 4 0.09065221159640657 1104 5 -0.06063535256945585 1104 6 -0.15410811188188944 1104 7 -0.046162416523536365 1104 8 -0.1630525781055141 1104 9 -0.19267667384404774 1104 10 -0.3824247258829985 1104 11 -0.3311968415120248 1104 12 -0.28002219435382614 1104 13 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0.01650135280019782 1107 11 -0.04554923555788548 1107 12 -0.15409221375965387 1107 13 -0.05201650771020638 1107 14 -0.1627065511655843 1107 15 -0.2822320910455005 1107 16 -0.23638859722017713 1107 17 -0.2866543561274426 1107 18 -0.10382693857386016 1107 19 -0.21041989116602622 1107 20 -0.2858521272566321 1107 21 -0.3523479542002832 1107 22 -0.18236592824549547 1107 23 -0.15221169548376423 1107 24 -0.3479534873760812 1107 25 -0.28592111291766287 1108 1 -0.2686814503192636 1108 2 -0.5376996079195457 1108 3 -0.812954181813776 1108 4 -0.6736704985884785 1108 5 -0.5785629510594106 1108 6 -0.5689280548411446 1108 7 -0.5759115713658858 1108 8 -0.6529615732653369 1108 9 -0.7731506721793693 1108 10 -0.6520680831608203 1108 11 -0.6978315558941048 1108 12 -0.8665193955703785 1108 13 -0.7927691674543951 1108 14 -1.0770213080736255 1108 15 -1.0323528849119086 1108 16 -0.897457873642125 1108 17 -0.963501590631644 1108 18 -0.8910364635120942 1108 19 -1.0187068062274207 1108 20 -1.106144129335192 1108 21 -1.0026880003298615 1108 22 -1.1949772637242475 1108 23 -1.089558879847111 1108 24 -1.1533905788421066 1108 25 -1.1153401172115451 1109 1 0.8258557828488584 1109 2 0.1970767323101157 1109 3 -0.23412386770940685 1109 4 -0.21323767210265743 1109 5 -0.33984495340627063 1109 6 -0.39466787143880566 1109 7 -0.44567720714238135 1109 8 -0.45275107439813667 1109 9 -0.5880038803975225 1109 10 -0.6862114151273602 1109 11 -0.7474017716270964 1109 12 -0.695077327163915 1109 13 -0.6248243279834043 1109 14 -0.7900646795323212 1109 15 -0.8260318722498567 1109 16 -0.8593349037563129 1109 17 -1.0060979448819651 1109 18 -0.8225927757382223 1109 19 -0.9335620929801678 1109 20 -1.0001424551700566 1109 21 -1.0882947151303526 1109 22 -1.0368245896894948 1109 23 -0.9476985158012212 1109 24 -1.2203726788800893 1109 25 -1.3070788869836036 1110 1 0.39238166932575425 1110 2 -0.14946779680267497 1110 3 -0.37999655645736397 1110 4 -0.3160793294881685 1110 5 -0.2262947815608385 1110 6 -0.21807918742799615 1110 7 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10 -0.5611309793433819 2314 11 -1.4874779849686206 2314 12 -1.1206573023178095 2314 13 -0.9153709423522685 2314 14 -0.949874878999104 2314 15 -1.0016214438638755 2314 16 -1.2076265593725068 2314 17 -1.088091321579661 2314 18 -2.2488333363110797 2314 19 -1.820700044711721 2314 20 -2.295251750126859 2314 21 -1.6056267945262306 2314 22 -1.3397687836566305 2314 23 -1.095175868155323 2314 24 -1.0084053696577158 2314 25 -1.033553950145759 2315 1 -2.756850481987872 2315 2 -1.1677054814096728 2315 3 -0.6051915275637256 2315 4 -0.5066868904495792 2315 5 -0.3862348073944091 2315 6 -0.43285262447321315 2315 7 -0.3868334255728101 2315 8 -0.4182864169936167 2315 9 -0.4820077250492929 2315 10 -0.5559377768029847 2315 11 -0.598752587244924 2315 12 -0.5762154779240873 2315 13 -0.6326161162188342 2315 14 -0.6511490096662769 2315 15 -1.263260495195908 2315 16 -0.9818429022009028 2315 17 -0.9084388975087307 2315 18 -0.6683097453743585 2315 19 -0.7510266078339782 2315 20 -0.6058152706366116 2315 21 -0.639552521328298 2315 22 -0.6356348676240202 2315 23 -0.5555329286680148 2315 24 -0.4930508243937197 2315 25 -0.3645795428012377 2316 1 1.0905912248901295 2316 2 0.3471368155896535 2316 3 -0.15614839757725107 2316 4 -0.2930491226846925 2316 5 -0.444109518458653 2316 