FastQCFastQC Report
Thu 26 May 2016
SRR1512337_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512337_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1934228
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62270.3219372276691269No Hit
TATCAACGCAGAGTACTTTTTTTTT36610.18927448056795787No Hit
GGTATCAACGCAGAGTACTTTTTTT36380.18808537566408923No Hit
GTCCTACAGTGGACATTTCTAAATT29610.15308433131978236No Hit
GTCCTAAAGTGTGTATTTCTCATTT28310.14636330360226407No Hit
CTGTAGGACGTGGAATATGGCAAGA26150.13519605754854133No Hit
CTTTAGGACGTGAAATATGGCGAGG23910.12361520978912517No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACT307.709383E-419.00522218
CCGTGCG250.006073812518.9776129
CCGAAAC400.00524380314.26466517
GGTATCA9950.013.6516991
TAGGACC5400.013.3698764
TACACCG701.09153734E-412.2138365
GTGCGCT658.0607296E-411.68610911
GTCCTAT1800.011.6097781
GGCGAGG8700.011.58060219
CGTGCGC1157.136259E-811.55818710
TTAGGAC11800.011.2707713
CCCCGTA855.277046E-511.18622715
GGATAGA1201.2777127E-711.0820611
AAGGCGT1553.1286618E-1011.0315656
GACGTGA10150.010.9503357
GTCCTAG2000.010.9237451
AGGACGG700.001492141710.8567435
TGGCGAG16150.010.82650418
ATAGGAC1855.456968E-1210.7833853
TTATACC1158.822972E-710.7387334
Produced by FastQC (version 0.11.5)