FastQCFastQC Report
Thu 26 May 2016
SRR1512338_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512338_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2203031
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82640.37511955120014195No Hit
TATCAACGCAGAGTACTTTTTTTTT54280.2463878175114195No Hit
GGTATCAACGCAGAGTACTTTTTTT50620.22977434271238126No Hit
GTCCTACAGTGGACATTTCTAAATT47470.21547586030337293No Hit
GTCCTAAAGTGTGTATTTCTCATTT46920.21297929988275244No Hit
CTGTAGGACGTGGAATATGGCAAGA44380.2014497299402505No Hit
CTTTAGGACGTGAAATATGGCGAGG42030.1907826081430538No Hit
GTCCTACAGTGTGCATTTCTCATTT27300.12392018087807206No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23780.10794219418610088No Hit
CTGTAGGACCTGGAATATGGCGAGA22800.10349377743663161No Hit
CTGAAGGACCTGGAATATGGCGAGA22470.10199584118425933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13300.014.8011981
GGACCGT1058.913048E-1114.482136
TAGGACC8200.012.7465244
TCCAACG1105.007714E-711.22091518
TGTCGAG1701.2732926E-1111.1701418
GTATCAA31750.010.8727921
CCGTGCA803.7601186E-410.69115459
GGCGAGG13600.010.54205919
ACCGTGC1002.3887802E-510.4542888
TATGTCG1659.385985E-1010.35753116
TGTAGGA26000.010.3418062
CACCTTT7350.010.33498414
AGGACGT27950.010.1988775
TTAGGAC18700.010.1625183
AATGTCC6750.010.137498
GTAGGAC25500.010.1354173
GTCGAGG1502.4665496E-810.12782519
CCACCTT7700.010.11620613
ACCTTTT7550.010.06075215
AAATGTC6550.010.0117397
Produced by FastQC (version 0.11.5)