6 -0.6220800342240622 2316 7 -0.4941562560247732 2316 8 -0.6036452299336759 2316 9 -0.7953455322266194 2316 10 -0.8467906832037784 2316 11 -0.9680914170151595 2316 12 -0.7494859644013587 2316 13 -0.7305428090894424 2316 14 -0.7244663649853607 2316 15 -0.8458427553392838 2316 16 -0.7105311072307856 2316 17 -0.6887245844035661 2316 18 -0.7327889153697811 2316 19 -0.8268290769697799 2316 20 -0.7615367845002012 2316 21 -0.6142135211259117 2316 22 -0.8567254203567956 2316 23 -0.8960246254266053 2316 24 -0.8437993411013949 2316 25 -0.8306454361320164 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 64037.0 3 5.0 4 538.0 5 2637.0 6 3136.0 7 2838.0 8 2662.0 9 2646.0 10 2468.0 11 2560.0 12 2498.0 13 2511.0 14 2606.0 15 2525.0 16 2659.0 17 2877.0 18 3264.0 19 3894.0 20 4616.0 21 5769.0 22 7146.0 23 8588.0 24 10023.0 25 11766.0 26 13269.0 27 14762.0 28 17232.0 29 20582.0 30 25963.0 31 33863.0 32 44128.0 33 59976.0 34 84686.0 35 120446.0 36 207227.0 37 354265.0 38 1052363.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.34914132582154 19.678650281988407 12.512623542171628 24.459584850018427 2 14.39444366033664 20.158303777527877 42.115269917729385 23.3319826444061 3 18.349692958189035 25.326293116206845 29.77374308672081 26.55027083888331 4 12.28129449525786 17.101478443520307 38.59685150587776 32.020375555344074 5 13.156895542283973 37.94553713943873 35.648161216040386 13.249406102236916 6 31.12033195020747 37.124505901873 18.601844511654026 13.153317636265506 7 27.60884856414471 31.756373834432466 22.76590064635811 17.86887695506472 8 24.880256618332144 37.07012831827377 20.902836408545568 17.146778654848525 9 26.83206344857342 15.183136937739766 20.681728087035157 37.30307152665165 10 15.672787153484483 28.652487303777903 34.20155881077544 21.473166731962174 11 33.722802925049365 22.26638991951063 25.418431415201283 18.592375740238722 12 23.84726667397817 24.45437918752227 31.850312020609334 19.848042117890227 13 29.126780102900963 22.178279740599272 25.849087691573846 22.84585246492592 14 20.945946560959737 22.448492902556357 27.820977849786328 28.784582686697586 15 22.742824397889876 31.456675356360186 23.499980185130102 22.300520060619835 16 21.86768615536355 27.794011141997803 28.421255573619636 21.91704712901901 17 21.543360282268633 28.742441268111897 28.045111925074817 21.669086524544653 18 21.46120485344369 26.9771593293453 30.52953144790283 21.03210436930818 19 23.620645066283302 26.551000214982707 27.797627190903277 22.030727527830717 20 23.884897387045704 27.03767091357207 28.384376397133032 20.693055302249196 21 23.05909501673882 26.399582314018538 27.393178311073275 23.148144358169368 22 23.550870564105175 27.96587748734225 27.447112014611985 21.03613993394059 23 22.19738533061988 27.762977781499266 28.246538712178854 21.793098175701996 24 23.373751711684463 26.515051434808395 28.739398489253396 21.371798364253742 25 22.70426078297783 27.229910017476982 28.44204227743054 21.623786922114647 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 909.0 1 909.0 2 1450.5 3 1992.0 4 1992.0 5 1992.0 6 3219.5 7 4447.0 8 4447.0 9 4447.0 10 5240.0 11 6033.0 12 6033.0 13 6033.0 14 8580.5 15 11128.0 16 11128.0 17 11128.0 18 18969.0 19 26810.0 20 26810.0 21 26810.0 22 42734.5 23 58659.0 24 58659.0 25 58659.0 26 85254.5 27 111850.0 28 111850.0 29 111850.0 30 142897.5 31 173945.0 32 173945.0 33 173945.0 34 194073.0 35 214201.0 36 214201.0 37 214201.0 38 228659.5 39 243118.0 40 243118.0 41 243118.0 42 254600.0 43 266082.0 44 266082.0 45 266082.0 46 272899.5 47 279717.0 48 279717.0 49 279717.0 50 270461.0 51 261205.0 52 261205.0 53 261205.0 54 232987.0 55 204769.0 56 204769.0 57 204769.0 58 177001.5 59 149234.0 60 149234.0 61 149234.0 62 122949.0 63 96664.0 64 96664.0 65 96664.0 66 74718.0 67 52772.0 68 52772.0 69 52772.0 70 38538.0 71 24304.0 72 24304.0 73 24304.0 74 17024.0 75 9744.0 76 9744.0 77 9744.0 78 6695.0 79 3646.0 80 3646.0 81 3646.0 82 2411.5 83 1177.0 84 1177.0 85 1177.0 86 782.5 87 388.0 88 388.0 89 388.0 90 262.0 91 136.0 92 136.0 93 136.0 94 100.5 95 65.0 96 65.0 97 65.0 98 50.5 99 36.0 100 36.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.350789435101004 2 0.3005404826350605 3 0.3211938461147392 4 0.31184309253932424 5 0.29618284990088656 6 0.253877498773281 7 0.3614565568982007 8 0.32160237418356796 9 0.26554324473872587 10 0.24193940076195022 11 0.2541044588115192 12 0.25328740267386163 13 0.2708541096335004 14 0.26150335605808545 15 0.34997237896334643 16 0.4080741487523326 17 0.42559546370432366 18 0.11865470799094519 19 0.1295487898263801 20 0.19663817712960008 21 0.19291603250249315 22 0.14493668041893193 23 0.25342357869680454 24 0.35519245984282566 25 0.39563673865687776 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2203031.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.7250812111985 #Duplication Level Percentage of deduplicated Percentage of total 1 81.5404264643531 55.223320042940735 2 12.035644661216192 16.302300242199887 3 3.3294353775339722 6.764588439927769 4 1.2752900356764254 3.454764849360726 5 0.6055216177548376 2.0504500368791336 6 0.32333559556974056 1.313875768105915 7 0.19172323797691815 0.9089130303442503 8 0.13237752140422882 0.7172222710110852 9 0.09834413234633156 0.5994327914820143 >10 0.4204808894825971 4.914752524679243 >50 0.024045675306623524 1.1501196364840416 >100 0.019335896980560496 2.6739468548231824 >500 0.0026252813590732713 1.343510045883084 >1k 0.0013462981328577984 2.2644093647439063 >5k 6.731490664288993E-5 0.31839410113491934 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6984 0.31701778141115583 No Hit GTCCTACAGTGGACATTTCTAAATT 4647 0.21093665953860838 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4560 0.20698755487326323 No Hit CTGTAGGACGTGGAATATGGCAAGA 4216 0.1913727042424732 No Hit TATCAACGCAGAGTACTTTTTTTTT 4209 0.1910549601889397 No Hit GGTATCAACGCAGAGTACTTTTTTT 4194 0.19037408007422502 No Hit CTTTAGGACGTGAAATATGGCGAGG 4050 0.18383763097296407 No Hit GTCCTACAGTGTGCATTTCTCATTT 2811 0.12759693349753137 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.539200764764545E-5 2 0.0 0.0 0.0 0.0 4.539200764764545E-5 3 0.0 0.0 0.0 0.0 4.539200764764545E-5 4 0.0 0.0 0.0 0.0 4.539200764764545E-5 5 0.0 0.0 0.0 0.0 4.539200764764545E-5 6 0.0 0.0 0.0 0.0 4.539200764764545E-5 7 0.0 0.0 0.0 0.0 4.539200764764545E-5 8 0.0 0.0 0.0 0.0 4.539200764764545E-5 9 0.0 0.0 0.0 0.0 4.539200764764545E-5 10 0.0 0.0 0.0 0.0 4.539200764764545E-5 11 0.0 0.0 0.0 0.0 4.539200764764545E-5 12 0.0 0.0 0.0 0.0 1.3617602294293635E-4 13 0.0 0.0 0.0 0.0 2.2696003823822724E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGGCG 50 0.0014891699 13.312665 16 GGTATCA 1440 0.0 12.998808 1 TATGTCG 140 5.0931703E-11 12.225916 16 TAGGACC 935 0.0 12.194374 4 GCGAACC 95 1.0491349E-6 11.991703 11 GTGTAGG 360 0.0 11.877083 1 GTCGAGG 145 9.640644E-11 11.798943 19 AGGACGT 2675 0.0 11.011025 5 GGACGTG 2660 0.0 10.965708 6 GTCCTAT 295 0.0 10.951074 1 CGAACCG 105 3.4898967E-6 10.851369 12 TAGAAAT 775 0.0 10.788742 4 TGTAGGA 2830 0.0 10.677028 2 AATACGG 125 2.2140921E-7 10.649403 14 GACGTGG 1185 0.0 10.583651 7 GGCGAGG 1315 0.0 10.552751 19 GTATCAA 3070 0.0 10.492067 1 GTAGGAC 2630 0.0 10.441233 3 GGCGAAC 110 6.109456E-6 10.354818 10 GTCCTAA 1560 0.0 10.293472 1 >>END_MODULE